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Zeitschrift für Kristallographie - Crystalline Materials

Editor-in-Chief: Pöttgen, Rainer

Ed. by Antipov, Evgeny / Boldyreva, Elena V. / Friese, Karen / Huppertz, Hubert / Jahn, Sandro / Tiekink, E. R. T.

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Volume 225, Issue 2-3


The strong phase object approximation may allow extending crystallographic phases of dynamical electron diffraction patterns of 3D protein nano-crystals

Jan Pieter Abrahams
Published Online: 2010-04-16 | DOI: https://doi.org/10.1524/zkri.2010.1216


Simulations suggest that the strong phase object approximation of dynamical scattering may allow extracting crystallographic phase information from single 2D electron diffraction patterns of 3D protein crystals using probabilitistic procedures. Unlike other phasing methods, the procedure does not requires any additional knowledge – like real space images, atomicity, non-crystallographic symmetry, the presence or location of disordered solvent or the availability of a support with a known structure. In specific cases, the availability of precession electron diffraction data can further improve the phases.

Keywords: Phase information; Diffraction patterns; Precession electron diffraction

About the article

* Correspondence address: Leiden Institute of Chemistry, Gorlaeus Laboratories, Einsteinweg 55, 2333 CC Leiden, Niederlande,

Published Online: 2010-04-16

Published in Print: 2010-03-01

Citation Information: Zeitschrift für Kristallographie International journal for structural, physical, and chemical aspects of crystalline materials, Volume 225, Issue 2-3, Pages 67–76, ISSN (Print) 0044-2968, DOI: https://doi.org/10.1524/zkri.2010.1216.

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