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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

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1865-7125
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Volume 29, Issue 7-8

Issues

Reactions of a Monospecific Antiserum to Ferredoxin-NADP+ - Reductase with Chloroplast Preparations

Georg H. Schmid
  • Max-Planck-Institut für Züchtungsforschungs (Erwin-Baur-Institut), Abteilung Menke, Köln-Vogelsang
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alfons Radunz
  • Max-Planck-Institut für Züchtungsforschungs (Erwin-Baur-Institut), Abteilung Menke, Köln-Vogelsang
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-1974-7-814

Abstract

A monospecific antiserum to ferredoxin-NADP+-reductase inhibits the diaphorase activity of soluble ferredoxin-NADP+-reductase from chloroplasts. Two states of the molecular structure of the lamellar system have been observed, one of which is the state described earlier by Berzborn. Stroma-freed chloroplasts in this condition are not agglutinated by the antiserum, although a specific adsorption of antibodies to reductase onto the lamellar system was demonstrated by the Coombs test. However, a second type of chloroplast preparations gives direct agglutination upon addition of the antiserum. Apparently, agglutination in this state is not sterically hindered by neighboring protein structures. This type of chloroplast preparations appears swollen under the light micro­ scope, but exhibits high rates of electron transport.

Chloroplasts of three types of tobacco have been used which differ in the morphology of their lamellar systems. The green type contains a normal ratio of grana and intergrana regions whereas the other two types have extended intergrana regions with either only small grana or no partitions at all. Comparison of the maximal degree of inhibition of the NADP+-reduction in chloroplasts from these types of tobacco by the antiserum, leads to the conclusion that ferredoxin-NADP+-reductase is located in the grana and the intergrana regions of the lamellar system, in the outer surface of the thylakoid membrane.

: Antibodies; Ferredoxin-NADP+-Reductase; Chlorophyll-deficient Tobacco Mutants

About the article

Received: 1974-04-23

Published Online: 2014-06-02

Published in Print: 1974-08-01


Citation Information: Zeitschrift für Naturforschung C, Volume 29, Issue 7-8, Pages 384–391, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-1974-7-814.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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