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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

12 Issues per year


IMPACT FACTOR 2017: 0.882
5-year IMPACT FACTOR: 0.912

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.288
Source Normalized Impact per Paper (SNIP) 2017: 0.448

Online
ISSN
1865-7125
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Volume 32, Issue 7-8

Issues

Preparation of Human Erythrocyte Ghosts in Isotonic Solution: Haemoglobin Content and Polypeptide Composition

M. Saleemuddin / U. Zimmermann / F. Schneeweiß
Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-1977-7-823

Abstract

Human red blood cell ghosts were prepared by dielectric breakdown of the cell membrane and subsequent electrical haemolysis in isotonic and isoionic solutions. Almost complete removal of haemoglobin (to about 0.1%) could be achieved by subjecting the erythrocytes suspended in isotonic solution to three consecutive electric field pulses of 16 kV/cm in the presence of 10 mᴍ EDTA, followed by dilution with EDTA-free isoionic medium. The cup-shaped ghost cells were electrically homogeneous. The polypeptide composition of the electrically prepared ghost cells, as analyzed by polyacrylamide electrophoresis in 1% SDS, revealed a pattern similar to ghost cells prepared by osmotic haemolysis. However, the band 6, corresponding to glyceraldehyde-3-phosphate-dehydrogenase protomer, was absent from the electrically prepared ghost cells. Also the band 8, which is eluted at low ionic strength, appeared to be very prominent in the electrical preparations.

: Electrical Haemolysis; Ghost Cell Preparation; Protein Composition

About the article

Received: 1977-05-04

Published Online: 2014-06-02

Published in Print: 1977-08-01


Citation Information: Zeitschrift für Naturforschung C, Volume 32, Issue 7-8, Pages 627–631, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-1977-7-823.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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