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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

TMV Inoculation of Tobacco Protoplasts in the Presence of Protoplast Fusion Agents

  • Alan C. Cassells and Frances M. Cocker

Abstract

Two methods used to induce protoplast fusion, involving polyethylene glycol (PEG) and high calcium/high pH, respectively, have been investigated in procedures for the inoculation of isolated tobacco protoplasts with TMV. It has been shown that inoculation in the presence of fusion agents involves two stages. Firstly, virus precipitation on to the protoplast surface and secondly, entrapment of virus particles between protoplasts in zones (putatively) of transient protoplast fusion.

Received: 1980-6-24
Revised: 1980-8-22
Published Online: 2014-6-2
Published in Print: 1980-12-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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