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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

The Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin

  • Sofia K. Mastronicolis , Vassilios M. Kapoulas and Hans Kröger

Abstract

By coupling 5-Methyl-cytidine to bovine serum albumin, a conjugate containing 18 mol of 5-Methyl-cytidine per mol of BSA was prepared. Antibodies to this conjugate were produced by immunization of rabbits. Specificity of the antibody was assesed by gel diffusion in agar containing excessive amounts of the carrier BSA. A slight cross-reactivity with cytidine was eliminated by adsorption on the cross-reacting antigen.

The isolation of 7 S immunoglobulin from the total globulin fraction was accomplished by chromatography on DEAE Sephadex-A-50, and a method for the rapid quantitation of the antibodies showed that 12.7% of the IgG protein are monospecific against 5-Methyl-cytidine bovine serum albumine.

Received: 1980-8-18
Revised: 1981-2-6
Published Online: 2014-6-2
Published in Print: 1981-6-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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