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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Neutral Red Fluorescence of Chromatin: Specificity and Binding Mechanism

  • Roberto H. Espelosin and Juan C. Stockert

Abstract

Neutral Red, Chromatin Fluorescence, Nucleic Acid Cytochemistry, Intercalative Binding to DNA After treatment with neutral red at low concentrations (10-6, 10-5 m), the chromatin o f chicken erythrocytes shows an intensive red fluorescence, which is reduced or prac­ tically abolished when nuclei become stained by using the dye at concentrations higher than 10~* M. Both the fluores­ cence and staining reactions are dependent on the DNA content of chromatin. Neutral red fluorescence o f nuclei increases considerably after treatment with inorganic cations (Al3+, Ba2+), while previous treatments with meth­ ylene blue reduce the fluorescence intensity. The possi­ bility that chromatin fluorescence depends on the inter­ calative binding of neutral red is suggested.

Received: 1981-8-5
Revised: 1981-10-21
Published Online: 2014-6-2
Published in Print: 1982-2-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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