Abstract
Neutral Red, Chromatin Fluorescence, Nucleic Acid Cytochemistry, Intercalative Binding to DNA After treatment with neutral red at low concentrations (10-6, 10-5 m), the chromatin o f chicken erythrocytes shows an intensive red fluorescence, which is reduced or prac tically abolished when nuclei become stained by using the dye at concentrations higher than 10~* M. Both the fluores cence and staining reactions are dependent on the DNA content of chromatin. Neutral red fluorescence o f nuclei increases considerably after treatment with inorganic cations (Al3+, Ba2+), while previous treatments with meth ylene blue reduce the fluorescence intensity. The possi bility that chromatin fluorescence depends on the inter calative binding of neutral red is suggested.
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