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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus


IMPACT FACTOR 2018: 1.000

CiteScore 2018: 0.99

SCImago Journal Rank (SJR) 2018: 0.246
Source Normalized Impact per Paper (SNIP) 2018: 0.437

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1865-7125
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Volume 37, Issue 10

Issues

Mechanism of Bleaching in Leaves Treated with Chlorosis-Inducing Herbicides

Jürgen Feierabend
  • Botanisches Institut, J. W. Goethe-Universität, Postfach 11 19 32, D-6000 Frankfurt/M., Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Theresia Winkelhüsener
  • Botanisches Institut, J. W. Goethe-Universität, Postfach 11 19 32, D-6000 Frankfurt/M., Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Petra Kemmerich
  • Botanisches Institut, J. W. Goethe-Universität, Postfach 11 19 32, D-6000 Frankfurt/M., Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ulrike Schulz
  • Botanisches Institut, J. W. Goethe-Universität, Postfach 11 19 32, D-6000 Frankfurt/M., Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-1982-1009

Abstract

Bleaching of chlorophyll was studied in the leaves of rye seedlings (Secale cereale L.) treated with four chlorosis-inducing herbicides of different potency (weak photodestructions, group 1: aminotriazole, haloxidine; strong photodestructions, group 2: San 6706, difunone). Chlorophyll deficiency and particularly the inactivation of a chloroplast marker enzyme, NADP-dependent glyceraldehyde-3-P dehydrogenase, that occurred in the presence of group 2 herbicides were stronger in red, than in blue, light.

When grown in white light of low intensity (10 lx) herbicide-treated leaves contained chloro­ phyll, 70 S ribosomes and unimpaired activities of NADP-dependent glyceraldehyde-3-P de hydrogenase. At 10 lx only the leaves treated with SAN 6706 and difunone were strongly carotenoid-deficient but not those treated with group 1 herbicides. After all herbicide treatments 10 lx-grown leaf tissue was, however, not capable of photosynthetic O2-evolution indicating some disorder of photosynthetic electron transport. Leaf segments grown at 10 lx were exposed to a high light intensity of 30000 lx at either 0 ° C or 30 °C. In treatments with group 1 herbicides chlorophyll accumulation was stopped in bright light at 30 °C but breakdown was not apparent. Only at 0 °C and in the presence of high, growth-reducing, herbicide concentrations chlorophyll was slightly degraded. The RNAs o f the 70S ribosomes were, however, clearly destroyed at 30000 lx and 30 °C in aminotriazole-treated leaves. In leaves treated with group 2 herbicides chlorophyll was rapidly degraded at 30000 lx both at 0 ° C and 30 °C, however, only in the presence of O2, indicating a true photooxidative and mainly photochemical nature o f the reactions involved. This chlorophyll breakdown was accompanied by the photodestruction of 70S ribosomes and the inactivation of NADP-glyceraldehyde-3-P dehydrogenase.In treatments with group 1 herbicides photoinactivation of the latter enzyme did not occur, although it was clearly localized in the bleached plastids, as demonstrated by gradient separation of organelles.

In the presence of group 2 herbicides the chlorosis was originating from a direct photo­ oxidation of chlorophyll, accompanied by a massive destruction of other plastid constituents and functions. In treatments with group 1 herbicides photodestructions appeared to be much weaker and insufficient to affect chlorophyll directly. Mediated through some photodestructive inter­ ference with obviously more sensitive plastid components, such as their ribosomes, further chlorophyll accumulation was, however, prevented.

Keywords: Bleaching Herbicides; Carotenoids; Chlorophyll; Photooxidation; Plastid rRNA

About the article

Received: 1982-07-07

Published Online: 2014-06-02

Published in Print: 1982-10-01


Citation Information: Zeitschrift für Naturforschung C, Volume 37, Issue 10, Pages 898–907, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-1982-1009.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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