Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Yeast Aminopeptidase II: Rapid Purification Using Affinity Chromatography of the Periplasmic Enzyme

  • Joseph Knüver and Klaus-Heinrich Röhm

Periplasmic aminopeptidase II of yeast was isolated from protoplast supernatants by ammonium sulfate precipita­tion, DEAE-Sephacel chromatography and affinity chromatography on immobilized bestatin. This isolation method is more rapid than conventional procedures and, in addition, avoids contact of the enzyme with yeast protein-ases. Thus the preparation obtained is likely to represent the native periplasmic from of aminopeptidase II.

Received: 1982-3-25
Published Online: 2014-6-2
Published in Print: 1982-9-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Downloaded on 19.3.2024 from https://www.degruyter.com/document/doi/10.1515/znc-1982-0918/html
Scroll to top button