Periplasmic aminopeptidase II of yeast was isolated from protoplast supernatants by ammonium sulfate precipitation, DEAE-Sephacel chromatography and affinity chromatography on immobilized bestatin. This isolation method is more rapid than conventional procedures and, in addition, avoids contact of the enzyme with yeast protein-ases. Thus the preparation obtained is likely to represent the native periplasmic from of aminopeptidase II.
Received: 1982-3-25
Published Online: 2014-6-2
Published in Print: 1982-9-1
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