Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus


IMPACT FACTOR 2017: 0.882
5-year IMPACT FACTOR: 0.912

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.288
Source Normalized Impact per Paper (SNIP) 2017: 0.448

Online
ISSN
1865-7125
See all formats and pricing
More options …
Volume 40, Issue 1-2

Issues

Production of Four Interfacial Active Rhamnolipids from n-Alkanes or Glycerol by Resting Cells of Pseudomonas species DSM 2874

C. Syldatk
  • Institut für Biochemie und Biotechnologie, Technische Universität, Bültenweg 17, D-3300 Braunschweig. Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ S. Lang
  • Institut für Biochemie und Biotechnologie, Technische Universität, Bültenweg 17, D-3300 Braunschweig. Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ U. Matulovic
  • Institut für Biochemie und Biotechnologie, Technische Universität, Bültenweg 17, D-3300 Braunschweig. Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ F. Wagner
  • Institut für Biochemie und Biotechnologie, Technische Universität, Bültenweg 17, D-3300 Braunschweig. Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-1985-1-213

In a simple phosphate buffer or a sodium chloride solution resting cells of Pseudomonas spec. DSM 2874 produced up to 15 g/l of different rhamnolipids. The rhamnolipid composition of the organic crude extract depended on the temperature during the cultivation and on the C-source. The optimal sodium chloride concentration for rhamnolipid formation was about 100 mᴍ/l and the optimal phosphate buffer concentration about 65 mᴍ/l. The optimal pH-value for the production of rhamnolipids from n-alkanes or glycerol was in the range pH 6.0-7.2. While rhamnolipid formation with glycerol as the sole C-source showed a wide optimum ranging from 27° up to 37 °C, production of rhamnolipids from n-alkanes had a sharp optimum at 37 °C. The addition of multivalent cations, different N-sources and EDTA caused an inhibition of rhamnolipid formation, while the n-alkane concentration had no influence. Specific rhamnolipid formation decreased with increasing cell concentration. Various C-sources were suitable for the formation of rhamnolipids by resting cells of Pseudomonas spec. DSM 2874. Yields, which were comparable to those obtained on n-alkanes or glycerol, were found for stearic acid, fatty alcohols and vegetable oils.

A study of the time course of glycolipid production of resting cells was carried out in a 20 1- biorcactor with an intcnsor system and with n-tetradecane as the sole C-source.

Keywords: Pseudomonas; Interfacial-Active; Rhamnolipids; n-Alkanes; Resting Cells

About the article

Received: 1984-08-20

Revised: 1984-10-23

Published Online: 2014-06-02

Published in Print: 1985-02-01


Citation Information: Zeitschrift für Naturforschung C, Volume 40, Issue 1-2, Pages 61–67, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-1985-1-213.

Export Citation

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
U. Matulovic, D. Rasch, and F. Wagne
Biotechnology Letters, 1986, Volume 8, Number 7, Page 485
[2]
H. J. Busscher, T. R. Neu, and H. C. van der Mei
Applied Microbiology and Biotechnology, 1994, Volume 41, Number 1, Page 4
[4]
E. Haba, A. Pinazo, O. Jauregui, M. J. Espuny, M. R. Infante, and A. Manresa
Biotechnology and Bioengineering, 2003, Volume 81, Number 3, Page 316
[5]
[6]
Andreas Wittgens and Frank Rosenau
Applied Microbiology and Biotechnology, 2018, Volume 102, Number 19, Page 8175
[7]
Michael Powalla, Siegmund Lang, and Victor Wray
Applied Microbiology and Biotechnology, 1989, Volume 31-31, Number 5-6, Page 473

Comments (0)

Please log in or register to comment.
Log in