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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

The Presence of a Specialized-β-GIucosidase: Linamarase, in the Leaves of Trifolium repens is Controlled by the Gene Li

  • P. Kakes and H. Eeltink

Abstract

Cyanogenesis, β-Glucosidase, Linamarase, Trifolium repens Linamarase was purified by a three-step procedure from leaves of a clone of Trifolium repens. The partially purified enzyme was used to produce antilinamarin. The antiserum contained anti-linamarase as well as antibodies against one or more other β-glucosidases. By incubation with an extract of a plant without linamarase activity pure antilinamarase could be obtained. Comparison of the titration curves of crude and purified antilinamarase shows that linamarase is an enzyme antigenically different from other β-glucosidases, and that this enzyme is absent from plants recessive for the gene Li.

Received: 1985-1-28
Published Online: 2014-6-2
Published in Print: 1985-7-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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