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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus


IMPACT FACTOR 2018: 1.000

CiteScore 2018: 0.99

SCImago Journal Rank (SJR) 2018: 0.246
Source Normalized Impact per Paper (SNIP) 2018: 0.437

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1865-7125
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Volume 44, Issue 7-8

Issues

Molecular Evolution of H+-ATPases. I. Methanococcus and Sulfolobus are Monophyletic with Respect to Eukaryotes and Eubacteria

Johann Peter Gogarten
  • Biology Department, Thimann Laboratories, University of California, Santa Cruz, California 95064
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/ Thomas Rausch
  • Biology Department, Thimann Laboratories, University of California, Santa Cruz, California 95064
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/ Paul Bernasconi
  • Biology Department, Thimann Laboratories, University of California, Santa Cruz, California 95064
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/ Henrik Kibak
  • Biology Department, Thimann Laboratories, University of California, Santa Cruz, California 95064
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/ Lincoln Taiz
  • Biology Department, Thimann Laboratories, University of California, Santa Cruz, California 95064
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Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-1989-7-816

The classification of methanogenic bacteria as archaebacteria based on 16 s rRNA sequence analysis is currently in dispute. To provide an alternative molecular marker, the polymerase chain reaction technique was used to amplify a 930 bp fragment of Methanococcus thermolithotrophicus genomic DNA corresponding to the catalytic domain of the membrane H+ -ATPase. The deduced amino acid sequence was 54-58% identical to the ~ 70 kDa subunits of Sulfolobus acidocaldarius and the eukaryotic vacuolar-type H+-A T P ase, and only 29% identical to the beta subunit of the eubacterial-type F0F1-ATPases. Interestingly, a highly conserved aspartate residue in the phosphorylation domain of E 1 E 2-A TPases (P-type) is conserved in the Methanococcus sequence, but is absent from all other known vacuolar and F0F1-ATPases. This suggests that the H+-ATPase of M. thermolithotrophicus, like that of M. voltae, may have a phosphorylated intermediate, despite belonging to the vacuolar-type class of proton pumps. Phylogenetic analysis using Felsenstein’s maximum likelihood method and Lake’s evolutionary parsimony method confirmed that the H+- ATPases of the two archaebacteria, Methanococcus and Sulfolobus, when compared to eukaryotic vacuolar-type ATPases and eubacterial F0F1-ATPases, form a monophyletic group.

Keywords: Molecular Evolution; Methanobacteria; Archaebacteria; ATPase; Polymerase Chain Reaction

About the article

Received: 1989-04-06

Published Online: 2014-06-02

Published in Print: 1989-08-01


Citation Information: Zeitschrift für Naturforschung C, Volume 44, Issue 7-8, Pages 641–650, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-1989-7-816.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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