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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

12 Issues per year


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Online
ISSN
1865-7125
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Volume 49, Issue 9-10

Issues

Stereospecific Reduction of /R(+)-Thioctic Acid by Porcine Heart Lipoamide Dehydrogenase/Diaphorase

H. Schempp
  • Lehrstuhl für Phytopathologie, Labor für Angewandte Biochemie, Technische Universität München, D-85350 Weihenstephan, Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ H. Ulrich / E. F. Elstner
  • Lehrstuhl für Phytopathologie, Labor für Angewandte Biochemie, Technische Universität München, D-85350 Weihenstephan, Bundesrepublik Deutschland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-1994-9-1023

Abstract

R(+)-thioctic acid is the naturally occurring cofactor in α-ketoacid dehydrogenases. We show both photo­ metrically by NADH + H+ oxidation and by HPLC prod­uct analysis that this enantiomer is rapidly reduced by NADH + H+ catalyzed by porcine heart lipoamide dehydrogenase/diaphorase. The racemate exhibits approxi­ mately 40% activity as compared to the R (+) form while the S (-) enantiomer photometrically shows little activity and yields no detectable reduced lipoic acid.

Keywords: Thioctic Acid; Lipoic Acid; Diaphorase; Stereospecificity

About the article

Received: 1994-04-25

Published Online: 2014-06-02

Published in Print: 1994-10-01


Citation Information: Zeitschrift für Naturforschung C, Volume 49, Issue 9-10, Pages 691–692, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-1994-9-1023.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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