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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

12 Issues per year


IMPACT FACTOR 2016: 0.835

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.288
Source Normalized Impact per Paper (SNIP) 2017: 0.448

Online
ISSN
1865-7125
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Volume 55, Issue 11-12

Issues

PCR-Based Identification of Bacillus thuringiensis Isolated from Soil Samples in Nigeria

Abiodun A. Ogunjimi
  • Department of Biochemistry, College of Medicine of the University of Lagos, P. M. B. 12003, Lagos, Nigeria
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/ George O. Gbenle
  • Department of Biochemistry, College of Medicine of the University of Lagos, P. M. B. 12003, Lagos, Nigeria
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/ Daniel K. 0lukoya
  • Genetics Division, Nigerian Institute of Medical Research, P. M. B. 2013, Yaba, Lagos, Nigeria
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/ Ezekiel O. Akinrimisi
  • Department of Biochemistry, College of Medicine of the University of Lagos, P. M. B. 12003, Lagos, Nigeria
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Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-2000-11-1223

Six isolates of Bacillus thuringiensis isolated from soil samples confirmed to be toxic to mosquito larvae were differentiated using a PCR-Based technique. Three of these isolates initially identified using a serological technique were further differentiated with the PCR amplification of the δ-endotoxin target sequences. Using the total DNA of isolates as tem plate, at least four isolates yielded amplicons one or all the crystal protein genes, cryl a, b, c, or II with sizes ranging from 238-1070 bp. None of these isolates yielded an amplicon for any of Cry IV A, B and D tested. Of the four isolates identified by PCR technique one isolate remained unidentified by serology.

Keywords: Polymerase Chain Reaction (PCR); Bacillus thuringiensis; Mosquito Larvicidal Activity

About the article

Received: 2000-03-01

Revised: 2000-07-12

Published Online: 2014-06-02

Published in Print: 2000-12-01


Citation Information: Zeitschrift für Naturforschung C, Volume 55, Issue 11-12, Pages 987–990, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2000-11-1223.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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