Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board Member: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

12 Issues per year


IMPACT FACTOR 2016: 0.835

CiteScore 2016: 0.72

SCImago Journal Rank (SJR) 2016: 0.222
Source Normalized Impact per Paper (SNIP) 2016: 0.413

Online
ISSN
1865-7125
See all formats and pricing
More options …
Volume 58, Issue 5-6 (Jun 2003)

Issues

Evaluation of Sage Phenolics for Their Antileishmanial Activity and Modulatory Effects on Interleukin-6, Interferon and Tumour Necrosis Factor-α-Release in RAW 264.7 Cells

Oliver A. Radtke
  • Institut für Pharmazie, Pharmazeutische Biologie, Freie Universität Berlin, Königin-Luise-Straße 2+4, D-14195 Berlin, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lai Yeap Foo / Yinrong Lu / Albrecht F. Kiderlen / Herbert Kolodziej
  • Corresponding author
  • Institut für Pharmazie, Pharmazeutische Biologie, Freie Universität Berlin, Königin-Luise-Straße 2+4, D-14195 Berlin, Germany
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-2003-5-618

Abstract

A series of sage phenolics was tested for activity against a panel of Leishmania parasites and for immunomodulatory effects on macrophage functions including release of tumour necrosis factor (TNF), interleukin-6 (IL-6), and interferon (IFN)-like activities. For this, functional bioassays were employed including an in vitro model for leishmaniasis in which macrophage- like RAW 264.7 cells were infected with Leishmania parasites, an extracellular Leishmania growth-inhibition assay, a fibroblast-lysis assay for TNF-activity, a cell proliferation assay using IL-6 sensitive murine B9 hybridoma cells, and a virus protection assay for IFN-like activity. Whereas none of the test samples exhibited marked activities against extracellular Leishmania promastigotes (IC50 > 700 to > 2800 nм; > 500 μg/ml), caffeic acid, salvianolic acids K and L as well as the methyl ester of salvianolic acid I showed pronounced antileishmanial activities against intracellular amastigote stages within RAW cells (IC50 3-23 nм vs. 10-11 nм for the reference Pentostam®). Noteworthy, the phenolic samples showed no cytotoxicity against the host cells (IC50 > 600 to > 2200 nм; > 400 μg/ml). Tested sage phenolics activated Leishmania-infected RAW 264.7 for release of TNF ranging 22-117 U/ml and IL-6 ranging 3-42 U/ml. In contrast, their TNF- or IL-6-inducing potential in experiments with non-infected host cells was negligible. Furthermore, caffeic acid and salvianolic acid K induced a modest release of IFN-like activity (5-9 and 2-4 U/ml, respectively) as reflected by inhibition of the cytopathic effect of encephalomyocarditis virus on L929 cells. The results support the emerging picture that plant polyphenols may be credited for the profound healthbeneficial properties of various herbal medicines and agricultural products.

Keywords: Salvia officinalis; Leishmania; Immunomodulation

About the article

Received: 2002-12-09

Revised: 2003-01-15

Published Online: 2014-06-02

Published in Print: 2003-06-01


Citation Information: Zeitschrift für Naturforschung C, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2003-5-618.

Export Citation

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Chuan-Chen Ho, Shih-Shen Lin, Ming-Yung Chou, Fang-Lung Chen, Chao-Chin Hu, Chung-Shih Chen, Guan-Yu Lu, and Chi-Chiang Yang
Journal of Antimicrobial Chemotherapy, 2005, Volume 56, Number 2, Page 372
[2]
Evan C. Palmer-Young and Lucy Thursfield
PeerJ, 2017, Volume 5, Page e3297
[3]
Piu Saha, Debanjan Mukhopadhyay, and Mitali Chatterjee
International Immunopharmacology, 2011, Volume 11, Number 11, Page 1668
[4]
Vassiliki G. Kontogianni, Goran Tomic, Ivana Nikolic, Alexandra A. Nerantzaki, Nisar Sayyad, Stanislava Stosic-Grujicic, Ivana Stojanovic, Ioannis P. Gerothanassis, and Andreas G. Tzakos
Food Chemistry, 2013, Volume 136, Number 1, Page 120
[5]
Daniela Vergara, Daniela Ávila, Elizabeth Escobar, Catalina Carrasco-Pozo, Andrés Sánchez, and Martin Gotteland
Nutrition Journal, 2015, Volume 14, Number 1
[6]
Eileen Ryan, S. Aisling Aherne, Michael N. O'Grady, Laura McGovern, Joseph P. Kerry, and Nora M. O'Brien
Journal of Medicinal Food, 2009, Volume 12, Number 4, Page 893

Comments (0)

Please log in or register to comment.
Log in