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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus


IMPACT FACTOR 2018: 1.000

CiteScore 2018: 0.99

SCImago Journal Rank (SJR) 2018: 0.246
Source Normalized Impact per Paper (SNIP) 2018: 0.437

Online
ISSN
1865-7125
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Volume 63, Issue 1-2

Issues

Effect of Cell Culture on 18S rRNA Gene Sequences in the Cultural Course of Taxus chinensis Cells

Fu Xiang
  • College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
  • Other articles by this author:
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/ Long J. Yu
  • Corresponding author
  • College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
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/ Wu Chen / Zhi Liu
  • College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
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Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-2008-1-223

Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T. chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.

Keywords: 18S rRNA Gene; Cultured Cells; Taxus chinensis

About the article

Received: 2007-07-05

Revised: 2007-08-14

Published Online: 2014-06-02

Published in Print: 2008-02-01


Citation Information: Zeitschrift für Naturforschung C, Volume 63, Issue 1-2, Pages 127–132, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2008-1-223.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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