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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus


IMPACT FACTOR 2018: 1.000

CiteScore 2018: 0.99

SCImago Journal Rank (SJR) 2018: 0.246
Source Normalized Impact per Paper (SNIP) 2018: 0.437

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1865-7125
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Volume 64, Issue 5-6

Issues

Effect of DNA Methylation on 18S rRNA Gene Sequences during Culture of Taxus chinensis Cells

Fu Xiang
  • Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
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/ Liqing Li
  • Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
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/ Wenwen Jin
  • Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
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/ Longjiang Yu
  • Corresponding author
  • Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
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Published Online: 2014-06-02 | DOI: https://doi.org/10.1515/znc-2009-5-620

Cell suspension culture has rapidly become an alternative source of taxol, an anticancer compound. To investigate the role of DNA methylation in the cultural course of Taxus chinensis cells, analyses of 18S rRNA gene sequences of cultured T. chinensis cells and related species were conducted. The phylogenetic analysis of 18S rRNA gene sequences indicated that HG-1 (the cultured T. chinensis cells), like T. mairei (the natural variety of T. chinensis), should be a new variety of T. chinensis, and cell culture can change the 18S rRNA gene sequence at the level of species despite 18S rRNA is the most conserved gene. The analyses of the CpG and TpG+CpA relative abundance and GC content of the 18S rRNA gene sequences made clear that DNA methylation contributed to changes of the 18S rRNA gene sequence of HG-1 at the level of species, which can make HG-1 to become a new variety of T. chinensis.

Keywords: 18S rRNA Gene; DNA Methylation; Taxus chinensis Cell

About the article

Received: 2008-12-15

Published Online: 2014-06-02

Published in Print: 2009-06-01


Citation Information: Zeitschrift für Naturforschung C, Volume 64, Issue 5-6, Pages 418–420, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2009-5-620.

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© 1946 – 2014: Verlag der Zeitschrift für Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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