Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

12 Issues per year

IMPACT FACTOR 2017: 0.882
5-year IMPACT FACTOR: 0.912

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.288
Source Normalized Impact per Paper (SNIP) 2017: 0.448

See all formats and pricing
More options …
Volume 70, Issue 9-10


Selective nematocidal effects of essential oils from two cultivated Artemisia absinthium populations

Juan José García-Rodríguez
  • Corresponding author
  • CEI Campus Moncloa (UCM-UPM and CSIC), Madrid, Spain
  • Facultad de Farmacia, Departamento de Parasitología, Universidad Complutense de Madrid, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ María-Fé Andrés / Alexandra Ibañez-Escribano
  • CEI Campus Moncloa (UCM-UPM and CSIC), Madrid, Spain
  • Facultad de Farmacia, Departamento de Parasitología, Universidad Complutense de Madrid, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Luis F. Julio / Jesús Burillo / Francisco Bolás-Fernández
  • CEI Campus Moncloa (UCM-UPM and CSIC), Madrid, Spain
  • Facultad de Farmacia, Departamento de Parasitología, Universidad Complutense de Madrid, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Azucena González-Coloma
Published Online: 2015-10-07 | DOI: https://doi.org/10.1515/znc-2015-0109


Essential oils (EOs) obtained from two crops and populations of thujone-free cultivated Artemisia absinthium were tested against two nematode models, the mammalian parasite Trichinella spiralis, and the plant parasitic root knot nematode Meloidogyne javanica. The EOs were characterized by the presence of (Z)-epoxyocimene and chrysanthenol as major components and showed time and population dependent quantitative and qualitative variations in composition. The EOs showed a strong ex vivo activity against the L1 larvae of the nematode Trichinella spiralis with a reduction of infectivity between 72 and 100% at a dose range of 0.5–1 mg/ml in absence of cytotoxicity against mammalian cells. Moreover, the in vivo activity of the EO against T. spiralis showed a 66% reduction of intestinal adults. However, these oils were not effective against M. javanica.

Keywords: Artemisia absinthium; essential oil; Meloidogyne; nematocidal; Trichinella


  • 1.

    Giarratana F, Muscolino C, Beninati C, Giuffrida A, Panebianco A. Activity of Thymus vulgaris essential oil against Anisakis larvae. Exp Parasitol 2014;142:7–10.Google Scholar

  • 2.

    De Aquino Mesquita ME, Silva Junior JB, Pannasol AM, de Oliveira EF, Vasconcelos OL, de Paula HC, et al. Anthelmintic activity of Eucalyptus staigeriana encapsulated oil on sheep gastrointestinal nematodes. Parasitol Res 2013;112: 3161–5.Web of ScienceCrossrefGoogle Scholar

  • 3.

    Zhu L, Dai J, Yang L, Qiu J. Anthelmintic activity of Arisaema franchetianum and Ariasema lobatum essential oils against Haemonchus contortus. J Ethnopharmacol 2013;148:311–6.CrossrefPubMedGoogle Scholar

  • 4.

    Macedo IT, Bevilaqua CM, de Oliveira AL, Camurça-Vasconcelos AL, Vieira Ld, Oliveira FR, et al. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Vet Parasitol 2010;173:93–8.Web of ScienceGoogle Scholar

  • 5.

    Macedo IT, de Oliveira LM, Camurça-Vasconcelos AL, Ribeiro WL, dos Santos JM, de Morais SM, et al. In vitro effects of Coriandrum sativum, Tagetes minuta, Alpinia zerumbet and Lantana camara essential oil on Haemonchus contortus. Rev Bras Parasitol Vet 2013;22:463–9.CrossrefGoogle Scholar

  • 6.

    Andrés MF, González-Coloma A, Sanz J, Burillo J, Sainz P. Nematicidal activity of essential oils: a review. Phytochem Rev 2012;11:371–90.CrossrefGoogle Scholar

  • 7.

    Bai PH, Bai CQ, Liu QZ, Du SS, Liu SL. Nematocidal activity of the essential oil of Rhododendron anthopogonoides aerial parts and its constituent compound against Meloidogyne incognita. Z Naturforsch C 2013;68:307–12.CrossrefGoogle Scholar

  • 8.

    Li W, Sun YN, Yan XT, Yang SY, Lee SJ, Byun HJ, et al. Isolation of nematocidal triterpenoid saponins from Pulsatilla koreana root and their activities against Meloidogyne incognita. Molecules 2013;18:5306–16.CrossrefGoogle Scholar

  • 9.

    Mattei D, Dias-Arieira CR, Biela F, Roldi M, da Silva TR, Rampim L, et al. Essential oil of Rosmarinus officinalis in the control of Meloidogyne javanica and Pratylenchus brachyurus in soybean. Biosci J 2014;30:469–76.Google Scholar

  • 10.

    Jasmer DP, Goverse A, Geert Smant G. Parasitic nematode interactions with mammals and plants. Annu Rev Phytopathol 2003;41:245–70.CrossrefPubMedGoogle Scholar

  • 11.

    Pozio E. The broad spectrum of Trichinella hosts: from cold-to warm-blooded animals. Vet Parasitol 2005;132:3–11.Google Scholar

  • 12.

    Dupouy-Camet J. Trichinellosis: a worldwide zoonosis. Vet Parasitol 2000;93:191–200.CrossrefGoogle Scholar

  • 13.

    Gottstein B, Pozio E, Nockler K. Epidemiology, diagnosis, treatment, and control of trichinellosis. Clin Microbiol Rev 2009;22:127–45.PubMedCrossrefGoogle Scholar

  • 14.

    García JJ, Bolas F, Torrado JJ. Bioavailability and efficacy characteristics of two different oral liquid formulations of albendazole. Int J Pharm 2003;250:351–8.Google Scholar

  • 15.

    Prichard RK. Markers for benzimidazole resistance in human parasitic nematodes? Parasitology 2007;134:1087–92.PubMedCrossrefGoogle Scholar

  • 16.

    Castagnone-Sereno P, Danchin EG, Perfus-Barbeoch L, Abad P. Diversity and evolution of root-knot nematodes, Genus Meloidogyne: new insights from the genomic era. Annu Rev Phytopathol 2013;51:203–20.Web of ScienceCrossrefGoogle Scholar

  • 17.

    Ramasubramaniaraja R, Niranjan Babu M. Antihelminthic studies and medicinal herbs – an overview. Int J Pharm Sci Rev Res 2010;5:39–47.Google Scholar

  • 18.

    D’Addabbo T, Pinarosa Avato T, Carbonara T, Argentieri MP, Radicci V, Leonetti P, et al. Nematocidal potential of Artemisia annua and its main metabolites. Eur J Plant Pathol 2013;137:295–304.Google Scholar

  • 19.

    Squires JM, Ferreira JF, Lindsay DS, Zajac AM. Effects of artemisinin and Artemisia extracts on Haemonchus contortus in gerbils (Meriones unguiculatus). Vet Parasitol 2011;175: 103–8.Web of ScienceGoogle Scholar

  • 20.

    Lachenmeier DW. Wormwood (Artemisia absinthium L.). A curious plant with both neurotoxic and neuroprotective properties? J Ethnopharmacol 2010;131:224–7.Web of ScienceGoogle Scholar

  • 21.

    Tariq KA, Chishti MZ, Ahmad F, Shawl AS. Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes. Vet Parasitol 2009;160:83–8.Google Scholar

  • 22.

    Baykan Erel S, Reznicek G, Şenol SG, Karabay Yavaşogulu NÜ, Konyalioglu S, Zeybek AU. Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia. Turk J Biol 2012;36:75–84.Web of ScienceGoogle Scholar

  • 23.

    Gandomi Nasrabadi H, Abbaszadeh S, Tayyar Hashtjin N, Yamrali I. Study of chemical composition of essential oil of afsantine (Artemisia absinthium) and inhibitory effects of the essential oil and its aqueous and alcoholic extracts on some food borne bacterial pathogens. J Med Plants 2012;11:120–7.Google Scholar

  • 24.

    Juteau F, Jerkovic I, Masotti V, Milos M, Mastelic J, Bessière JM, et al. Composition and antimicrobial activity of the essential oil of Artemisia absinthium from Croatia and France. Planta Med 2003;69:158–61.CrossrefGoogle Scholar

  • 25.

    Martínez-Díaz R, Ibáñez-Escribano A, Burillo J, de las Heras L, del Prado G, Agulló-Ortuño MT, et al. Trypanocidal and trichomonacidal components of cultivated Artemisia absinthium Linnaeus (Asteraceae) essential oil. Mem Inst Oswaldo Cruz 2015;110:1–7.Web of ScienceGoogle Scholar

  • 26.

    Bailén M, Julio LF, Diaz CE, Sanz J, Martínez-Díaz RA, Cabrera R, et al. Chemical composition and biological effects of essential oils from Artemisia absinthium L. cultivated under different environmental conditions. Ind Crop Prod 2013;49:102–7.CrossrefWeb of ScienceGoogle Scholar

  • 27.

    Olsen RW. Absinthe and gamma-aminobutyric acid receptors. Proc Natl Acad Sci USA 2000;97:4417–8.CrossrefGoogle Scholar

  • 28.

    Burillo J. Cultivo experimental de ajenjo Artemisia absinthiumL. como potencial insecticida de origen natural. In: Burillo J, González-Coloma A, editors. Insecticidas y Repelentes De Origen Natural. Zaragoza: Centro de Investigación y Tecnología Agroalimentaria, 2009:19.Google Scholar

  • 29.

    González-Coloma A, Bailén M, Diaz CE, Fraga BM, Martínez-Díaz R, Zuñiga GE, et al. Major components of Spanish cultivated Artemisia absinthium populations: antifeedant, antiparasitic, and antioxidant effects. Ind Crop Prod 2012;37:401–7.Web of ScienceCrossrefGoogle Scholar

  • 30.

    Julio LF, Burillo J, Giménez C, Cabrera R, Díaz CE, Sanz J, et al. Chemical and biocidal characterization of two cultivated Artemisia absinthium populations with different domestication levels. Ind Crop Prod 2015;76:787–92.CrossrefGoogle Scholar

  • 31.

    Wakelin D, Lloyd M. Immunity to primary and challenge infections of Trichinella spiralis: a re-examination of conventional parameters. Parasitology 1976;72:173–82.CrossrefGoogle Scholar

  • 32.

    Santana O, Andres MF, Sanz J, Errahmani N, Abdeslam L, Gonzalez-Coloma A. Valorization of essential oils from Moroccan aromatic plants. Nat Prod Commun 2014;9:1109–14.PubMedGoogle Scholar

  • 33.

    Judzentiene A, Budiene J. Compositional variation in essential oils of wild Artemisia absinthium from Lithuania. J Essent Oil Bear Pl 2010;13:275–85.CrossrefGoogle Scholar

  • 34.

    Kordali S, Kotan R, Mavi A, Cakir A, Ala A, Yildirim A. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. J Agric Food Chem 2005;53:9452–8.Google Scholar

  • 35.

    Blagojevic P, Radulovic N, Palic R, Stojanovic G. Chemical composition of the essential oils of Serbian wild-growing Artemisia absinthium and Artemisia vulgaris. J Agric Food Chem 2006;54:4780–9.PubMedCrossrefGoogle Scholar

  • 36.

    Ferreira JF, Peaden P, Keiser J. In vitro trematocidal effects of crude alcoholic extracts of Artemisia annua, A. absinthium, Asimina triloba, and Fumaria officinalis: trematocidal plant alcoholic extracts. Parasitol Res 2011;109:1585–92.CrossrefWeb of ScienceGoogle Scholar

  • 37.

    Caner A, Doskaya M, Degirmenci A, Can H, Baykan S, Uner A, et al. Comparison of the effects of Artemisia vulgaris and Artemisia absinthium growing in western Anatolia against trichinellosis (Trichinella spiralis) in rats. Exp Parasitol 2008;119:173–9.Google Scholar

  • 38.

    Dias CR, Schwan AV, Ezequiel DP, Sarmento MC, Ferraz S. Efeito de extractos aquosos de plantas medicinais na sobrevivencia de juvenis de Meloidogyne incognita. Nematol Bras 2000;24:203–10.Google Scholar

  • 39.

    Barbosa P, Lima AS, Vieira P, Dias LS, Tinoco MT, Barroso JG. Nematocidal activity of essential oils and volatiles derived from Portuguese aromatic flora against the pinewood nematode, Bursaphelenchus xylophilus. J Nematol 2010;42:8–16.Google Scholar

  • 40.

    Zouhar M, Douda O, Lhotsky D, Pavela R. Effect of plant essential oils on mortality of the stem nematode (Ditylenchus dipsaci). Plant Prot Sci 2009;45:66–73.Google Scholar

  • 41.

    Davies KG, Curtis RH. Cuticle surface coat of plant-parasitic nematodes. Annu Rev Phytopathol 2011;49:135–56.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 42.

    López de Mendoza ME, Curtis RH, Gowen SR. Identification and characterization of excreted-secreted products and surface coat antigens of animal and plant-parasitic nematodes. Parasitology 1999;118:397–405.CrossrefGoogle Scholar

  • 43.

    López-Arellano ME. Immunomolocalization of Trichinella spiralis L1 surece and excreted/secreted antigens in situ. Int J Nematol 2002;12:55–8.Google Scholar

  • 44.

    López de Mendoza ME, Abrantes IM, Rowe J, Curtis RH. Immunolocalisation in plants of secretions form parasitic stages of Meloidogyne incognita and M. hispanica. Int J Nematol 2002;12:149–54.Google Scholar

  • 45.

    Blaxter M, Dorris M, De Ley P. Patterns and processes in the evolution of animal parasitic nematodes. Nematology 2000;2:243–55.CrossrefGoogle Scholar

  • 46.

    Despommier DD. Trichinella spiralis and the concept of niche. J Parasitol 1993;79:472–82.CrossrefPubMedGoogle Scholar

  • 47.

    Abad P, Favery B, Rosso MN, Castagnone-Sereno P. Root-knot nematode parasitism and host response: molecular basis of a sophisticated interaction. Mol Plant Pathol 2003;4:217–24.CrossrefPubMedGoogle Scholar

About the article

Corresponding author: Juan José García-Rodríguez, CEI Campus Moncloa (UCM-UPM and CSIC), Madrid, Spain; and Facultad de Farmacia, Departamento de Parasitología, Universidad Complutense de Madrid, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain, Phone: +34 91 394 18 16, Fax: +34 91 394 18 17, E-mail:

Received: 2015-04-10

Revised: 2015-09-02

Accepted: 2015-09-02

Published Online: 2015-10-07

Published in Print: 2015-09-01

Citation Information: Zeitschrift für Naturforschung C, Volume 70, Issue 9-10, Pages 275–280, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2015-0109.

Export Citation

©2015 by De Gruyter.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Ma Rosa Pino-Otín, Diego Ballestero, Enrique Navarro, Azucena González-Coloma, Jonatan Val, and Ana M. Mainar
Chemosphere, 2018
Mohammad Ali, Bilal Haider Abbasi, Nisar Ahmad, Haji Khan, and Gul Shad Ali
Critical Reviews in Biotechnology, 2017, Volume 37, Number 7, Page 833

Comments (0)

Please log in or register to comment.
Log in