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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

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1865-7125
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Volume 73, Issue 3-4

Issues

Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici

Amena Khatun
  • Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
  • Other articles by this author:
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/ Tarin Farhana
  • Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
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/ Abdullah As Sabir
  • Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
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/ Shah Mohammad Naimul Islam
  • Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
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/ Helen M. West / Mahfuzur Rahman / Tofazzal Islam
  • Corresponding author
  • Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
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Published Online: 2018-02-03 | DOI: https://doi.org/10.1515/znc-2017-0065

Abstract

The objective of this study was to isolate and characterize antagonistic rhizobacteria from chili against a notorious phytopathogen Phytophthora capsici. Among the 48 bacteria isolated, BTLbbc-02, BTLbbc-03, and BTLbbc-05 were selected based on their inhibitory activity against P. capsici. They were tentatively identified as Burkholderia metallica BTLbbc-02, Burkholderia cepacia BTLbbc-03, and Pseudomonas aeruginosa BTLbbc-05, respectively, based on their 16S rRNA gene sequencing. All inhibited the growth of P. capsici at varying levels by inducing characteristic morphological alterations of P. capsici hyphae. The cell-free culture supernatant of all three isolates impaired motility (up to 100%) and caused lysis (up to 50%) of the halted zoospores. Bioassays revealed that Pseudomonas sp. had higher antagonism and zoospore motility-inhibitory effects against P. capsici compared with two other isolates, Burkholderia spp. and B. metallica, which caused vacuolation in mycelium. All three bacteria suppressed sporangium formation and zoosporogenesis of P. capsici, and improved the seed germination and growth of cucumber. Our findings suggest that epiphytic bacteria, B. metallica, B. cepacia, and P. aeruginosa, could be used as potential biocontrol agents against P. capsici. A further study is required to ensure conformity with the existing regulations for soil, plant, and human health.

This article offers supplementary material which is provided at the end of the article.

Keywords: biocontrol; biopesticide; Phytophthora; rhizobacteria; zoospore

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About the article

Received: 2017-04-16

Revised: 2017-10-25

Accepted: 2017-12-18

Published Online: 2018-02-03

Published in Print: 2018-02-23


Competing interests: The authors have declared that there are no conflicts of interests that could be observed as influencing the impartiality of this paper.

Author contributions: A.K. performed experiments, analyzed data, and drafted the manuscript. T.F. and A.A.S performed data collection. T.I., S.M.N.I., M.R., and H.M.W. supervised the design of the study and data analysis, and revised the manuscript. All authors critically reviewed the manuscript for intellectual content and gave final approval for the version to be published.


Citation Information: Zeitschrift für Naturforschung C, Volume 73, Issue 3-4, Pages 123–135, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2017-0065.

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