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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

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Volume 73, Issue 5-6


Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi

Christèle Sorèle Mambou
  • Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon
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/ Raymond Ngansop Nono
  • Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon
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/ Jean Rodolphe Chouna
  • Corresponding author
  • Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon, Phone: 00 237 673551059
  • Email
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/ Jean-de-Dieu Tamokou
  • Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon
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/ Pépin Nkeng-Efouet-Alango
  • Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon
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/ Norbert Sewald
  • Organic and Bioorganic Chemistry, Department of Chemistry, Bielefeld University, P.O. Box 100131, 33501, Bielefeld, Germany
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Published Online: 2018-03-28 | DOI: https://doi.org/10.1515/znc-2017-0207


The antibacterial-guided investigation of the stem bark extract of Pseudocedrela kotschyi led to the isolation of a new secotirucallane triterpene derivative: 4-hydroxy-3,4-secotirucalla-7,24-dien-3,21-dioic acid (1), together with the known one: 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic acid (2) and 3-methyl ester 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic (3). The structures of the isolated compounds were elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy. Extracts, fractions and compounds (1–3) were tested in vitro for antibacterial activity against two Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus ATCC 25923), and two Gram negative bacteria (Escherichia coli S2(1) and Pseudomonas aeruginosa). The MeOH extract and the Hex/CH2Cl2 (70:30) fraction showed significant levels of activity (MIC=64– 256 μg/mL) compared with the two reference drugs [ciprofloxacin: MIC (0.5–1 μg/mL) and amoxicillin: MIC (1–128 μg/mL)]. Moreover, the compound 2 isolated from this Hex/CH2Cl2 (70:30) fraction had the greatest potential value against S. aureus, E. coli and P. aeruginosa, with minimum inhibitory concentrations (MIC) ranging from 4–16 μg/mL.

This article offers supplementary material which is provided at the end of the article.

Keywords: antibacterial; Meliaceae; Pseudocedrela kotschyi; secotirucallane triterpenes


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About the article

Received: 2017-11-11

Revised: 2018-01-12

Accepted: 2018-01-20

Published Online: 2018-03-28

Published in Print: 2018-04-25

Citation Information: Zeitschrift für Naturforschung C, Volume 73, Issue 5-6, Pages 241–246, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2017-0207.

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