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Zeitschrift für Naturforschung C

A Journal of Biosciences

Editor-in-Chief: Seibel, Jürgen

Editorial Board: Aigner , Achim / Boland, Wilhelm / Bornscheuer, Uwe / Hoffmann, Klaus

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5-year IMPACT FACTOR: 0.912

CiteScore 2017: 0.92

SCImago Journal Rank (SJR) 2017: 0.288
Source Normalized Impact per Paper (SNIP) 2017: 0.448

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Volume 71, Issue 7-8


Neem tree (Azadirachta indica) extract specifically suppresses the growth of tumors in H22-bearing Kunming mice

Zhenxiang He
  • Suzhou High-Tech Institute of Nanjing University, Suzhou 215123, China
  • State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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/ Cuihua Jiang
  • Laboratory of Translational Medicine, Jiangsu Academy of Traditional Chinese Medicine, Nanjing 210028, China
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/ Jian Zhang
  • Laboratory of Translational Medicine, Jiangsu Academy of Traditional Chinese Medicine, Nanjing 210028, China
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/ Zhiqi Yin
  • Laboratory of Pharmaceutical Chemistry, China Pharmaceutical University, Nanjing 210009, China
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/ Zengfang Yin
  • College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
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/ Yunfeng Zhu
  • Corresponding author
  • The college of life sciences and bioengineering in Beijing Jiaotong University, Beijing 100044, China
  • The cancer center in Chinese PLA General Hospital, Beijing 100853, China
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/ Jie Fu
  • Corresponding author
  • School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
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Published Online: 2016-06-01 | DOI: https://doi.org/10.1515/znc-2014-4210


Recently, neem tree (Azadirachta indica) extract (NTE) has been reported to have various antitumor activities against gastric, breast, prostate, and skin cancer, respectively. The current study was designed to evaluate the effect of NTE on hepatic cancer in a mouse model. The possible side effects elicited by NTE were also evaluated. The components in NTE were analyzed by liquid chromatography–mass spectrometry (LC-MS). H22 cells-bearing Kumming mice were generated by injecting H22 cells subcutaneously into the right forelimb armpit of the mice. Then the mice were treated daily for 27 days with NTE (150, 300, and 600 mg/kg body weight) by intragastric administration, using carboxymethyl cellulose (CMC, 1%) as blank control and cyclophosphamide (CTX, 20 mg/kg) as positive control. The antitumor effect of NTE was evaluated by assessment of survival rate, body weight, tumor volume and weight, tumor histology, thymus and spleen indexes, and liver histology. The tumor weight and volume in groups of NTE and CTX were significantly lower than those in the CMC group. The survival rate in the NTE group receiving the high dose (600 mg/kg) was significantly higher than that in the CTX and CMC groups. Compared with CTX, NTE was observed to have a tumor-specific cytotoxicity without impairing the normal liver tissue. Additionally, the higher indexes of thymus and spleen indicated that NTE could facilitate the growth of immune organs. The results indicate that NTE is a promising candidate for the antitumor treatment with high efficacy and safety.

This article offers supplementary material which is provided at the end of the article.

Keywords: Azadirachta indica; cyclophosphamide; H22-bearing mice; hepatic cancer


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About the article

Received: 2014-12-05

Revised: 2016-05-02

Accepted: 2016-05-02

Published Online: 2016-06-01

Published in Print: 2016-07-01

Citation Information: Zeitschrift für Naturforschung C, Volume 71, Issue 7-8, Pages 201–208, ISSN (Online) 1865-7125, ISSN (Print) 0939-5075, DOI: https://doi.org/10.1515/znc-2014-4210.

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