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Zeitschrift für Physikalische Chemie

International journal of research in physical chemistry and chemical physics

Editor-in-Chief: Rademann, Klaus

IMPACT FACTOR 2017: 1.144
5-year IMPACT FACTOR: 1.144

CiteScore 2017: 1.08

SCImago Journal Rank (SJR) 2017: 0.495
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Volume 227, Issue 2-3


Second Osmotic Virial Coefficients and Aggregation of Monoclonal Antibodies by Static Laser Light Scattering

Michael Dieterle / Tim Blaschke
  • University of Kaiserslautern, Laboratory of Engineering Thermodynamics (LTD), Kaiserslautern, Deutschland
  • Other articles by this author:
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/ Hans Hasse
Published Online: 2013-01-28 | DOI: https://doi.org/10.1524/zpch.2013.0325


The second osmotic virial coefficient and the apparent molar mass of two human and one mouse monoclonal antibodies were measured in different aequeous buffer solutions which also contained sodium chloride or ammonium sulfate, respectively, by static laser light scattering in batch mode. The apparent molar mass indicates aggregation. At a constant pH value of 6.5 the sodium chloride concentration was varied from 0 to 2 M and the ammonium sulfate concentration from 0 to 0.8 M, respectively. A 20 mM sodium-phosphate buffer was used for all experiments. Furthermore the pH value was varied without adding additional salt from 4.5 to 10. The results of the salt dependency are in line with the Hofmeister-series. The results of the pH dependency correspond to the net charge of the molecules.

Keywords: Protein-Protein Interactions; Second Osmotic Virial Coefficient; Monoclonal Antibody; Molar Mass; Aggregation

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* Correspondence address: University of Kaiserslautern, Laboratory of Engineering Thermodynamics (LTD), Erwin-Schrdinger-Str. 44, 67663 Kaiserslautern, Deutschland,

Published Online: 2013-01-28

Published in Print: 2013-03-01

Citation Information: Zeitschrift für Physikalische Chemie, Volume 227, Issue 2-3, Pages 333–344, ISSN (Online) 2196-7156, ISSN (Print) 0942-9352, DOI: https://doi.org/10.1524/zpch.2013.0325.

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