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Zeitschrift für Physikalische Chemie

International journal of research in physical chemistry and chemical physics

Ed. by Rademann, Klaus

12 Issues per year

IMPACT FACTOR 2016: 1.012

CiteScore 2016: 0.99

SCImago Journal Rank (SJR) 2016: 0.463
Source Normalized Impact per Paper (SNIP) 2016: 0.470

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Volume 227, Issue 2-3 (Mar 2013)


Second Osmotic Virial Coefficients and Aggregation of Monoclonal Antibodies by Static Laser Light Scattering

Michael Dieterle / Tim Blaschke
  • University of Kaiserslautern, Laboratory of Engineering Thermodynamics (LTD), Kaiserslautern, Deutschland
  • Other articles by this author:
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/ Hans Hasse
Published Online: 2013-01-28 | DOI: https://doi.org/10.1524/zpch.2013.0325


The second osmotic virial coefficient and the apparent molar mass of two human and one mouse monoclonal antibodies were measured in different aequeous buffer solutions which also contained sodium chloride or ammonium sulfate, respectively, by static laser light scattering in batch mode. The apparent molar mass indicates aggregation. At a constant pH value of 6.5 the sodium chloride concentration was varied from 0 to 2 M and the ammonium sulfate concentration from 0 to 0.8 M, respectively. A 20 mM sodium-phosphate buffer was used for all experiments. Furthermore the pH value was varied without adding additional salt from 4.5 to 10. The results of the salt dependency are in line with the Hofmeister-series. The results of the pH dependency correspond to the net charge of the molecules.

Keywords: Protein-Protein Interactions; Second Osmotic Virial Coefficient; Monoclonal Antibody; Molar Mass; Aggregation

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* Correspondence address: University of Kaiserslautern, Laboratory of Engineering Thermodynamics (LTD), Erwin-Schrdinger-Str. 44, 67663 Kaiserslautern, Deutschland,

Published Online: 2013-01-28

Published in Print: 2013-03-01

Citation Information: Zeitschrift für Physikalische Chemie, ISSN (Online) 2196-7156, ISSN (Print) 0942-9352, DOI: https://doi.org/10.1524/zpch.2013.0325.

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