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Zeitschrift für Physikalische Chemie

International journal of research in physical chemistry and chemical physics

Editor-in-Chief: Rademann, Klaus


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Volume 231, Issue 2

Issues

Effect of Dehydrated Trehalose Matrix on the Kinetics of Forward Electron Transfer Reactions in Photosystem I

Ivan Shelaev
  • N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russian Federation
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/ Michael Gorka
  • Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, United States of America
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/ Anton Savitsky
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  • Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470 Mülheim an der Ruhr, Germany, Phone: 0049-208-3063555, Fax: 0049-208-3063955
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/ Vasily Kurashov
  • Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, United States of America
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/ Mahir Mamedov
  • A.N. Belozersky Institute of Physical–Chemical Biology, Moscow State University, Moscow, Leninskie Gory, Moscow 119992, Russian Federation
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/ Fedor Gostev
  • N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russian Federation
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/ Klaus Möbius
  • Corresponding author
  • Max-Planck-Institut für Chemische Energiekonversion, Stiftstr. 34-36, D-45470 Mülheim an der Ruhr, Germany
  • Fachbereich Physik, Freie Universität Berlin, Arnimallee 14, D-14195 Berlin, Germany
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/ Victor Nadtochenko
  • Corresponding author
  • N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russian Federation
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/ John Golbeck
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  • Department of Biochemistry and Molecular Biology, Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, United States of America
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/ Alexey Semenov
  • Corresponding author
  • N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119991 Moscow, Russian Federation
  • A.N. Belozersky Institute of Physical–Chemical Biology, Moscow State University, Moscow, Leninskie Gory, Moscow 119992, Russian Federation
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Published Online: 2016-11-03 | DOI: https://doi.org/10.1515/zpch-2016-0860

Abstract

The effect of dehydration on the kinetics of forward electron transfer (ET) has been studied in cyanobacterial photosystem I (PS I) complexes in a trehalose glassy matrix by time-resolved optical and EPR spectroscopies in the 100 fs to 1 ms time domain. The kinetics of the flash-induced absorption changes in the subnanosecond time domain due to primary and secondary charge separation steps were monitored by pump–probe laser spectroscopy with 20-fs low-energy pump pulses centered at 720 nm. The back-reaction kinetics of P700 were measured by high-field time-resolved EPR spectroscopy and the forward kinetics of A1A/A1BFX by time-resolved optical spectroscopy at 480 nm. The kinetics of the primary ET reactions to form the primary P700+A0 and the secondary P700+A1 ion radical pairs were not affected by dehydration in the trehalose matrix, while the yield of the P700+A1 was decreased by ~20%. Forward ET from the phylloquinone molecules in the A1A and A1B sites to the iron–sulfur cluster FX slowed from ~220 ns and ~20 ns in solution to ~13 μs and ~80 ns, respectively. However, as shown by EPR spectroscopy, the ~15 μs kinetic phase also contains a small contribution from the recombination between A1B and P700+. These data reveal that the initial ET reactions from P700 to secondary phylloquinone acceptors in the A- and B-branches of cofactors (A1A and A1B) remain unaffected whereas ET beyond A1A and A1B is slowed or prevented by constrained protein dynamics due to the dry trehalose glass matrix.

Keywords: electron transfer; EPR; optical spectroscopy; photosystem I; trehalose matrix

Dedicated to: Kev Salikhov on the occasion of his 80th birthday.

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About the article

Received: 2016-07-18

Accepted: 2016-10-05

Published Online: 2016-11-03

Published in Print: 2017-02-01


Funding Source: Russian Foundation for Basic Research

Award identifier / Grant number: 15-04-04252

Funding Source: Russian Science Foundation

Award identifier / Grant number: 14-14-00789

This work was financially supported by the Russian Foundation for Basic Research, grant 15-04-04252 (to A.Yu.S.) and Russian Science Foundation, grant 14-14-00789 (to A.Yu.S. and V.N.), by the President of RF grant MK-6515.2015.4 (to I.S.), by the US National Science Foundation, grant MCB-1613022 (to J.G.) and by the Cluster of Excellence RESOLV (EXC 1069) funded by the Deutsche Forschungsgemeinschaft (to A.S.) and the Max Planck Society (to A.S. and K.M.). K.M. acknowledges sustaining support by the Free University of Berlin. We want to thank Giovanni Venturoli (University of Bologna) for stimulating and fruitful discussions, Dmitry Cherepanov and Georgy Milanovsky for their assistance in application of CONTIN software, and Anastasia Petrova for preparation of PS I complexes (all Moscow State University).


Citation Information: Zeitschrift für Physikalische Chemie, Volume 231, Issue 2, Pages 325–345, ISSN (Online) 2196-7156, ISSN (Print) 0942-9352, DOI: https://doi.org/10.1515/zpch-2016-0860.

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