Heterocotyle tokoloshei sp. nov. (Monogenea, Monocotylidae) from the gills of Dasyatis brevicaudata (Dasyatidae) kept in captivity at Two Oceans Aquarium, Cape Town, South Africa: Description and notes on treatment

David Vaughan 1  and Leslie Chisholm
  • 1 Aquatic Animal Health Research, Two Oceans Aquarium, P.O. Box 50603, Victoria and Alfred Waterfront, Cape Town, 8000, South Africa
  • 2 Monogenean Research Laboratory, Parasitology Section, The South Australian Museum, North Terrace, Adelaide, South Australia, 5000, Australia
  • 3 Marine Parasitology Laboratory, School of Earth and Environmental Sciences (DX 650 418), The University of Adelaide, North Terrace, Adelaide, South Australia, 5005, Australia

Abstract

Heterocotyle tokoloshei sp. nov. is described from the gills of a single Short-tail stingray, Dasyatis brevicaudata, kept in captivity at the Two Oceans Aquarium in Cape Town, South Africa. The stingray exhibited laboured gill ventilation and deteriorating health on exhibit and was removed to the quarantine area for parasitological study and treatment. A 12 h bath treatment of praziquantel at 20mg/l, pre-dissolved in ethanol, removed 3084 parasites from the gills of the ray. However, the presence of a large number of eggs 24 h post-treatment indicated that viable egg laying adults remained on the gills and that the treatment was not 100% effective. Praziquantel was subsequently administered orally by intubation to the same ray at 150 mg/kg under anaesthetic (2-phenoxyethanol at 0.15 ml/l for approximately 1 h), which resulted in the removal of approximately 392 000 parasites from the gills 12 h post-oral treatment. Twenty-four h post-oral treatment, 3383 worms, but no eggs were recovered. No worms or eggs were recovered 48 h to 10 days post-oral treatment. The ray died approximately 30 days after the completion of the treatment. Heterocotyle tokoloshei sp. nov. is the first Heterocotyle species described from South Africa and represents the first record of a pathogenic Heterocotyle species. The new species can be distinguished from the other 16 species in the genus by the distal region of the male copulatory organ which has distinct small spines and by the morphology of the male copulatory organ accessory piece. Eggs of H. tokoloshei sp. nov. are laid singly and hatch spontaneously between 5 and 8 days at 18°C.

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