Lysosomal proteinases are translated as preproenzymes,
transferred through the Golgi apparatus as
proenzymes, and localized in lysosomes as the mature
enzymes. Pulsechase analyses and the immunoisolation
of proenzymes or recombinant proenzymes
are useful tools for analyzing this process, but
the processing proteinases that participate in this
pathway are largely unknown. Recently, we developed
a new method for analyzing processing proteinases
using Bafilomycin A1 and proteinase inhibitors.
Here we summarize the recent progress
including our results obtained using this method.
Biological Chemistry keeps you up-to-date with the latest advances in the molecular life sciences. The journal publishes Research Articles, Short Communications, Reviews and Minireviews. Areas include: general biochemistry/pathobiochemistry, structural biology, molecular and cellular biology, genetics and epigenetics, virology, molecular medicine, plant molecular biology/biochemistry and novel experimental methodologies.