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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Processing and Activation of Lysosomal Proteinases

  • K. Ishidoh and E. Kominami
From the journal Biological Chemistry

Abstract

Lysosomal proteinases are translated as preproenzymes, transferred through the Golgi apparatus as proenzymes, and localized in lysosomes as the mature enzymes. Pulsechase analyses and the immunoisolation of proenzymes or recombinant proenzymes are useful tools for analyzing this process, but the processing proteinases that participate in this pathway are largely unknown. Recently, we developed a new method for analyzing processing proteinases using Bafilomycin A1 and proteinase inhibitors. Here we summarize the recent progress including our results obtained using this method.

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Published Online: 2005-06-01
Published in Print: 2002-12-09

Copyright © 2002 by Walter de Gruyter GmbH & Co. KG

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