The zinc finger protein ZNF297B interacts with BDP1, a subunit of TFIIIB

Frank Schoenen 1.  and Brunhilde Wirth 2.
  • 1. Institute of Human Genetics, University of Cologne, D-50674 Cologne, Germany and Institute of Genetics, University of Cologne, D-50674 Cologne, Germany
  • 2. Institute of Human Genetics, University of Cologne, D-50674 Cologne, Germany, Institute of Genetics, University of Cologne, D-50674 Cologne, Germany and Center for Molecular Medicine Cologne, University of Cologne, D-50931 Cologne, Germany

Abstract

The human gene BDP1, localized on chromosome 5q13 in close proximity to the spinal muscular atrophy determining gene SMN, encodes a large protein consisting of 2254 amino acids (aa). In the first third of the gene, the subunit of the RNA polymerase III (Pol III) transcription factor complex (TFIIIBα/β) is encoded. To further characterize the function of BDP1, we carried out a yeast two-hybrid screen using various parts of BDP1. With the clone BDP1-(1–640) we identified a novel interaction partner, ZNF297B. The ZNF297B gene is localized on chromosome 9q24 and encodes a zinc finger protein of 467 aa possessing the typical structure of a transcription factor. The interaction found in yeast was confirmed by co-immunoprecipitation and refined to the N-terminal region of ZNF297B-(1–127) containing the BTB/POZ domain and the N-terminal end of BDP1-(1–299). The ZNF297B transcript is 5.7 kb in length and ubiquitously expressed, with highest levels found in muscles. Immunofluorescence staining revealed a speckled pattern in the nuclei of HEK293 cells. Due to the essential role of BDP1 in Pol III transcription, we propose that ZNF297B may also regulate these transcriptional pathways.

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