Idiosyncratic cleavage and ligation activity of individual hammerhead ribozymes and core sequence variants thereof

Rita Przybilski 1 , 1  and Christian Hammann 2 , 2
  • 1 AG Molecular Interactions, Department of Genetics, University of Kassel, Heinrich-Plett-Str. 40, D-34132 Kassel, Germany
  • 2 AG Molecular Interactions, Department of Genetics, University of Kassel, Heinrich-Plett-Str. 40, D-34132 Kassel, Germany

Abstract

The hammerhead ribozyme is a small RNA endonuclease found in sub-viral plant pathogens, in transcripts from certain animal satellite DNAs and encoded at distinct loci of Arabidopsis thaliana. Kinetic analyses of tertiary stabilised ribozymes from peach latent mosaic viroid (PLMVd), Schistosoma mansoni and A. thaliana revealed a ten-fold difference in cleavage rates. Core nucleotide variations affected cleavage reactions least in the A. thaliana ribozyme, and most in the S. mansoni ribozyme. The reverse ligation reaction was catalysed efficiently by the PLMVd and A. thaliana ribozymes. The different behaviour of the individual hammerhead ribozymes is discussed in terms of structure and function.

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Biological Chemistry keeps you up-to-date with the latest advances in the molecular life sciences. The journal publishes Research Articles, Short Communications, Reviews and Minireviews. Areas include: general biochemistry/pathobiochemistry, structural biology, molecular and cellular biology, genetics and epigenetics, virology, molecular medicine, plant molecular biology/biochemistry and novel experimental methodologies.

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