PqsA is required for the biosynthesis of 2,4-dihydroxyquinoline (DHQ), a newly identified metabolite produced by Pseudomonas aeruginosa and Burkholderia thailandensis

François Lépine 1 , Valérie Dekimpe 2 , Biliana Lesic 3 , Sylvain Milot 4 , Alain Lesimple 5 , Orval A. Mamer 6 , Laurence G. Rahme 7 , and Eric Déziel 8
  • 1 INRS-Institut Armand-Frappier, Laval H7V 1B7, Québec, Canada
  • 2 INRS-Institut Armand-Frappier, Laval H7V 1B7, Québec, Canada
  • 3 Department of Surgery, Harvard Medical School, Massachusetts General Hospital, and Shriner's Burns Institute, Boston, MA 02114, USA
  • 4 INRS-Institut Armand-Frappier, Laval H7V 1B7, Québec, Canada
  • 5 McGill Biomedical Mass Spectrometry Unit, McGill University, Montreal H3A 1A4, Quebec, Canada
  • 6 McGill Biomedical Mass Spectrometry Unit, McGill University, Montreal H3A 1A4, Quebec, Canada
  • 7 Department of Surgery, Harvard Medical School, Massachusetts General Hospital, and Shriner's Burns Institute, Boston, MA 02114, USA
  • 8 INRS-Institut Armand-Frappier, Laval H7V 1B7, Québec, Canada

Abstract

A new metabolite, 2,4-dihydroxyquinoline (DHQ), was identified in cultures of the bacteria Pseudomonas aeruginosa and Burkholderia thailandensis. We found that the biosynthesis of DHQ correlates with the presence of a functional PqsA, which is a product of the pqsABCDE operon responsible for the synthesis of 4-hydroxy-2-alkylquinolines (HAQs) in P. aeruginosa. However, DHQ is not a degradation product or precursor of HAQs. This finding sheds some light on the poorly understood biosynthesis pathway of HAQs, which includes important communication signals regulating the expression of virulence factors.

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