The purpose of this project was to investigate
the angiogenic mechanism of bioactive borate glass for
soft tissue repair in a ‘hairless’ SKH1 mouse model. Subcutaneous
microvascular responses to bioactive glass microfibers
(45S5, 13-93B3, and 13-93B3Cu) and bioactive
glass beads (13-93, 13-93B3, and 13-93B3Cu) were assessed
via: noninvasive imaging of skin microvasculature; histomorphometry
of microvascular densities; and quantitative
PCR measurements of mRNA expression of VEGF
and FGF-2 cytokines. Live imaging via dorsal skin windows
showed the formation at twoweeks of a halo-like structure
infused with microvessels surrounding implanted boratebased
13-93B3 and 13-93B3Cu glass beads, a response not
observed with silicate-based 13-93 glass beads. Quantitative
histomorphometry of tissues implanted with plugs of
45S5, 13-93B3, and 13-93B3Cu glass microfibers revealed
microvascular densities that were 1.6-, 2.3-, and 2.7-times
higher, respectively, than the sham control valueswhereas
13-93, 13-93B3, and 13-93B3Cu glass beads caused the microvascular
density to increase 1.3-, 1.6-, and 2.5-fold,
respectively, relative to sham controls. Quantitative PCR
measurements indicate a marginally significant increased
expression of VEGF mRNA in tissues with 13-93B3Cu glass
beads, an outcome that supported the hypothesis that
copper-doped borate glass could promote VEGF expression
followed by angiogenesis for enhanced wound healing.
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Biomedical Glasses is an international open access journal covering the field of glasses for biomedical applications. The aim of the journal is to provide a peer-reviewed forum for the publication of original research reports and authoritative review articles related to the development of biomedical glasses and their use in clinical applications.