Effects of combining Beauveria bassiana and Nosema pyrausta on the mortality of Ostrinia nubilalis

Khaled Abdel Rahman 1 , Marek Barta 2 , and Ľudovít Cagáň 3
  • 1 Department of Entomology, Faculty of Sciences, Cairo University, 12613, Giza, Egypt
  • 2 Department of Dendrobiology, Arboretum Mlynany SAS, 951 52, Slepcany, Slovak Republic
  • 3 Department of Plant Protection, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, 949 76, Nitra, Slovak Republic

Abstract

We tested the combined effect of the fungus Beauveria bassiana and the microsporidium Nosema pyrausta on the European corn borer larvae, Ostrinia nubilalis, in the laboratory. The first instar of O. nubilalis larvae was the most sensitive to the B. bassiana infection followed by the fifth, second, third, and fourth instar (LC50s were 4.91, 6.67, 7.13, 9.15, and 6.51 × 105 conidia/ml for the first to fifth instars, respectively). Mortality of each instar increases positively with concentration of conidia. When B. bassiana and N. pyrausta were used in combination, mortality increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16%, 37.63%, 21.60%, 27.11%, and 33.95% for the first to fifth instars, respectively. The combined effects of the two pathogens were mostly additive. However, at the two highest concentrations the pathogens interacted synergistically in the first and second instar. Individuals that survived the B. bassiana and B. bassiana + N. pyrausta treatments and developed into adults had significantly shorter lifespans and females oviposited fewer eggs than non-exposed insects. The effects on the longevity and the egg production were most pronounced at high concentration of B. bassiana conidia.

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