Endoxy – development and cultivation of textile-based gas membrane assemblies for endothelialized oxygenators

Christine Neusser, Nicole Finocchiaro 2 , Felix Hesselmann 3 , Christian Cornelissen, Thomas Gries 1 , and Stefan Jockenhoevel
  • 1 Institut für Textiltechnik Aachen at RWTH Aachen University – Department of Tissue Engineering and Textile Implants, Otto-Blumenthal-Str. 1, 52074 Aachen, Germany
  • 2 Institute of Applied Medical Engineering – Department of Tissue Engineering and Textile Implants, Helmholtz Institute of RWTH Aachen University and Hospital, Pauwelsstr. 20, 52074 Aachen, Germany
  • 3 Institute of Applied Medical Engineering – Department of Cardiovascular Engineering, Helmholtz Institute of RWTH Aachen University and Hospital, Pauwelsstr. 20, 52074 Aachen, Germany
  • 4 Department for Internal Medicine – Section for Pneumology, University Hospital Aachen, Pauwelsstr. 30, 52074 Aachen, Germany
Christine Neusser, Nicole Finocchiaro, Felix Hesselmann, Christian Cornelissen, Thomas Gries and Stefan Jockenhoevel

Abstract

One step to enable long-term use of extracorporeal membrane oxygenation devices or even the development of an artificial fully implantable lung is the endothelialization of oxygenator membranes in order to present a physiological and anti-thrombogenic surface to the blood flow. Since cell seeding decreases the gas transfer of oxygenation devices, a way to reincrease gas exchange performance by other means has to be found. In this study membrane assemblies suitable for endothelialization were developed, which profit from a secondary flow arrangement to increase gas transfer rates, the so-called Bellhouse effect. Therefore textiles that allow a homogeneous gas flow between the membranes and provide a structure that can be used as mold for hollow imprinting onto the membrane surfaces are combined with flat membranes to a sandwich structure. On top of that two approaches for hollow imprinting are generated and their results compared. The furrowed membrane assemblies are seeded with HUVECs and regularly inspected over 3 days cultivation. A surface characterization of the applied membranes is performed by contact angle measurement to identify reasons for inhomogeneous cell growth. In general first important results to develop a biohybrid lung assist device could be achieved in this study.

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