Effect of surface modification of zirconia on cell adhesion, metabolic activity and proliferation of human osteoblasts

Waleed M.S. Al Qahtani, Christine Schille, Sebastian Spintzyk, Mohammed S.A. Al Qahtani, Eva Engel, Juergen Geis-Gerstorfer, Frank Rupp and Lutz Scheideler

Abstract

Titanium dental implants with sandblasted and/or acid-etched surfaces have shown clinical superiority in comparison to their smooth, machined counterparts, and are now state of the art. Sandblasting of finished, sintered zirconia implants, however, will damage the surface structure and affect the mechanical properties. To improve osseointegration of zirconia dental implants without impairing the original mechanical strength by crack initiation and partial phase transformation from tetragonal to monoclinic, roughening of the zirconia surface by sandblasting before the final sintering step was employed. Impact of the treatments on cellular reactions of SAOS-2 human osteoblast-like cells was investigated. Sandblasting of Yttrium-stabilized zirconia (Y-TZP) with 120 μm and 250 μm Al2O3 enhanced average roughness (Sa) from 0.28 μm to 4.1 μm and 5.72 μm, respectively. Cell adhesion of SAOS-2 osteoblasts was enhanced up to 175% on sandblasted surfaces, compared to the machined zirconia reference (100%). Metabolic activity and proliferation in the logarithmic growth phase (24–48 h) were not significantly affected. Sample surface coverage by the cells after prolonged incubation (72 h) was markedly decreased on the roughened samples, indicating a shift towards increased differentiation on these surfaces. The approach investigated here to roughen zirconia implants by sandblasting before sintering shows potential to improve the clinical performance of ceramic dental implants.

  • [1]

    Adell R, Eriksson B, Lekholm U, Branemark PI, Jemt T. Long-term follow-up study of osseointegrated implants in the treatment of totally edentulous jaws. Int J Oral Max Impl 1990; 5: 347–359.

  • [2]

    Albrektsson T, Wennerberg A. Oral implant surfaces: part 1–review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont 2004; 17: 536–543.

  • [3]

    Albrektsson T, Hansson HA, Ivarsson B. Interface analysis of titanium and zirconium bone implants. Biomaterials 1985; 6: 97–101.

  • [4]

    Anderson HC, Sugamoto K, Morris DC, Hsu HH, Hunt T. Bone-inducing agent (BIA) from cultured human Saos-2 osteosarcoma cells. Bone Miner 1992; 16: 49–62.

  • [5]

    Anselme K, Bigerelle M, Noel B, et al. Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses. J Biomed Mater Res 2000; 49: 155–166.

  • [6]

    Borghetti P, De Angelis E, Caldara G, Corradi A, Cacchioli A, Gabbi C. Adaptive response of osteoblasts grown on a titanium surface: morphology, cell proliferation and stress protein synthesis. Vet Res Commun 2005; 29(Suppl 2): 221–224.

  • [7]

    Bowers KT, Keller JC, Randolph BA, Wick DG, Michaels CM. Optimization of surface micromorphology for enhanced osteoblast responses in vitro. Int J Oral Max Impl 1992; 7: 302–310.

  • [8]

    Boyan BD, Batzer R, Kieswetter K, et al. Titanium surface roughness alters responsiveness of MG63 osteoblast-like cells to 1 alpha,25-(OH)2D3. J Biomed Mater Res 1998; 39: 77–85.

  • [9]

    Buser D, Nydegger T, Oxland T, et al. Interface shear strength of titanium implants with a sandblasted and acid-etched surface: a biomechanical study in the maxilla of miniature pigs. J Biomed Mater Res 1999; 45: 75–83.

  • [10]

    Buser D, Schenk RK, Steinemann S, Fiorellini JP, Fox CH, Stich H. Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs. J Biomed Mater Res 1991; 25: 889–902.

  • [11]

    Cooper LF, Masuda T, Whitson SW, Yliheikkila P, Felton DA. Formation of mineralizing osteoblast cultures on machined, titanium oxide grit-blasted, and plasma-sprayed titanium surfaces. Int J Oral Max Impl 1999; 14: 37–47.

  • [12]

    De Wijs FL, Van Dongen RC, De Lange GL, De Putter C. Front tooth replacement with Tubingen (Frialit) implants. J Oral Rehabil 1994; 21: 11–26.

  • [13]

    Degasne I, Basle MF, Demais V, et al. Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2) on titanium surfaces. Calcified Tissue Int 1999; 64: 499–507.

  • [14]

    Gahlert M, Gudehus T, Eichhorn S, Steinhauser E, Kniha H, Erhardt W. Biomechanical and histomorphometric comparison between zirconia implants with varying surface textures and a titanium implant in the maxilla of miniature pigs. Clin Oral Implan Res 2007; 18: 662–668.

  • [15]

    Gahlert M, Roehling S, Sprecher CM, Kniha H, Milz S, Bormann K. In vivo performance of zirconia and titanium implants: a histomorphometric study in mini pig maxillae. Clin Oral Implan Res 2012; 23: 281–286.

  • [16]

    Garcia Fonseca R, de Oliveira Abi-Rached F, dos Santos Nunes Reis JM, Rambaldi E, Baldissara P. Effect of particle size on the flexural strength and phase transformation of an airborne-particle abraded yttria-stabilized tetragonal zirconia polycrystal ceramic. J Prosthet Dent 2013; 110: 510–514.

  • [17]

    Geis-Gerstorfer J, Faessler P. Untersuchungen zum Ermüdungsverhalten der Dentalkeramiken Zirkondioxid-TZP und In-Ceram. Dtsch Zahnarztl Z 1999; 54: 692–694.

  • [18]

    Gittens RA, Scheideler L, Rupp F, et al. A review on the wettability of dental implant surfaces II: biological and clinical aspects. Acta Biomater 2014; 10: 2907–2918.

  • [19]

    Grossner-Schreiber B, Tuan RS. [The influence of the titanium implant surface on the process of osseointegration]. Dtsch Zahnarztl Z 1991; 46: 691–693.

  • [20]

    Guess PC, Zhang Y, Kim JW, Rekow ED, Thompson VP. Damage and reliability of Y-TZP after cementation surface treatment. J Dent Res 2010; 89: 592–596.

  • [21]

    Herrero-Climent M, Lazaro P, Rios JV, et al. Influence of acid-etching after grit-blasted on osseointegration of titanium dental implants: in vitro and in vivo studies. J Mater Sci-Mater M 2013; 24: 2047–2055.

  • [22]

    Heydecke G, Kohal R, Glaser R. Optimal esthetics in single-tooth replacement with the Re-Implant system: a case report. Int J Prosthodont 1999; 12: 184–189.

  • [23]

    Hunt TR, Schwappach JR, Anderson HC. Healing of a segmental defect in the rat femur with use of an extract from a cultured human osteosarcoma cell-line (Saos-2) – A preliminary report. J Bone Joint Surg Am 1996; 78A: 41–48.

  • [24]

    Ichikawa Y, Akagawa Y, Nikai H, Tsuru H. Tissue compatibility and stability of a new zirconia ceramic in vivo. J Prosthet Dent 1992; 68: 322–326.

  • [25]

    Kasemo B, Lausmaa J. Biomaterial and implant surfaces: a surface science approach. Int J Oral Max Impl 1988; 3: 247–259.

  • [26]

    Kelly JR, Denry I. Stabilized zirconia as a structural ceramic: an overview. Dent Mater 2008; 24: 289–298.

  • [27]

    Keselowsky BG, Wang L, Schwartz Z, Garcia AJ, Boyan BD. Integrin alpha(5) controls osteoblastic proliferation and differentiation responses to titanium substrates presenting different roughness characteristics in a roughness independent manner. J Biomed Mater Res A 2007; 80: 700–710.

  • [28]

    Kieswetter K, Schwartz Z, Hummert TW, et al. Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. J Biomed Mater Res 1996; 32: 55–63.

  • [29]

    Kohal RJ, Klaus G. A zirconia implant-crown system: a case report. Int J Periodont Rest 2004; 24: 147–153.

  • [30]

    Kohal RJ, Papavasiliou G, Kamposiora P, Tripodakis A, Strub JR. Three-dimensional computerized stress analysis of commercially pure titanium and yttrium-partially stabilized zirconia implants. Int J Prosthodont 2002; 15: 189–194.

  • [31]

    Kosmac T, Oblak C, Jevnikar P, Funduk N, Marion L. The effect of surface grinding and sandblasting on flexural strength and reliability of Y-TZP zirconia ceramic. Dent Mater 1999; 15: 426–433.

  • [32]

    Larsson C, Thomsen P, Lausmaa J, Rodahl M, Kasemo B, Ericson LE. Bone response to surface modified titanium implants: studies on electropolished implants with different oxide thicknesses and morphology. Biomaterials 1994; 15: 1062–1074.

  • [33]

    Lincks J, Boyan BD, Blanchard CR, et al. Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. Biomaterials 1998; 19: 2219–2232.

  • [34]

    Martin JY, Schwartz Z, Hummert TW, et al. Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). J Biomed Mater Res 1995; 29: 389–401.

  • [35]

    Monaco C, Tucci A, Esposito L, Scotti R. Microstructural changes produced by abrading Y-TZP in presintered and sintered conditions. J Dent 2013; 41: 121–126.

  • [36]

    Mustafa K, Wennerberg A, Wroblewski J, Hultenby K, Lopez BS, Arvidson K. Determining optimal surface roughness of TiO(2) blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone. Clin Oral Implan Res 2001; 12: 515–525.

  • [37]

    Okumura A, Goto M, Goto T, et al. Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2). Biomaterials 2001; 22: 2263–2271.

  • [38]

    Postiglione L, Di Domenico G, Ramaglia L, et al. Behavior of SaOS-2 cells cultured on different titanium surfaces. J Dent Res 2003; 82: 692–696.

  • [39]

    Postiglione L, Di Domenico G, Ramaglia L, di Lauro AE, Di Meglio F, Montagnani S. Different titanium surfaces modulate the bone phenotype of SaOS-2 osteoblast-like cells. Eur J Histochem 2004; 48: 213–222.

  • [40]

    Qeblawi DM, Munoz CA, Brewer JD, Monaco EA, Jr. The effect of zirconia surface treatment on flexural strength and shear bond strength to a resin cement. J Prosthet Dent 2010; 103: 210–220.

  • [41]

    Schulte W, Kleineikenscheidt H, Lindner K, Schareyka R. [The Tubingen immediate implant in clinical studies]. Dtsch Zahnarztl Z 1978; 33: 348–359.

  • [42]

    Sennerby L, Dasmah A, Larsson B, Iverhed M. Bone tissue responses to surface-modified zirconia implants: a histomorphometric and removal torque study in the rabbit. Clin Implant Dent R 2005; 7: S13–S20.

  • [43]

    Silva VV, Lameiras FS, Lobato ZI. Biological reactivity of zirconia-hydroxyapatite composites. J Biomed Mater Res 2002; 63: 583–590.

  • [44]

    Stawarczyk B, Ozcan M, Hallmann L, Roos M, Trottmann A, Hammerle CHF. Impact of air-abrasion on fracture load and failure type of veneered anterior Y-TZP crowns before and after chewing simulation. J Biomed Mater Res B 2012; 100B: 1683–1690.

  • [45]

    Wei N, Bin S, Jing Z, Wei S, Yingqiong Z. Influence of implant surface topography on bone-regenerative potential and mechanical retention in the human maxilla and mandible. American journal of dentistry 2014; 27: 171–176.

  • [46]

    Wennerberg A, Albrektsson T. Effects of titanium surface topography on bone integration: a systematic review. Clin Oral Implan Res 2009; 20: 172–184.

  • [47]

    Zhang Y, Lawn BR, Rekow ED, Thompson VP. Effect of sandblasting on the long-term performance of dental ceramics. J Biomed Mater Res B, Appl Biomater 2004; 71: 381–386.

  • [48]

    Zhang S, Sun J, Xu Y, et al. Biological behavior of osteoblast-like cells on titania and zirconia films deposited by cathodic arc deposition. Biointerphases 2012; 7: 60.

  • [49]

    Zhu X, Chen J, Scheideler L, Altebaeumer T, Geis-Gerstorfer J, Kern D. Cellular reactions of osteoblasts to micron- and submicron-scale porous structures of titanium surfaces. Cells Tissues Organs 2004; 178: 13–22.

  • [50]

    Zhu X, Chen J, Scheideler L, Reichl R, Geis-Gerstorfer J. Effects of topography and composition of titanium surface oxides on osteoblast responses. Biomaterials 2004; 25: 4087–4103.

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