The Fungi are globally distributed members of marine ecosystems (Tisthammer et al. 2016, Morales et al. 2019), whose abundances are tied to phytoplankton (Taylor and Cunliffe 2016), organic matter (Ortega-Arbulú et al. 2018), and elevated photon fluxes (Hassett and Gradinger 2016). Marine fungi have been detected in the sub-seafloor (Orsi et al. 2013), in coastal marine sediments (Picard 2017), throughout the Arctic (Rämä et al. 2017), and cultured extensively in temperate and tropic regions (Jones and Pang 2012a). Marine fungi were known to exist since the 1800s and their diversity has been explored through many vigorous culturing and morphological-based diagnostic studies (Johnson and Sparrow 1961, Kohlmeyer and Kohlmeyer 1979). There are currently between 120,000 and 143,273 accepted fungal species (Hawksworth and Lucking 2017), www.indexfungorum.org); of these, 1255 species have been recovered from the marine realm (Jones et al. 2015, 2019). Even though fungi comprise substantial quantities of biomass in the marine realm (Gutiérrez et al. 2011, Bochdansky et al. 2017, Hassett et al. 2019), their activity is not represented in marine ecosystem models (Worden et al. 2015).
Marine fungi behave as saprobes and symbionts that can recycle nutrients (Gutiérrez et al. 2011). Marine fungi have been reported from a wide range of substrates, such as wood, culms of angiosperms (Posidonia K.D. Koenig, Spartina Schreb.), manmade materials (polyurethane), and as parasites of marine animals (copepods, fish), algae (diatoms, brown seaweeds), corals, and sponges (Chakrarvarity 1974, Pivkin 2000, Lin et al. 2002, Nagai 2002, Proksch et al. 2008, Zheng et al. 2009, 2013, Pang et al. 2011, Jones and Pang 2012b, Debbab et al. 2013, Yao et al. 2014, Gutiérrez et al. 2016, Gnavi et al. 2017, Raghukumar 2017), especially in pursuits of natural product discovery (Bugni and Ireland 2004, Pan et al. 2008, Schulz et al. 2008). Marine fungi have historically been defined as those capable of reaching reproductive maturity while completely or partially inundated with seawater salinity of at least 30 during some point in their life cycle (Johnson and Sparrow 1961), though broader definitions have since been applied (Pang et al. 2016). Many common terrestrial/freshwater fungal species within Cladosporium Link, Saccharomyces Meyen ex Hansen, Fusarium Link, Aspergillus P. Micheli ex Haller, Penicillium Link can grow in environments with salinity >30 (e.g. Morrison-Gardiner 2002, Schulz et al. 2008). As a result, the definition of a marine fungus is not founded in a unifying evolutionary history, if one exists. Moreover, the genetically encoded underpinnings that interface and give rise to either osmoregulation or osmoconformation within any broadly distributed marine-terrestrial fungal species remain to be fully elucidated.
The known long-distance travel of fungal spores through the atmosphere (Hovmøller et al. 2008) and inferred sourcing of fungal spores from local terrestrial environments like pollen (Heusser 1988), indicate that a fraction of fungi detected in the marine realm are almost certainly of terrestrial origin (Frölich-Nowoisky et al. 2012). Some research postulates that aquatic environments may be an ideal place for long distance fungal dispersal to occur (Golan and Pringle 2017). Furthermore, some fungal species that are known to exist both on land and in the marine realm can survive in seawater for at least 8 months (Hassett et al. 2019). Paired with 0.13 m s−1 current velocity (Johnson and McPhaden 2001), some fungi can theoretically travel the distance between New Zealand and Antarctica. Consequently, the abundance and corresponding genetically detected biogeography of marine fungi should certainly be influenced by the reproductive success and subsequent dispersal of terrestrial fungi, especially among many members of the Dikarya, which are evolved for aerial dispersal (James et al. 2006). Despite substantial overlapping range distributions, freshwater and marine fungal communities are significantly different (Panzer et al. 2015).
Efforts to assess the composition of marine fungal communities have been guided by contemporary taxonomy (i.e. defining what was considered to be within the Fungi), and constrained by sampling effort and the application of the most-advanced methodologies to inform ecology (e.g. culturing versus cloning versus high-throughput sequencing). For example, given their ease of cultivation, marine yeasts within the Ascomycota and Basidiomycota were historically believed to be the most abundant fungi in the pelagic ocean (Fell 2012), despite their variable density (1–200 cells l−1) in seawater (Nagahama 2006, Fell 2012). Sequencing efforts have identified marine fungal taxa with known yeast forms as some of the most abundant fungi (Bass et al. 2007, Panzer et al. 2015). However, with increasing capacity to sample an environmental community with high-throughput sequencing (HTS), novel insights (e.g. hyperabundances of zoosporic taxa) are being generated. From these combined efforts, every fungal phylum has now been detected in the ocean.
The use of HTS has resulted in an increased focus on the zoosporic fungi and their relevance in aquatic microbial food webs (Grossart et al. 2016). Intuitively, flagellated fungi seem most suited for life in the open ocean, as they possess phototactic (Kazama 1972) and chemotactic (Muehlstein et al. 1988) motility, which can be used to overcome sinking and low substrate concentrations in pelagic marine environments; however, other fungi are able to modulate their own sinking rates by possessing spherical lipid complexes that can confer buoyancy (Grolig et al. 2006). In the euphotic zone, the zoosporic Chytridiomycota fungi occupy a niche by seasonally parasitizing diatoms, particularly during blooms (Hassett and Gradinger 2016, Taylor and Cunliffe 2016). Diatoms’ silica frustules serve as an effective barrier to many types of grazers (Hamm et al. 2003). Yet, Chytridiomycota can break through this barrier and, upon maturation, produce numerous zoospores that can be consumed by zooplankton. This trophic dynamic, termed the “mycoloop” (Kagami et al. 2014), likely contributes to organic matter recycling and biological turnover within the euphotic zone, which confers a potential reduction of particulate organic matter export. The relevance of the Chytridiomycota to higher trophic levels appears to be seasonal, generally peaking during the spring phytoplankton bloom in high-latitude marine environments (Cleary et al. 2017). Other zoosporic fungi include the Aphelida, Cryptomycota, Neocallimastigomycota, Olpidiomycota, and select members formerly within the Zygomycota, whose relative abundance in marine environments appears to be generally low, according to the limited number of DNA studies (e.g. Cheung et al. 2010, Jebaraj et al. 2010, Livermore and Mattes 2013, Hassett and Gradinger 2016), though with emerging exceptions (Rojas-Jimenez et al. 2019). While we can infer the ecological roles of these understudied fungi in the pelagic ocean from their better-characterized freshwater counterparts, there is currently little evidence outside of sporadic observations that they exist in marine environments.
The objective of this review is to summarize the known diversity, richness, and associated spatial patterns of the marine fungi. Specifically, we focus this review on planktonic fungi, including those fungi from the coastal (defined here as marine systems disproportionally influenced by the terrestrial realm, including the Mediterranean Sea and Baltic Sea) and pelagic realms. We first summarize the morphology based diversity and then supplement this compilation by analyzing publicly available nucleotide sequencing databases. The efforts of various HTS studies from across the world provide a unique opportunity to explore global patterns of planktonic fungal diversity. To capitalize on the availability of these datasets, we reprocessed and analyzed 659 (286 18S rRNA amplicon and 373 shotgun sequencing) datasets from across the world (Figure 1).
Morphologic-based diversity of planktonic marine fungi
Historically, studies of marine fungal diversity relied on microscopy and subsequent morphological identification (e.g. Sparrow 1973) to assess community composition. Many historical descriptions of marine fungi detailed diversity to a higher taxonomic level (e.g. Horner and Schrader 1982), or not at all. For example, Höhnk (1961) isolated fungi from seawater during a voyage of the Anton Dohrn for the International Geophysical Year project on the Greenland Shelf; however, even the taxonomic phylum from many of these fungi was never determined. Partially in response to the seemingly low concentration of planktonic marine fungi, substrates were used to increase the success of recovery to expand descriptions of diversity. Specifically, sterile manmade panels (Jones and Le Campion-Alsumard 1970) and wood were deployed into the sea (Meyers and Reynolds 1958, Byrne and Jones 1974) at depths up to 3975 m (Kohlmeyer and Kohlmeyer 1979). From these early efforts, approximately 60 pelagic fungi were recovered from test timber blocks (Table 1). Nearly all recovered species were identified as common, globally distributed yeasts and Ascomycota, such as Antennospora quadricornuta (Cribb et J.W. Cribb) T.W. Johnson, Cirrenalia macrocephala (Kohlm.) Meyers et Moore, and Trichocladium alopallonella (Meyers et Moore) Kohlm. et E. Kohlm. A few fungi were recovered from only one location, hinting at discrete geographic localization. As some of these recovered fungi are very common in sea foam (Tokura et al. 1982), Nakagiri 1989), the perceived discrete patterns detected in these early studies are now known to be driven by sampling effort (Finlay 2002).
Selected common marine fungi recovered by submerging wood test panels.
|Alternaria maritima G.K. Sutherl.||UK, Sweden, South Africa (Atlantic Coast)|
|Antennospora quadricornuta (Cribb et J.W. Cribb) T.W. Johnson||Antigua, Bahama, Haiti, British Honduras, Kuwait, Puerto Tahiti, Trinidad|
|Ceriosporopsis halima Linder||Antarctic (Atlantic coast), Argentina, Denmark, Japan, Sweden, South Africa|
|Cirrenalia macrocephala (Kohlm.) Meyers et R.T. Moore||France, Denmark, Ghana, Hong Kong, India, Italy, Japan, Sweden, UK, USA|
|Corollospora maritima Werderm.||Alaska, Antarctic, Argentina, Canada, Italy, Japan, South Africa|
|Dictyosporium pelagica (Linder) G.C. Hughes ex E.B.G. Jones||France, Denmark, Ghana, Hong Kong, Kuwait, UK|
|Halenospora varia (Anastasious) E.B.G. Jones||Hong Kong, Italy, Sweden, USA (Salton Sea)|
|Halosphaeria appendiculata Linder||Canada, Denmark, Italy, Kuwait, South Africa (Atlantic coast) Sweden, UK|
|Halosphaeriopsis mediosetigera (Cribb et J.W. Cribb) T.W. Johnson||Alaska, Antarctic, Argentina, Canada, Italy, Japan, South Africa|
|Lulwoana uniseptata (Nakagiri) Kohlm., Volkm.-Kohlm., J. Campb., Spatafora et Gräffenhan (Often as asexual morph Zalerion maritimum)||Antarctica, Africa (Atlantic Coast), Italy, Kuwait|
|Lulworthia floridana Meyers||Aden, Denmark, India, Italy, Singapore, Africa (Atlantic Coast), UK|
|Nereiospora comata (Kohlm.) E.B.G. Jones, R.G. Johnson et S.T. Moss||Antarctica, Denmark, Ghana, Italy, Sweden, UK|
|Nereiospora cristata (Kohlm.) E.B.G. Jones, R.G. Johnson et S.T. Moss|
(Often as asexual morph Monodictys pelagica)
|Denmark, France, Hong Kong, Italy, Sweden|
|Okeanomyces cucullatus (Kohlm.) K.L. Pang et E.B.G. Jones|
(Often as asexual morph Periconia prolifica)
|Indonesia, Ghana, Hong Kong, Kuwait|
|Remispora quadri-remis (Höhnk) Kohlm.||France, Ghana, Hong Kong, Italy, Sweden|
|Trichcladium alopallonella (Meyers et R.T. Moore) Kohlm. et Volkm.-.Kohlm.||Aden, Alaska, Antarctic, Argentina, Canada, Ghana, Hong Kong, India, Sweden, UK|
Yeasts were some of the earliest observed and remain one of the most well-documented groups within the marine fungi (Kriss et al. 1952, Meyers et al. 1967a) that are now known to have a global distribution (Kriss 1963, Fell 1976, Kohlmeyer and Kohlmeyer 1979). Some yeasts appear to have a limited dispersal, such as select Metschnikowia Kamienski species in tropical waters in the Indian Ocean, Blastomyces parvus (Emmons et Ashburn) Jiang, Sigler et de Hoog in warmer Antarctic waters (Fell and Statzell-Tallman 1971), and Candida natalensis (van der Walt et Tscheuschner) south of the Indo-Pacific polar front. Yeasts are regularly found in unexpected environments, such as the sulfidic depth of the Black Sea (Kriss 1963) and up to ~4000 m depth (e.g. Nagahama 2006), underscoring their wide distribution.
Yeasts are a polyphyletic group of organisms belonging to the Ascomycota and Basidiomycota that are usually characterized by unicellular growth (Kutty and Philip 2008), Fell 2012). The study of marine pelagic yeasts was stimulated by the US Program in Biology, International Indian Ocean Expedition through which 25 species of yeasts were eventually isolated (Fell 1967). Currently, 214 marine yeast species in 65 genera (27 families) are known to exist in marine environments (Jones et al. 2019). Common genera of marine yeasts include Cryptococcus Vuill., Debaryomyces Lodder et Kreger-van Rij ex Kreger-van Rij, Metschnikowia, Candida Berkhout, Torulopsis Cif., Rhodotorula F.C. Harrison, Kluyveromyces Van der Walt, and Rhodosporidium Banno (Kutty and Philip 2008). However, most marine yeasts belong to the genera Candida (64 species), Rhodotorula (10 species), Pichia E.C. Hansen, and Kazachstania Zubkova (Table 2). Species within Candida and Rhodotorula appear to be the predominant genera encountered in culturing-based studies (Fell 2012, Jones et al. 2015). Ecologically, marine yeasts are known to degrade a wide range of biomass and hydrocarbons, and parasitize marine macrofauna (Kutty and Philip 2008). While observations indicate that yeast abundance is correlated with substrate availability (Nagahama 2006), there is still a lack of understanding of the environmental controls that regulate their distribution and activity.
Pelagic marine yeasts isolated from seawater.
|Azymocandida mycoderma (Reess) E.K. Novák et Zsolt (=Candida mycoderma (Reess) Lodder et Kreger-van Rij)||Indian Ocean||Fell (1967)|
|Blastobotrys parvus (Fell et Statzell) Kurtzman et Robnett||Southern Ocean||Fell (1976, 2012)|
|Candida acidothermophilum Masuda, Kato, Takayama, Kida et Nakan.||North Sea||Meyers et al. (1967b)|
|C. albicans (C.P. Robin) Berkhout||Indian Ocean||Fell (1967)|
|C. atlantica (Siepmann) S.A. Mey. et Simione||Atlantic Ocean; Indian Ocean||Fell (1967), Meyers (1967b)|
|C. atmosphaerica Santa María||Indian Ocean||Fell (1967)|
|C. diddensii (Phaff, Mrak et O.B. Williams) Fell et S.A. Mey.||North Sea, Black Sea||Meyers et al. (1967a),b)|
|C. glabrata (H.W. Anderson) S.A. Mey. et Yarrow||Taiwan||Chen et al. (2009)|
|C. guilliermondii (Castell.) Langeron et Guerra||Black Sea, Indian Ocean||Meyers (1967a), Fell (1967)|
|C. lusitaniae Uden et Carmo Souza||North Sea||Meyers et al. (1967a)|
|C. natalensis Van der Walt et Tscheuschner||Pacific Ocean||Fell (1976)|
|C. neustonensis C.F. Chang et S.M. Liu||Taiwan||Chang et al. (2010)|
|C. parapsilosis (Ashford) Langeron et Talice||Biscayne Bay, Black Sea, Indian Ocean||Roth et al. (1962), Fell (1967), Meyers et al. (1967a)|
|C. polymorpha Y. Ohara et Nonom. ex M.T. Sm. et Batenburg-van der Vegte||Black Sea, Indian Ocean||Fell (1967), Meyers (1967a)|
|C. rugosa (H.W. Anderson) Diddens et Lodder||Indian Ocean||Fell (1967)|
|C. suecica Rodr. Mir. et Norkrans||Swedish west coast||De Mianda and Norkrans (1968)|
|C. tenuis Diddens et Lodder||Indian Ocean||Fell (1967)|
|C. torresii (Uden et Zobell) S.A. Mey. et Yarrow||Torres Strait, Australia||Van Uden and Zobell (1962)|
|C. tropicalis (Castell.) Berkhout||North Sea, Black Sea, Bombay, Taiwan, Indian Ocean||Fell (1967), Meyers et al. (1967a),b), Chen et al. (2009)|
|C. zeylanoides (Castell.) Langeron et Guerra||North Sea||Meyers et al. (1967b)|
|Cyberlindnera jadinii (Sartory, R. Sartory, Weill et J. Mey.) Minter||Black Sea||Meyers et al. (1967a)|
|Cryptococcus infirmominiatus (Okun.) Phaff et Fell||Black Sea||Meyers et al. (1967a)|
|Cystobasidium pallidum (Lodder) A.M. Yurkov, Kachalkin, H.M. Daniel, M. Groenew., Libkind, V. de Garcia, Zalar, Gouliamova, Boekhout et Begerow (= old name Rhodotorula palida Lodder)||Indian Ocean||Fell (1967)|
|Cy. minutum (Saito) A.M. Yurkov, Kachalkin, H.M. Daniel, M. Groenew., Libkind, V. de Garcia, Zalar, Gouliamova, Boekhout et Begerow||North Sea, in a bloom of Noctiluca milliare, Black Sea||Fell (1967), Meyers et al. (1967a),b)|
|Debaryomyces hansenii (Zopf) Lodder et Kreger||Atlantic Ocean, North Sea, Bombay, Indian Ocean||Bhat and Kachwalla (1955), Meyers et al. (1967b)|
|Glaciozyma antarctica (Fell, Statzell, I.L. Hunter et Phaff) Turchetti, Connell, Thomas-Hall et Boekhout||Atlantic Ocean||Fell et al. (1969)|
|Hanseniaspora uvarum (Niehaus) Shehata, Mrak et Phaff ex M.T. Sm.||North Sea, Taiwan, Indian Ocean||Fell (1967), Meyers et al. (1967b), Chen et al. (2009)|
|Kazachstania jiainica C.F. Lee et Chun H. Liu||Taiwan||Chen et al. (2009)|
|K. yakushimaensis (Mikata et Ueda-Nishim.) Kurtzman||East Taiwan||Chen et al. (2009)|
|Kluyveromyces aestuarii (Fell) Van der Walt||Torres Strait, Australia||Van Uden and Zobell (1962)|
|Kodamaea ohmeri (Etchells et T.A. Bell) Y. Yamada, Tom. Suzuki, M. Matsuda et Mikata||East Taiwan||Chen et al. (2009)|
|Leucosporidium scottii Fell, Statzell, I.L. Hunter et Phaff||Atlantic Ocean, Atlantic Peninsula, Weddell Sea||Fell et al. (1969)|
|Metschnikowiella bicuspidata (Metschn.) T. Kamienski||Pacific Ocean||Fell (1976)|
|M. krissii (Uden et Cast.-Branco) Uden||Pacific Ocean||Fell (1976)|
|M. zobellii (Uden et Cast.-Branco) Uden||Atlantic Ocean||Meyers et al. (1967b)|
|Meyerozyma guilliermondii (Wick.) Kurtzman et M. Suzuki||Bombay||Fell (1967)|
|Moesziomyces bullatus (J. Schröt.) Vánky||Atlantic||Meyers et al. (1967b)|
|Naganishia albida (Saito) Xin Zhan Liu, F.Y. Bai, M. Groenew. et Boekhout [old name=Cryptococcus albidus (Saito) C.E. Skinner]||Black Sea, Indian Ocean||Fell (1976), Meyers et al. (1967a)|
|Papiliotrema laurentii (Kuff.) Xin Zhan Liu, F.Y. Bai, M. Groenew. et Boekhout||Black Sea||Meyers et al. (1967a)|
|Pichia fermentans Lodder||Indian Ocean||Fell (1967)|
|P. kudriavzevii, Boidin, Pignal et Besson||East Taiwan||Chen et al. (2009)|
|Prosporobolomyces hispanicus (=Sp. hispanicus) Peláez et C. Ramírez) E.K. Novák et Zsolt||Indian Ocean||Fell (1967)|
|Rhodotorula babjevae (Golubev) Q.M. Wang, F.Y. Bai, M. Groenew. et Boekhout||Atlantic||Meyers et al. (1967b)|
|R. diobovata (S.Y. Newell et I.L. Hunter) Q.M. Wang, F.Y. Bai, M. Groenew. et Boekhout||Atlantic Ocean||Meyers et al. (1967b)|
|R. dairenensis (T. Haseg. et Banno) Fell, Samp. et Gadanh||Biscayne Bay||Roth et al. (1962)|
|R. glutinis (Fresen.) F.C. Harrison||Black Sea, Indian Ocean||Meyers et al. (1967a), Fell (1976)|
|R. graminis Di Menna||Black Sea, Indian Ocean||Fell (1967), Meyers et al. (1967a)|
|R. mucilaginosa (A. Jörg.) F.C. Harrison (= Rhodotorula rubra (Demme) Lodder)||Biscayne Bay, North Sea, Black Sea, Indian Ocean||Roth et al. (1962), Fell (1967), Meyers et al. (1967a),b)|
|Rhodosporidiobolus odoratus (= Sp. odorus) (J.P. Samp., Fonseca et E. Valério) Q.M. Wang, F.Y. Bai, M. Groenew. et Boekhout||Indian Ocean||Fell (1967)|
|Saccharomyces cerevisiae Meyen ex E.C. Hansen||Indian Ocean||Fell (1976)|
|Sa. chevalieri Guillierm.||Bombay||Bhat and Kachwalla (1955)|
|Sa. italicus T. Castelli||Bombay||Bhat and Kachwalla (1955)|
|Spencerozyma crocea (= R. crocea) (Shifrine et Phaff) Q.M. Wang, F.Y. Bai, M. Groenew. et Boekhout||Indian Ocean||Fell (1967)|
|Sporobolomyces pararoseus H.C. Olson et B.W. Hammer||Black Sea, North Sea||Meyers et al. (1967a),b)|
|Sp. roseus Kluyver et C.B. Niel||Black Sea||Meyers et al. (1967a)|
|Sterigmatomyces halophilous (= Sp. halophilous) Fell||Indian Ocean||Fell (1967)|
|Torulaspora delbrueckii (Lindner) Lindner||Bombay, East Taiwan||Bhat and Kachwalla (1955), Chen et al. (2009)|
|Wickerhamomyces anomalus (E.C. Hansen) Kurtzman||Taiwan||Chen et al. (2009)|
As culturing poses a serious bottleneck at recovering diversity, and global sampling efforts are too few to determine large patterns of diversity, we chose to explore diversity and richness through nucleotide-based studies by mining publicly available sequencing databases and conducting analyses on phylogenetically classified nucleotide data.
Marine fungi and high-throughput sequencing
The use of molecular phylogenetics to understand fungal evolution and inform taxonomy has generated extensive databases of nucleotide data derived from cultured and uncultured fungi. These databases are, in turn, used to inform HTS studies of richness and diversity. An extensive search of the National Center for Biotechnology Information (NCBI) nucleotide database (Supplementary methods) revealed that only half of the fungi known to exist in the marine realm are represented by a DNA locus (Supplementary Figure S1) of either terrestrial or marine origin. Of the marine fungi that are represented by a DNA sequence, the majority are represented by the large ribosomal subunit (28S rRNA), followed by the internal transcribed spacer (ITS) region, and finally the small ribosomal subunit (18S rRNA). The ITS region has been proposed as the formal molecular locus/barcode of fungi (Schoch et al. 2012); however, it is too variable to address the phylogeny of higher taxonomic ranks (Lindahl et al. 2013) without an 18S rRNA complement (Panzer et al. 2015) and disproportionally skews HTS-amplicon-based studies of fungal abundances, relative to loci within the 18S or 28S ribosomal subunit-encoded region (De Filippis et al. 2017). The 28S rRNA subunit is more variable than the 18S rRNA subunit and is consequently more informative for taxonomic resolution of the fungi. However, NCBI’s Sequence Read Archive (SRA) is disproportionally represented by 18S rRNA marine datasets (Panzer et al. 2015), thereby currently necessitating the use of 18S amplicon datasets to surmise any large-scale spatial phenomena.
An alternative approach to single locus amplicon-based studies is shotgun metagenomics. Shotgun sequencing provides a less-biased sequencing approach that is not reliant on primer matches in PCR-based marker gene analyses (Tedersoo et al. 2015). Furthermore, shotgun sequencing can link taxonomy to function through analysis of encoded functional genes (e.g. Morales et al. 2019). However, the successful annotation of extra-rDNA operon data is dependent on curated databases that contain genome-wide information. There are currently (21 November 2018) 3905 fungal genomes archived in NCBI: 3032 from the Ascomycota, 691 from the Basidiomycota, and 182 from other fungal lineages. Combined with annotated transcripts and proteins, these data contribute to NCBI’s RefSeq database that contains molecular data for 85,308 organisms, including 604 fungal genera. Of all 604 represented fungal genera, only 73 marine fungal genera are in the RefSeq database. As only half of the known marine fungal species have been assigned a molecular barcode and only 12% of marine fungal genera are represented in the RefSeq database, the phylogenetic classification and subsequent interpretation of HTS studies seem as limited by molecular information derived from described organisms, as sequences derived from organisms not yet known to science (e.g. Richards et al. 2012). Even though half of the marine fungi do not have any associated molecular data, HTS still offers immense possibilities to understanding global patterns of marine fungal diversity (Nilsson et al. 2018), especially at various taxonomic resolutions (such as phylum level), where databases are likely not as limiting. Even still, hierarchal database taxonomies used for classification can lag substantially behind novel evolutionary insights (Bass et al. 2018) and taxonomic revisions (Tedersoo et al. 2018).
Shotgun sequencing of marine fungal communities
We conducted a global analysis of shotgun sequencing datasets deposited in Metagenomic Rapid Annotations using Subsystems Technology (MG-RAST) (Glass et al. 2010), derived primarily from Global Ocean Sampling Expedition (e.g. Rusch et al. 2007), Tara Oceans (Pesant et al. 2015), Ocean Sampling Day (Kopf et al. 2015), and the Deepwater Horizon oil spill (Yergeau et al. 2015). We selected all marine samples and subsequently filtered out databases that were missing associated metadata, such as GPS location and depth of sampling (Supplementary Methods). After eliminating datasets with missing metadata, we analyzed 373 databases that contained 4.7×109 total sequences (two orders of magnitude more than other studies), of which 1.8×109 were annotated (minimum 65% identity and e-value cutoff=10−8). Analysis of these reads revealed that 4,130,526 (0.22%) of all reads (including non-annotatable reads, prokaryotes, and metazoans) were assigned to fungi. From all datasets, fungi comprised 7.8% of all eukaryotic sequences. Of these reads, the Ascomycota comprised 76.2% of all annotated fungal reads, followed by the Basidiomycota with 18.1%, the Microsporidia at 2.1%, the Chytridiomycota at 1.6%, unclassified fungal reads at 1.3%, the Blastocladiomycota at 0.2%, and finally the Glomeromycota at 0.2%. These proportions are consistent with other shotgun sequencing studies (Morales et al. 2019). When databases were normalized for comparison, these fungal phyla comprised comparable fractions of relative abundances throughout the world’s oceans, irrespective of location, date of sampling, or environmental conditions (Supplementary Figure S2).
Site-specific spatial analysis using non-metric multidimensional scaling (NMDS) revealed a single mixed cluster of samples whose spatial distance was influenced by a predominance of Ascomycota, Basidiomycota, and the Microsporidia (Supplementary Figure S2). The mechanism for the grouping of the Microsporidia with the Dikarya is uncertain; however, the closely related Cryptomycota are known parasites of other fungi (Letcher et al. 2017). Spatially segregating, non-grouping sites were dominated by the Blastocladiomycota, Chytridiomycota, unclassified fungi, and the Glomeromycota. As many reads were not classified, it is difficult to conclusively discern biological patterns in light of known database limitations that result in a high proportion of sequences without annotations. Still, the tight clustering of many sample sites predominated by the Dikarya and Microsporidia suggest that a core group of these fungi could predominate in planktonic marine fungal communities and that site-specific characteristics could disproportionally favor the growth of Chytridiomycota, Blastocladiomycota, and Glomeromycota. The co-occurrence of an oil spill in the Gulf of Mexico and the hyperabundance of several fungal phyla after this spill support this hypothesis, especially as some phylogenetically basal fungi are known degraders of recalcitrant substances (Powell 1993). Alternatively, environmental filtering can eliminate taxa with less tolerance to perturbations or stressful environmental conditions, suggesting that basal fungi might be more tolerant to environmental irregularities. Regardless, the generally homogenous proportions of phyla detected across all sites was surprising.
The homogeneous patterns observed by analyzing sequences at the taxonomic phylum level led us to suspect that we were masking discernible abundance differences. However, even at the genus level, we identified a similar homogeneous pattern of fungal taxa throughout the world’s oceans (Figure 2). The stable community composition of classified fungal genera across different marine ecosystems remains surprising, especially as the less biased approach of shotgun sequencing (i.e. no amplification) lends greater confidence to their real proportions in the environment. Future research can leverage metatranscriptomic analyses of RNA to help discern the active versus the latent fungal fraction in the environment.
Site-specific spatial analysis of genera-classified reads revealed, again, one large cluster of spatially grouping samples (Figure 2). This main cluster was supplemented by spatially outlying sites predominated by sequences classified as Allomyces E.J. Butler (Blastocladiomycota), Smittium R.A. Poisson (Kickxellomycota), and Mortierella Coem. (Mucoromycota). Statistical analysis (two-way ANOVA) using Inverted Simpson diversity estimates identified that the Gulf of Mexico was statistically different (p<0.00002) from all sites. Supplemental analysis using Chao1 identified that the Gulf of Mexico was statistically different from the North Pacific (two-way ANOVA, p=0.00005), South Pacific (p=0.0014), and Indian Ocean (p=0.009). These differences were likely driven by the oil spill, as well as the metatranscriptomics data that comprised the majority of these data within this sampling site. Excluding the Gulf of Mexico, there were no differences in fungal diversity between any of our samples, irrespective of oceanographic regions, water depth, or proximity to land (Supplementary Figure S3). Rarefaction analysis of fungal genera suggests that most pelagic marine sites have approximately 40 fungal genera (Figure 2). However, few rarefaction curves actually reached a true asymptote, suggesting that a large fraction of fungal diversity either exists in lower quantities and can only be recovered with significant shotgun sequencing of the environment or that recovered diversity is not detectable due to current database limitations.
Marine fungal community analysis using 18S rRNA genes
The potential cost constraints associated with achieving adequate sequencing depth to adequately sample a community, as well as current database limitations associated with shotgun sequencing, pose major constraints to describing marine fungal diversity. Amplicon-based HTS analyses, in principle, offer a targeted approach to inventorying taxonomically informative loci, especially for those taxa that might exist in lower abundances. Analysis of 286 NCBI 18S rRNA databases (Table S1, Supplementary methods) that represent HTS studies conducted in various seas and oceans (e.g. Celussi et al. 2018; Enberg et al. 2018; Edgcomb et al. 2011; Flaviani et al. 2018; Hassett et al. 2017; Pearman et al. 2017; Stern et al. 2015) identified that the fungi comprised 1.3% of all eukaryotic sequences from marine environmental datasets. Many fungal sequences from this study were only classifiable to higher taxonomic levels (Figure 3), consistent with other published findings (Comeau et al. 2016, Hassett et al. 2017, Nagano et al. 2017, Picard 2017), suggesting either novel lineages and/or under-populated reference databases. Of the SILVA-classified fungi, the Ascomycota comprised on average 43% of all fungi globally, followed by the Chytridiomycota with 36%, the Basidiomycota with 27%, and then other fungal clades that contributed less than 1% of relative abundance. The under-representation of the Microsporidia and elevated Chytridiomycota in amplicon-based studies, relative to our shotgun sequencing data, is apparent and could indicate primer bias associated with the amplification of these groups.
Site-specific NMDS of individual samples revealed spatially partitioned fungal communities within different oceans. Indicator taxa within the Dikarya comprised a central-grouping core, while sites with flagellated fungal indicator species spatially segregated to the margins (Supplementary Figure S4). The homogeneous pattern of fungal taxa observed through shotgun sequencing was not observed with amplicon sequencing, consistent with other studies that found varying environmental conditions structuring fungal communities (Jeffries et al. 2016).
Globally, over the entire ocean depth, the mean salinity over the last 10 years is 34.6 with a standard deviation of 1 (Supplementary methods). The Baltic Sea and the Red Sea are both connected to the global ocean through one very narrow and shallow opening; hence, their salinity is mostly controlled by precipitation/evaporation and river runoff. As many large snow-melt fed rivers flow into the Baltic (Bergström and Carlsson 1994), the top 100 m of that sea are extremely fresh (average salinity of 7, or 27 standard deviations away from the global average). The Red Sea in contrast is characterized by year-round evaporation (Sofianos et al. 2002), making that sea hypersaline (average salinity of 40, or 5 standard deviations away from the global average). Marine environments with atypical salinity regimes, such as sea ice and the Red Sea, as well as the estuarine Baltic Sea, had elevated proportions of Chytridiomycota (Supplementary Figure S4), irrespective of substantially different temperatures, which structure marine microbial (Sunagawa et al. 2015) and terrestrial fungal communities (Kivlin et al. 2011). These results support the known effects of salinity on structuring marine fungal communities (Mohamed and Martiny 2011), especially in the Baltic Sea (Rojas-Jimenez et al. 2019). The Red Sea, Baltic Sea, and Arctic sea ice sites were all sequenced with the Illumina platform. This consistency helps ameliorate any concerns associated with conclusions derived from comparisons across sequencing platforms. The abundances and relevance of the Chytridiomycota at global scales remain largely unknown, as their recoverability and associated diversity in culturing surveys appears low (Jones et al. 2015), relative to the recoverability of their DNA and associated clone-based diversity from the marine environment (Hassett et al. 2017).
Spatial partitioning of fungal groups was also evident at the genus level, where about 40 groups constituted the majority of fungal observation (Figure 3). The highest richness was detected in samples from >100 m depth in the Mediterranean Sea and shallow samples within the Baltic Sea. The lowest diversity was detected in the Red Sea (Supplementary Figure S5). In the Arctic Ocean and Baltic Sea, Chytridiomycota members with closest affinity to the Lobulomycetales contributed large fractions of total fungal observations, as described previously (Hassett et al. 2017). In the Bering Sea and Red Sea, Chytridiomycota communities were comprised of sequences with closest affinity to the Gromochytriales and Rhizophydiales. The Rhizophydiales is the largest and most diverse of all Chytridiomycota orders that contains described marine isolates (Lepelletier et al. 2014). Furthermore, the Gromochytriales and Lobulomycetales are under-populated Chytridiomycota taxonomic orders that contain seven (Seto and Degawa 2015, Van den Wyngaert et al. 2018) and two species, respectively (Karpov et al. 2018); consequently, it is not surprising to detect Chytridiomycota sequences with closest affinity to these under-populated orders. Unclassifiable members within the fastidious and enigmatic Malassezia Baill. (Amend 2014), as well as common fungi within the Leotiomycetes, Trichocomaceae, Hypocreales, Cadophora Lagerb. et Melin, and numerous yeast-forming species were frequently detected. Marine yeasts primarily within the genera Rhodotorula, Naganishia Goto, Saccharomyces, and Zygosaccharomyces Nishiw. were found frequently in hypersaline environments, and were supplemented by contributions from Pichia, Wickerhamomyces Kurtzman, Robnett et Basehoar-Powers, and Hortaea Nishim. et Miyaji. Pigmented yeasts within Rhodotorula were detected in Arctic Ocean sea ice near Svalbard, along with Naganishia, which contains species isolated from hypersaline environments (Fotedar et al. 2018). Zygosaccharomyces was detected almost exclusively from the Red Sea.
Site-specific spatial analysis of these samples revealed that spatially segregated sites were driven by a predominance of fungi classified within the Chytridiomycota taxonomic order Rhizophydiales, as well as taxa allied to Pichia, Scleroderma Pers., Wickerhamomyces, Hyphodontia J. Erikss., and Fusarium. Rarefaction analysis of fungal genera suggests that most pelagic marine sites have approximately 20–30 classifiable fungal genera, plus a substantial fraction of unclassified sequences binned at the phylum level that would likely inflate the number of detected genera considerably. We used a combination of rarefaction curves, diversity indices, and Analysis of Similarities (ANOSIM; Table 3) to determine whether fungal communities in different oceanographic regions were distinct. Some sites were significantly different, such as the Arctic Ocean and Red Sea, while other sites were quite similar, such as the ice-covered Arctic Ocean and Baltic Sea. The most similar regions were the Bering Sea and the Cariaco Basin. Several sites were equally dissimilar (e.g. Arctic Ocean-Ligurian Sea, Baltic Sea-Red Sea, South Indian Ocean-Ligurian Sea). These large regional differences in fungal community structure are consistent with smaller regional studies that found community composition changing with distance from shore (Burgaud et al. 2013) and different nutrient regimes (Jeffries et al. 2016). As environmental conditions selectively favor the growth of specific taxa at smaller scales, it is not surprising to find statistically different fungal communities associated with larger distinct oceanographic regions. Though these phylogenetically classified sequences are informative, more distance-based analysis, such as those being generated through UniEuk (Berney et al. 2017) will help to more accurately elucidate biogeography.
Two-way ANOSIM analysis displaying false discovery rate-adjusted p-values.
|Arctic Ocean||Baltic Sea||Bering Sea||Cariaco Basin||English Channel||Florida Keys||Liguarian Sea||Red Sea|
|South Indian Ocean||0.018||0.004||0.12||0.2647||0.0252||0.12||0.0055||0.0208|
Latitudinal gradients of marine fungi and biological hotspots
In terrestrial environments, the decline of species richness with increasing latitude has remained a central dogma in global biogeography (Hillebrand 2004). Discerning causalities for this covariation remain debatable, but has historically modulated around the nexus of various phenomena to explain endemism, such as: center of origin (Vavilov 1951), geological separation (McCoy and Heck 1976), solar radiation’s effects on evolutionary speed (Rohde 1992), and dispersal (Thorson’s Rule, Mileikovsky 1971), which is further scaled according to latitudinal distribution gradients (Rapopart’s Rule, Stevens 1989). Local diversity is partially structured by large-scale biogeographical patterns of a specific taxon (Wiens and Donoghue 2004) that can be further shaped by disturbances (Townsend et al. 2003), biological interactions (Menge and Sutherland 1976), and seasonality (Marquardt et al. 2016). However, this trend does not necessarily apply to marine environments, especially for microbial life (Sunagawa et al. 2015).
Though larger (>1 mm) organisms display distinct biogeography (De Bie et al. 2012), many eukaryotic microbial taxa do not exhibit discernible spatial partitioning (Finlay 2002). Local:global species ratios for some eukaryotic microbial taxa are quite high (80%, Finlay and Clarke 1999), suggesting that high dispersal rates and the formation of resting spores can produce globally distributed homogeneous resting spores, such as seed banks (Lennon and Jones 2011). Consequently, it was hypothesized that among the eukaryotic microbes, many perceived biogeographical differences are the result of sampling effort (Finlay 2002), as opposed to dispersal limitations. The oceans are a contiguous ecosystem, whose circulation patterns connect all water bodies over decadal to centennial time scales. In marine environments, plankton dispersal is determined by local abundance, which is scaled with body size (Soininem et al. 2013, Villarino et al. 2018). Consequently, communities of smaller organisms are more likely to have a worldwide distribution (Djurhuus et al. 2017, Flaviani et al. 2018, Villarino et al. 2018).
If the success of dispersal is dependent on size and local abundance then the differential and usually complex growth patterns of fungal hyphae (Gutiérrez et al. 2011) should result in uneven dispersal patterns in the marine environment. Our analysis based on shotgun metagenomic reads found no discernible differences between richness or diversity and latitude (Figure 4). Our amplicon-based studies identified the highest richness values at 60° north (Supplementary Figure S6). Overall, we find no evidence for a decrease in diversity or richness with increasing latitude in the marine environment, consistent with other marine studies of fungal diversity (Tisthammer et al. 2016), but different from other shotgun sequencing analyses (Morales et al. 2019).
At global scales, we hypothesize that there are multiple phenomena responsible for the absence of any discernible biogeographical patterns in the datasets we re-analyzed. These reasons can be categorized as limitations driven by the absence of a marine fungal biomarker, the existence of various dispersal phenomena beyond ocean currents, and methodology limitations. First, a combination of differential osmotolerance and uncertain evolutionary histories confounds the conclusive identification of a marine fungus. For example, Zuluaga-Montero et al. (2010) undertook a phylogenetic study of Aspergillus flavus Link strains from terrestrial and marine sites and concluded there is no clade particular to the marine environment. Sivaramanan (2014) grew four common terrestrial fungi (Aspergillus, Cladosporium, Helminthosporium Link and Trichoderma Pers. species) to determine their growth on seawater media and demonstrated their halophilic nature; although of terrestrial origin, they have an individual preference, as well as tolerance, to the marine environment (Saritha et al. 2012). Consequently, if terrestrial input exceeds local marine diversity then a marine biogeography signal would be challenging to discern with amplicon data alone, especially if marine fungal communities are predominated by common terrestrial fungi. Zuccaro et al. (2004) in a phylogenetic study, showed that a new marine Acremonium species isolated from Fucus spp. grouped in a monophyletic marine clade comprising Emericellopsis, Stanjemonium, and Acremonium Link species. Consequently, the discernment of some marine taxa can be achieved with molecular phylogeny. The inconclusive evolutionary history of the marine fungi confounds the application of a working ecological definition. If the ocean really is a massive sink for terrestrial-sourced fungi, of which a large fraction can reproduce in the marine realm, biogeographical patterns of oceanic fungal diversity and richness should be nearly impossible to discern from a marine-exclusive perspective. Regardless, our amplicon-based study found the highest marine fungal genera-based richness in the Baltic Sea and Mediterranean Sea. If the fungi are indeed evolutionarily transitioning from terrestrial environments to the marine ecosystems (Spatafora et al. 1998, Hibbett and Binder 2001), coastal areas and enclosed seas with substantial terrestrial influence should be biological hotspots for marine fungal diversity in various stages of this evolutionary transition. The highest fungal richness that we identified in the Baltic Sea and Mediterranean Sea marginally supports this hypothesis. Furthermore, if multiple independent transitions of fungi from terrestrial to marine environments occurred then there should be several geographic areas with elevated diversity, as a result of more time for speciation.
Second, the small size (~2–10 μm diameter) of conidia is not limiting for dispersal and consequently should be distributed widely (Finlay 2002). In addition, both physical, such as advection (Hovmøller et al. 2008) and biological phenomena, such as the movement of fungal propagules by animals (Singh et al. 2016) aid in the wide-distribution of both marine and terrestrial fungi throughout the marine realm. Moreover, the evolved fungal appendages that aid in flotation and adhesion to substrate (Hyde et al. 1993, Jones 1994), coupled with the known association of fungi with marine driftwood (Rämä et al. 2016), suggests another mechanism for long-distance dispersal in the marine realm that could help eliminate detectable biogeography. Endemic marine fungal taxa should augment the discernment of biogeography and aid in assessments of latitudinal gradients. Marine fungal endemism seems likely, especially with regard to obligate parasites and other biotrophic taxa. Fungi have been found in association with macroalgal communities (Loque et al. 2010) and are known to exist as biotrophs in the terrestrial realm (Tedersoo et al. 2010). Consequently, it seems likely that obligate biotrophs would be associated, and ultimately regionally limited, with endemic marine macroalgae (Phillips 2001). Even still, our data find no evidence of latitudinal gradients, irrespective of likely endemic taxa.
Lastly, taxonomic classification of microbial communities depends on the size, quality, and breadth of reference database, which we tried to circumvent by classifying at various taxonomic levels. Regardless of classification scheme or sequencing approach implemented, we found that databases limited our ability to spatially analyze taxonomically classified sequences, especially those sequences derived from zoosporic fungi. These limitations likely resulted in multiple genera binned at higher taxonomic levels, which may or may not have masked discernible differences between latitude and diversity. Recent surveys using molecular techniques suggest that most planktonic fungal communities are comprised of novel species and assemblages (Wang et al. 2014, Jones et al. 2015, Jeffries et al. 2016). To eventually discern if these observations are driven by truly novel species or, alternatively, described species not yet represented in databases, we have generated a list of marine fungal species that are missing molecular information (Table S2).
By reviewing the biogeography of marine planktonic fungi, we discovered significant gaps in scientific knowledge that challenge our understanding of marine fungal ecology. First, there are few culturing observations from the open ocean, which is primarily a result of under-sampling. Second, we found that ~50% of described marine fungi are missing an assigned rDNA locus in public databases. Third, the substantial range-overlap between terrestrial and marine fungal species underscores the missing evolution-based understanding needed to characterize and define a marine fungus. Though we found no evidence for any relationship between fungal richness/diversity and latitude, as previously described (Morales et al. 2019), it seems possible that if terrestrial fungi are sourced into the sea (Frölich-Nowoisky et al. 2012), and if terrestrial fungal richness decreases with latitude (Tedersoo et al. 2014) then detectable fungal richness in the marine environment should decrease with latitude, especially if the terrestrial input exceeds local marine diversity.
Analysis of fungal community structure identified that the only atypical shotgun sequencing dataset was from the Gulf of Mexico, which was predominated by the Chytridiomycota. These data were supported by amplicon-based sequencing, which found that Chytridiomycota dominated fungal communities in areas with atypical salinity regimes, including the Arctic Ocean, Red Sea, and Baltic Sea. These data suggest that this under-studied clade of marine fungi might have greater ecological relevance than is currently believed. Moreover, the uncharacterized relevance and diversity of other zoosporic taxa, as well as missing molecular information for half of known marine fungi, provides ample opportunities to contribute to marine mycology.
Brandon Hassett and Tobias Vonnahme are funded by UiT – the Arctic University of Norway and the Tromsø Research Foundation under the project “Arctic Seasonal Ice Zone Ecology”, project number 01vm/h15. This work was supported by a grant from the Simons Foundation (award #547606 to Xuefeng Peng). Gareth Jones is supported under the Distinguished Scientist Fellowship Program (DSFP), King Saud University, Kingdom of Saudi Arabia. This work was supported by the VINNOVA Marie Curie Cofund fellowship (award 2015-01487 to Céline Heuzé).
Amend, A. 2014. From dandruff to deep-sea vents: Malassezia-like fungi are ecologically hyper-diverse. PLoS Pathog. 10: e1004277.
Bass, D., A. Howe, N. Brown, H. Barton, M. Demidova, H. Michelle, L. Li, H. Sanders, S.C. Watkinson, S. Willcock and T.A. Richards. 2007. Yeast forms dominate fungal diversity in the deep oceans. Proc. Biol. Sci. 274: 3069–3077.
Bass, D., L. Czech, B.A.P. Williams, C. Berney, M. Dunthorn, F. Mahé, G. Torruella, G.D. Stentiford and T.A. Williams. 2018. Clarifying the relationships between Microsporidia and Cryptomycota. J. Euk. Microbiol. 65: 773–782.
Bergström, S. and B. Carlsson. 1994. River runoff to the Baltic Sea-1950–1990. Ambio 23: 280–287.
Berney, C., A. Ciuprina, S. Bender, J. Brodie, V. Edgcomb, E. Kim, J. Rajan, L.W. Parfrey, S. Adl, S. Audic, D. Bass, D.A. Caron, G. Cochrane, L. Czech, M. Dunthorn, S. Geisen, F.O. Glöckner, F. Mahé, C. Quast, J.Z. Kaye, A.G.B. Simpson, A. Stamatakis, J. Del Campo, P. Yilmaz and C. de Vargas. 2017. UniEuk: time to speak a common language in Protistology! J. Eukaryot. Microbiol. 64: 407–411.
- Export Citation
Berney, C., A. Ciuprina, S. Bender, J. Brodie, V. Edgcomb, E. Kim, J. Rajan, L.W. Parfrey, S. Adl, S. Audic, D. Bass, D.A. Caron, G. Cochrane, L. Czech, M. Dunthorn, S. Geisen, F.O. Glöckner, F. Mahé, C. Quast, J.Z. Kaye, A.G.B. Simpson, A. Stamatakis, J. Del Campo, P. Yilmaz and C. de Vargas. 2017.)| false UniEuk: time to speak a common language in Protistology! J. Eukaryot. Microbiol. 64: 407–411. 10.1111/jeu.12414
Bochdansky, A., M. Clouse and G. Herndl. 2017. Eukaryotic microbes, principally fungi and labyrinthulomycetes, dominate biomass on bathypelagic marine snow. ISME J. 11: 362–373.
Bugni, T. and C.M. Ireland. 2004. Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Nat. Prod. Rep. 21: 143–163.
Burgaud, G., S. Woehlke, V. Rédou, W. Orsi, D. Beaudoin, G. Barbier, J.F. Biddle and V.P. Edgcomb. 2013. Deciphering the presence and activity of fungal communities in marine sediments using a model estuarine system. Aquat. Microb. Ecol. 70: 45–62.
Byrne, P.J. and E.B.G. Jones. 1974. Lignicolous marine fungi. Veroff. Inst. Meerescforsch. Bremerh. Suppl. 5: 301–320.
Celussi, M., G.M. Quero, L. Zoccarato, A. Franzo, C. Corinaldesi, E. Rastelli, M. Lo Martire, P.E. Galand, J.-F. Ghiglione, J. Chiggiato, A. Coluccelli, A. Russo, A. Pallavicini, S. Fonda Umani, P. Del Negro and G.M. Luna. 2018. Planktonic prokaryote and protist communities in a submarine canyon system in the Liguarian Sea (NW Mediterranean). Prog. Oceanogr. 168: 210–221.
- Export Citation
Celussi, M., G.M. Quero, L. Zoccarato, A. Franzo, C. Corinaldesi, E. Rastelli, M. Lo Martire, P.E. Galand, J.-F. Ghiglione, J. Chiggiato, A. Coluccelli, A. Russo, A. Pallavicini, S. Fonda Umani, P. Del Negro and G.M. Luna. 2018. Planktonic prokaryote and protist communities in a submarine canyon system in the Liguarian Sea (NW Mediterranean).)| false Prog. Oceanogr. 168: 210–221. 10.1016/j.pocean.2018.10.002
Chakravarity, D. 1974. On the ecology of the infection of the marine diatom Coscinodiscus granii by Lagenisma coscinnodisci in the Weser Estuary. Veroff. Inst. Meerescforsch. Bremerh. 5: 115–122.
Chang, C., C.F. Lee and S.M. Liu. 2010. Candida neustonensis sp. nov., a novel ascomycetous yeast isolated from the sea surface microlayer in Taiwan. Antonie Van Leeuwenhoek 97: 35–40.
Chen, Y.S., F. Yanagida and L.Y. Chen. 2009. Isolation of marine yeasts from coastal waters of north eastern Taiwan. Aquat. Biol. 8: 55–60.
Cheung, M., C.H. Au, K.H. Chu, H.S. Kwan and C.K. Wong. 2010. Composition and genetic diversity of picoeukaryotes in subtropical coastal waters as revealed by 454 pyrosequencing. ISME J. 4: 1053–1059.
Cleary, A.C., J.E. Søreide, D. Freese, B. Niehoff and T.M. Gabrielsen. 2017. Feeding by Calanus glacialis in a high arctic fjord: potential seasonal importance of alternative prey. ICES J. Mar. Sci. 74: 1937–1946.
Comeau, A.M., F.V. Warwick, L. Bernier and C. Lovejoy. 2016. Novel chytrid lineages dominate fungal sequences in diverse marine and freshwater habitats. Sci. Rep. 6: 30120.
De Bie, T., L. De Meester, L. Brendonck, K. Martens, B. Goddeeris, D. Ercken, H. Hampel, L. Denys, L. Vanhecke, K. Van der Gucht, J. Van Wichelen, W. Vyverman and S.A. Declerck. 2012. Body size and dispersal mode as key traits determining metacommunity structure of aquatic organisms. Ecol. Lett. 15: 740–747.
- Export Citation
De Bie, T., L. De Meester, L. Brendonck, K. Martens, B. Goddeeris, D. Ercken, H. Hampel, L. Denys, L. Vanhecke, K. Van der Gucht, J. Van Wichelen, W. Vyverman and S.A. Declerck. 2012. Body size and dispersal mode as key traits determining metacommunity structure of aquatic organisms.)| false Ecol. Lett. 15: 740–747. 10.1111/j.1461-0248.2012.01794.x
De Filippis, F., M. Laiola, G. Blaiotta and D. Ercolini. 2017. Different amplicon targets for sequencing-based studies of fungal diversity. Appl. Environ. Microbiol. 83: e00905–17.
Debbab, A., A.H. Aly and P. Proksch. 2013. Mangrove derived fungal endophytes – a chemical and biological perception. Fungal Divers. 61: 1–27.
Djurhuus, A., P.H. Boersch-Supan, S.O. Mikalsen and A.D. Rogers. 2017. Microbe biogeography tracks water masses in a dynamic oceanic frontal system. R. Soc. Open Sci. 4: 170033.
Edgcomb, V., W. Orsi, J. Bunge, S. Jeon, R. Christen, C. Leslin, M. Holder, G.T. Taylor, P. Suarez, R. Varela and S. Epstein. 2011. Protistan microbial observatory in the Cariaco Basin, Caribbean. I. Pyrosequencing vs Sanger insights into species richness. ISME J. 5: 1344–1356.
- Export Citation
Edgcomb, V., W. Orsi, J. Bunge, S. Jeon, R. Christen, C. Leslin, M. Holder, G.T. Taylor, P. Suarez, R. Varela and S. Epstein. 2011. Protistan microbial observatory in the Cariaco Basin, Caribbean. I. Pyrosequencing vs Sanger insights into species richness.)| false ISME J. 5: 1344–1356. 10.1038/ismej.2011.6 21390079
Enberg, S., M. Majaneva, R. Autio, J. Blomster and J.-M. Rintala. 2018. Phases of microalgal succession in sea ice and the water column in the Baltic Sea from autumn to spring. Mar. Ecol. Prog. Ser. 599: 19–34.
Fell, J. 1967. Distribution of yeasts in the Indian Ocean. Bull. Mar. S17: 454–470.
Fell, J. 1976. Yeasts in oceanic regions. In: (E.B.G. Jones, ed.) Recent advances in marine mycology. Elek Science, UK. pp. 93–124.
Fell, J. 2012. Yeasts in marine environments. In: (E.B.G. Jones and K.L. Pang, eds.) Marine fungi and fungal-like organisms. Walter de Gruyter, Berlin. pp. 91–102.
Fell, J. and A. Statzall-Tallman. 1971. Sympodiomyces gen. n., a yeast-like organism from southern marine waters. Antonie Van Leeuwenhoek 37: 359–367.
Flaviani, F., D.C. Schroeder, K. Lebret, C. Balestreri, A.C. Highfield, J.L. Schroeder, S.E. Thorpe, K. Moore, K. Pasckiewicz, M.C. Pfaff and E.P. Rybicki. 2018. Distinct oceanic microbiomes from viruses to protists located near the Antarctic Circumpolar Current. Front. Microbiol. 9: 1474.
- Export Citation
Flaviani, F., D.C. Schroeder, K. Lebret, C. Balestreri, A.C. Highfield, J.L. Schroeder, S.E. Thorpe, K. Moore, K. Pasckiewicz, M.C. Pfaff and E.P. Rybicki. 2018. Distinct oceanic microbiomes from viruses to protists located near the Antarctic Circumpolar Current.)| false Front. Microbiol. 9: 1474. 30065704 10.3389/fmicb.2018.01474
Fotedar, R., A. Kolecka, T. Boekhout, J.W. Fell, A. Anand, A. Al Malaki, A. Zeyara and M. Al Marri. 2018. Naganishia qatarensis sp. nov., a novel basidiomycetous yeast species from a hypersaline marine environment in Qatar. Int. J. Syst. Evol. Microbiol. 68: 2924–2929.
- Export Citation
Fotedar, R., A. Kolecka, T. Boekhout, J.W. Fell, A. Anand, A. Al Malaki, A. Zeyara and M. Al Marri. 2018.)| false Naganishia qatarensissp. nov., a novel basidiomycetous yeast species from a hypersaline marine environment in Qatar. Int. J. Syst. Evol. Microbiol. 68: 2924–2929. 10.1099/ijsem.0.002920 30070622
Frölich-Nowoisky, J., S.M. Burrows, Z. Xie, G. Engling, P.A. Solomon, M.P. Fraser, O.L. Mayol-Bracero, P. Artaxo, D. Begerow, R. Conrad, M.O. Andreae, V.R. Després and U. Pöschl. 2012. Biogeography in the air: fungal diversity over land and oceans. Biogeoscience 9: 1125–1136.
- Export Citation
Frölich-Nowoisky, J., S.M. Burrows, Z. Xie, G. Engling, P.A. Solomon, M.P. Fraser, O.L. Mayol-Bracero, P. Artaxo, D. Begerow, R. Conrad, M.O. Andreae, V.R. Després and U. Pöschl. 2012. Biogeography in the air: fungal diversity over land and oceans.)| false Biogeoscience 9: 1125–1136. 10.5194/bg-9-1125-2012
Glass, E., J. Wilkening, A. Wilke, D. Antonopoulos and F. Meyer. 2010. Using the metagenomics RAST Server (MG-RAST) for analyzing shotgun metagenomes. Cold Spring Harb. Protoc. 1: pdb.prot5368.
Gnavi, G., A. Poli, V. Prigione, G. Burgaud and G.C. Varese. 2017. The culturable mycobiota of Flabellia petiolata: first survey of marine fungi associated to a Mediterranean green alga. PLoS One 12: 1–20.
Good, S.A., M.J. Martin and N.A. Rayner. 2013. EN4: quality controlled ocean temperature and salinity profiles and monthly objective analyses with uncertainty estimates. J. Geophys. Res. Oceans 118: 6704–6716.
Grolig, F., M. Döring and P. Galland. 2006. Gravisusception by buoyancy: a mechanism ubiquitous among fungi? Protoplasma 229: 117–123.
Grossart, H.-P., C. Wurzbacher, T.Y. James and M. Kagami. 2016. Discovery of dark matter fungi in aquatic ecosystems demands a reappraisal of the phylogeny and ecology of zoosporic fungi. Fungal Ecol. 19: 28–38.
Gutiérrez, M.H., S. Pantoja, E. Tejos and R.A. Quiñones. 2011. The role of fungi in processing marine organic matter in the upwelling ecosystem off Chile. Mar. Biol. 158: 205–219.
Gutiérrez, M., A.M. Jara and S. Pantoja. 2016. Fungal parasites infect marine diatoms in the upwelling ecosystem of the Humboldt Current system off central Chile. Environ. Microbiol. 18: 1646–1653.
Hamm, C.E., R. Merkel, O. Springer, P. Jurkojc, C. Maier, K. Prechtel and V. Smetacek. 2003. Architecture and material properties of diatom shells provide effective mechanical protection. Nature 421: 841–843.
Hassenrück, C. 2018. R and bash scripts for sequence processing of amplicon and shotgun sequencing data. https://github.com/chassenr/NGS.
Hassett, B.T. and R. Gradinger. 2016. Chytrids dominate Arctic marine fungal communities. Environ. Microbiol. 18: 2001–2009.
Hassett, B.T., A.L. Ducluzeau, R.E. Collins and R. Gradinger. 2017. Spatial distribution of aquatic marine fungi across the western Arctic and sub-Arctic. Environ. Microbiol. 19: 475–484.
Hassett, B.T., E.J. Borrego, T.R. Vonnahme, T. Rämä, M.V. Kolomiets and R. Gradinger. 2019. Arctic marine fungi: biomass, functional genes, and putative ecological roles. ISME J. 13: 1484–1496.
Hawksworth, D. and R. Lucking. 2017. Fungal diversity revisited: 2.2 to 3.8 million species. Microbiol. Spectr. 5. Doi:10.1128/microbiolspec.FUNK-0052–2016.
Heusser, L. 1988. Pollen distribution in marine sediments on the continental margin off northern California. Mar. Geol. 80: 131–147.
Höhnk, W. 1961. A further contribution to the oceanic mycology. Rapp. P.V. Réun. Coms. Inst. Explor. Mer. 149: 202–208.
Horner, R. and G.C. Schrader. 1982. Relative contributions of ice algae, phytoplankton, and benthic microaglae to primary production in nearshore regions of the Beaufort Sea. Arctic 35: 485–503.
Hovmøller, M.S., A.H. Yahyaoui, E.A. Milus and A.F. Justesen. 2008. Rapid global spread of two aggressive strains of a wheat rust fungus. Mol. Ecol. 17: 3818–3826.
James, T., F. Kauff, C.L. Schoch, P.B. Matheny, V. Hofstetter, C.J. Cox, G. Celio, C. Gueidan, E. Fraker, J. Miadlikowska, H.T. Lumbsch, A. Rauhut, V. Reeb, A.E. Arnold, A. Amtoft, J.E. Stajich, K. Hosaka, G.H. Sung, D. Johnson, B. O’Rourke, M. Crockett, M. Binder, J.M. Curtis, J.C. Slot, Z. Wang, A.W. Wilson, A. Schüssler, J.E. Longcore, K. O’Donnell, S. Mozley-Standridge, D. Porter, P.M. Letcher, M.J. Powell, J.W. Taylor, M.M. White, G.W. Griffith, D.R. Davies, R.A. Humber, J.B. Morton, J. Sugiyama, A.Y. Rossman, J.D. Rogers, D.H. Pfister, D. Hewitt, K. Hansen, S. Hambleton, R.A. Shoemaker, J. Kohlmeyer, B. Volkmann-Kohlmeyer, R.A. Spotts, M. Serdani, P.W. Crous, K.W. Hughes, K. Matsuura, E. Langer, G. Langer, W.A. Untereiner, R. Lücking, B. Büdel, D.M. Geiser, A. Aptroot, P. Diederich, I. Schmitt, M. Schultz, R. Yahr, D.S. Hibbett, F. Lutzoni, D.J. McLaughlin, J.W. Spatafora and R. Vilgalys. 2006. Reconstructing the early evolution of Fungi using a six-gene phylogeny. Nature 443: 818–822.
- Export Citation
James, T., F. Kauff, C.L. Schoch, P.B. Matheny, V. Hofstetter, C.J. Cox, G. Celio, C. Gueidan, E. Fraker, J. Miadlikowska, H.T. Lumbsch, A. Rauhut, V. Reeb, A.E. Arnold, A. Amtoft, J.E. Stajich, K. Hosaka, G.H. Sung, D. Johnson, B. O’Rourke, M. Crockett, M. Binder, J.M. Curtis, J.C. Slot, Z. Wang, A.W. Wilson, A. Schüssler, J.E. Longcore, K. O’Donnell, S. Mozley-Standridge, D. Porter, P.M. Letcher, M.J. Powell, J.W. Taylor, M.M. White, G.W. Griffith, D.R. Davies, R.A. Humber, J.B. Morton, J. Sugiyama, A.Y. Rossman, J.D. Rogers, D.H. Pfister, D. Hewitt, K. Hansen, S. Hambleton, R.A. Shoemaker, J. Kohlmeyer, B. Volkmann-Kohlmeyer, R.A. Spotts, M. Serdani, P.W. Crous, K.W. Hughes, K. Matsuura, E. Langer, G. Langer, W.A. Untereiner, R. Lücking, B. Büdel, D.M. Geiser, A. Aptroot, P. Diederich, I. Schmitt, M. Schultz, R. Yahr, D.S. Hibbett, F. Lutzoni, D.J. McLaughlin, J.W. Spatafora and R. Vilgalys. 2006. Reconstructing the early evolution of Fungi using a six-gene phylogeny.)| false Nature 443: 818–822. 10.1038/nature05110 17051209
Jebaraj, C., C. Raghukumar, A. Behnke and T. Stoeck. 2010. Fungal diversity in oxygen-depleted regions of the Arabian Sea revealed by targeted environmental sequencing combined with cultivation. FEMS Microbiol. Ecol. 71: 399–412.
- Export Citation
Jebaraj, C., C. Raghukumar, A. Behnke and T. Stoeck. 2010. Fungal diversity in oxygen-depleted regions of the Arabian Sea revealed by targeted environmental sequencing combined with cultivation.)| false FEMS Microbiol. Ecol. 71: 399–412. 10.1111/j.1574-6941.2009.00804.x 20002178
Jeffries, T.C., N.J. Curlevski, M.V. Brown, D.P. Harrison, M.A. Doblin, K. Petrou, P.J. Ralph and J.R. Seymour. 2016. Partitioning of fungal assemblages across different marine habitats. Environ. Microbiol. Rep. 8: 235–238.
Johnson, G.C. and M.J. McPhaden. 2001. Equatorial Pacific Ocean horizontal velocity, divergence, and upwelling. J. Phys. Oceanogr. 31: 839–849.
Johnson, T. and F.K. Sparrow. 1961. Fungi in oceans and estuaries. J. Cramer, Germany. pp. 668.
Jones, E.B.G. 1994. Fungal adhesion. Presidential address 1992. Mycol. Res. 98: 961–981.
Jones, E.B.G. and T. Le Campion-Alsumard. 1970. Marine fungi on polyurethane plates submerged in the sea. Nova Hedw. 19: 567–590.
Jones, E.B.G. and K.L. Pang. 2012b. Marine fungi and fungi-like organisms. Walter de Gruyter GmbH & Co. KG, Berlin/Boston.
Jones, E.B.G., S. Suetrong, J. Sakayaroj, A.H. Bahkali, M.A. Abdel-Wahab, T. Boekhout and K.L. Pang. 2015. Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota. Fungal Divers. 73: 1–72.
Jones, E.B.G., K.L. Pang, M. Abdel-Wahab, B. Scholz, K.D. Hyde, T. Boekhout, R. Ebel, M.E. Rateb, L. Henderson, J. Sakayaroj, S. Suetrong, M.C. Dayarathne, V. Kumar, S. Raghukumar, K.R. Sridhar, A.H.A. Bahkali, F. Gleason and C. Norphanphoun. 2019. An online resource for marine fungi. Fungal Divers.https://doi.org/10.1007/s13225-019-00426-5.
Karpov, S.A., D. Moreira, M.A. Mamkaeva, O.V. Popova, V.V. Aleoshin and P. López-García. 2018. New member of Gromochytriales (Chytridiomycetes) – Apiochytrium granulosporum nov. gen. et sp. J. Eukaryot. Microbiol.https://doi.org/10.1111/jeu.12702.
Kazama, F. 1972. Ultrastructure and phototaxis of the zoospores of Phlyctochytrium sp., and estuarine chytrid. Microbiology 71: 555–566.
Kivlin, S., C.V. Hawkes and K.K. Treseder. 2011. Global diversity and distribution of arbuscular mycorrhizal fungi. Soil Biol. Biochem. 43: 2294–2303.
Kohlmeyer, J. and E. Kohlmeyer. 1979. Marine mycology: the higher fungi. Academic Press, New York, New York, USA. pp. 690.
Kopf, A., M. Bicak, R. Kottmann, J. Schnetzer, I. Kostadinov, K. Lehmann, A. Fernandez-Guerra, C. Jeanthon, E. Rahav, M. Ullrich, A. Wichels, G. Gerdts, P. Polymenakou, G. Kotoulas, R. Siam, R.Z. Abdallah, E.C. Sonnenschein, T. Cariou, F. O’Gara, S. Jackson, S. Orlic, M. Steinke, J. Busch, B. Duarte, I. Caçador, J. Canning-Clode, O. Bobrova, V. Marteinsson, E. Reynisson, C.M. Loureiro, G.M. Luna, G.M. Quero, C.R. Löscher, A. Kremp, M.E. DeLorenzo, L. Øvreås, J. Tolman, J. LaRoche, A. Penna, M. Frischer, T. Davis, B. Katherine, C.P. Meyer, S. Ramos, C. Magalhães, F. Jude-Lemeilleur, M.L. Aguirre-Macedo, S. Wang, N. Poulton, S. Jones, R. Collin, J.A. Fuhrman, P. Conan, C. Alonso, N. Stambler, K. Goodwin, M.M. Yakimov, F. Baltar, L. Bodrossy, J. Van De Kamp, D.M. Frampton, M. Ostrowski, P. Van Ruth, P. Malthouse, S. Claus, K. Deneudt, J. Mortelmans, S. Pitois, D. Wallom, I. Salter, R. Costa, D.C. Schroeder, M.M. Kandil, V. Amaral, F. Biancalana, R. Santana, M.L. Pedrotti, T. Yoshida, H. Ogata, T. Ingleton, K. Munnik, N. Rodriguez-Ezpeleta, V. Berteaux-Lecellier, P. Wecker, I. Cancio, D. Vaulot, C. Bienhold, H. Ghazal, B. Chaouni, S. Essayeh, S. Ettamimi, E.H. Zaid, N. Boukhatem, A. Bouali, R. Chahboune, S. Barrijal, M. Timinouni, F. El Otmani, M. Bennani, M. Mea, N. Todorova, V. Karamfilov, P. Ten Hoopen, G. Cochrane, S. L’Haridon, K.C. Bizsel, A. Vezzi, F.M. Lauro, P. Martin, R.M. Jensen, J. Hinks, S. Gebbels, R. Rosselli, F. De Pascale, R. Schiavon, A. Dos Santos, E. Villar, S. Pesant, B. Cataletto, F. Malfatti, R. Edirisinghe, J.A. Silveira, M. Barbier, V. Turk, T. Tinta, W.J. Fuller, I. Salihoglu, N. Serakinci, M.C. Ergoren, E. Bresnan, J. Iriberri, P.A. Nyhus, E. Bente, H.E. Karlsen, P.N. Golyshin, J.M. Gasol, S. Moncheva, N. Dzhembekova, Z. Johnson, C.D. Sinigalliano, M.L. Gidley, A. Zingone, R. Danovaro, G. Tsiamis, M.S. Clark, A.C. Costa, M. El Bour, A.M. Martins, R.E. Collins, A.L. Ducluzeau, J. Martinez, M.J. Costello, L.A. Amaral-Zettler, J.A. Gilbert, N. Davies, D. Field and F.O. Glöckner. 2015. The ocean sampling day consortium. GigaScience 4: 1–5.
Kozich, J.J., S.L. Westcott, N.T. Baxter, S.K. Highlander and P.D. Schloss. 2013. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl. Environ. Microbiol. 79: 5112–5120.
- Export Citation
Kozich, J.J., S.L. Westcott, N.T. Baxter, S.K. Highlander and P.D. Schloss. 2013. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.)| false Appl. Environ. Microbiol. 79: 5112–5120. 10.1128/AEM.01043-13 23793624
Kriss, A.E. 1963. Marine microbiology. Oliver and Boyd, Edinburgh. pp. 536.
Lennon, J. and S.E. Jones. 2011. Microbial seed banks: the ecological and evolutionary implications of dormancy. Nat. Rev. Microbiol. 9: 119–130.
Lepelletier, F., S.A. Karpov, E. Alacid, S. Le Panse, E. Bigeard, E. Garcés, C. Jeanthon and L. Guillou. 2014. Dinomyces arenysensis gen. et sp. nov. (Rhizophydiales, Dinomycetaceae fam. nov.), a chytrid infecting marine dinoflagellates. Protist 165: 230–244.
- Export Citation
Lepelletier, F., S.A. Karpov, E. Alacid, S. Le Panse, E. Bigeard, E. Garcés, C. Jeanthon and L. Guillou. 2014.)| false Dinomyces arenysensisgen. et sp. nov. (Rhizophydiales, Dinomycetaceae fam. nov.), a chytrid infecting marine dinoflagellates. Protist 165: 230–244. 10.1016/j.protis.2014.02.004 24709472
Letcher, P.M., J.E. Longcore, C.A. Quandt, D.D. Leite, T.Y. James and M.J. Powell. 2017. Morphological, molecular, and ultrastructural characterization of Rozella rhizoclosmatii, a new species in Cryptomycota. Fungal Biol. 121: 1–10.
- Export Citation
Letcher, P.M., J.E. Longcore, C.A. Quandt, D.D. Leite, T.Y. James and M.J. Powell. 2017. Morphological, molecular, and ultrastructural characterization of)| false Rozella rhizoclosmatii, a new species in Cryptomycota. Fungal Biol. 121: 1–10. 28007212 10.1016/j.funbio.2016.08.008
Lin, Y., X. Wu, Z. Deng, J. Wang, S. Zhou, L.L.P. Vrijmoed and E.B.G. Jones. 2002. The metabolites of the mangrove fungus Verruculina enalia No 2606 from a salt lake in the Bahamas. Phytochemistry 59: 469–471.
Lindahl, B.D., R.H. Nilsson, L. Tedersoo, K. Abarenkov, T. Carlsen, R. Kjøller, U. Kõljalg, T. Pennanen, S. Rosendahl, J. Stenlid and H. Kauserud. 2013. Fungal community analysis by high-throughput sequencing of amplified markers – a user’s guide. New Phytol. 199: 288–299.
- Export Citation
Lindahl, B.D., R.H. Nilsson, L. Tedersoo, K. Abarenkov, T. Carlsen, R. Kjøller, U. Kõljalg, T. Pennanen, S. Rosendahl, J. Stenlid and H. Kauserud. 2013. Fungal community analysis by high-throughput sequencing of amplified markers – a user’s guide.)| false New Phytol. 199: 288–299. 23534863 10.1111/nph.12243
Livermore, J. and T.E. Mattes. 2013. Phylogenetic detection of novel Cryptomycota in an Iowa (United States) aquifer and from previously collected marine and freshwater targeted high-throughput sequencing sets. Environ. Microbiol. 15: 2333–2341.
Loque, C.P., A.O. Medeiros, F.M. Pellizzari, E.C. Oliveira, C.A. Rosa and L.H. Rosa. 2010. Fungal community associated with marine macroalgae from Antarctica. Polar Biol. 33: 641–648.
Marquardt, M., A. Vader, E.I. Stübner, M. Reigstad and T.M. Gabrielsen. 2016. Strong seasonality of marine microbial eukaryotes in a high-Arctic fjord (Isfjorden, in West Spitsbergen, Norway). Appl. Environ. Microbiol. 82: 1868–1880.
McCoy, E. and K.L. Heck. 1976. Biogeography of corals, seagrasses, and mangroves: an alternative to the center of origin concept. Syst. Biol. 25: 201–210.
Menge, B. and J.P. Sutherland. 1976. Species diversity gradients: synthesis of the roles of predation, competition, and temporal heterogeneity. Am. Nat. 110: 351–369.
Meyers, S. and E.S. Reynolds. 1958. A wood incubation method for the study of lignicolous marine fungi. Bull. Marin. Sci. Gulf Caribbean 8: 342–347.
Meyers, S., D.G. Ahearn and F.J. Roth. 1967b. Mycological investigation of Black Sea. Bull. Mar. Sci. 17: 576–596.
Mileikovsky, S. 1971. Types of larval development in marine bottom invertebrates, their distribution and ecological significance: a re-evaluation. Marine Biol. 10: 193–213.
Mohamed, D.J. and J.B.H. Martiny. 2011. Patterns of fungal diversity and composition along a salinity gradient. ISME J. 5: 379–388.
Morales, S.E., A. Biswas, G.J. Herndl and F. Balter. 2019. Global structuring of phylogenetic and functional diversity of pelagic fungi by depth and temperature. Front. Mar. Sci. 6: 131.
Morrison-Gardiner, S. 2002. Dominant fungi from Australian coral reefs. Fungal Divers. 9: 105–121.
Muehlstein, L., J.P. Amon and D.L. Leffler. 1988. Chemotaxis in the marine fungus, Rhizophydium littoreum. Appl. Environ. Microbiol. 54: 1668–1672.
Nagahama, T. 2006. Yeast biodiversity in freshwater, marine and deep-sea environments. In: (G. Péter and C. Rosa, eds.) Biodiversity and ecophysiology of yeasts. Springer Berlin, Heidelberg. pp. 241–262.
Nagai, K. 2002. YM-202204, a new antifungal antibiotic produced by marine fungus Phoma sp. J. Antibiot. 55: 1036–1041.
Nagano, Y., T. Miura, S. Nishi, A.O. Lima, N. Cristina, V.H. Pellizari and K. Fujikura. 2017. Fungal diversity in deep-sea sediments associated with asphalt seeps in the Sao Paulo Plateau. Deep Sea Res. II Top. Stud. Oceanogr. 146: 59–67.
Nakagiri, A. 1989. Marine fungi in sea foam from Japanese coast. IFO Res. Commun. 14: 52–79.
Nilson, R.H., S. Anslan, M. Bahram, C. Wurzbacher, P. Baldrian and L. Tedersoo. 2018. Mycobiome diversity: high-throughput sequencing and identification of fungi. Nat. Rev. Microbiol. 17: 95–109.
Orsi, W., F.J. Biddle and V. Edgcomb. 2013. Deep sequencing of subseafloor eukaryotic rRNA reveals active fungi across marine subsurface provinces. PLoS One 8: e56335.
Ortega-Arbulú, A.S., M. Pichler, A. Vuillemin and W.D. Orsi. 2018. Effects of organic matter and low oxygen on the mycobenthos in a coastal lagoon. Environ. Microbiol. 21: 374–388.
Pan, J.H., E.B.G. Jones, Z.G. She, J.Y. Pang and Y.C. Lin. 2008. Review of bioactive compounds from fungi in the South China Sea. Bot. Mar. 51: 179–190.
Pang, K.L., J.S. Jheng and E.B.G Jones. 2011. Marine mangrove fungi of Taiwan. National Taiwan Ocean University Press, Keelung. pp. 1–131.
Pang, K.L., D.P. Overy, E.B.G. Jones, M. da Luz Calado, G. Burgaud, A.K. Walker, J.A. Johnson, R.G. Kerr, H.J. Cha and G.F. Bills. 2016. Marine fungi and marine-derived fungi in natural product chemistry research: toward a new consensual definition. Fungal Biol. Rev. 30: 163–175.
- Export Citation
Pang, K.L., D.P. Overy, E.B.G. Jones, M. da Luz Calado, G. Burgaud, A.K. Walker, J.A. Johnson, R.G. Kerr, H.J. Cha and G.F. Bills. 2016. Marine fungi and marine-derived fungi in natural product chemistry research: toward a new consensual definition.)| false Fungal Biol. Rev. 30: 163–175. 10.1016/j.fbr.2016.08.001
Panzer, K., P. Yilmaz, M. Weiß, L. Reich, M. Richter, J. Wiese, R. Schmaljohann, A. Labes, J.F. Imhoff, F.O. Glöckner and M. Reich. 2015. Identification of habitat-specific biomes of aquatic fungal communities using a comprehensive nearly full-length 18S rRNA dataset enriched with contextual data. PLoS One 10: e0134377.
- Export Citation
Panzer, K., P. Yilmaz, M. Weiß, L. Reich, M. Richter, J. Wiese, R. Schmaljohann, A. Labes, J.F. Imhoff, F.O. Glöckner and M. Reich. 2015. Identification of habitat-specific biomes of aquatic fungal communities using a comprehensive nearly full-length 18S rRNA dataset enriched with contextual data.)| false PLoS One 10: e0134377. 26226014 10.1371/journal.pone.0134377
Pearman, J.K., J. Ellis, X. Irigoien, Y.V.B. Sarma, B.H. Jones and S. Carvalho. 2017. Microbial planktonic communities in the Red Sea: high levels of spatial and temporal variability shaped by nutrient availability and turbulence. Sci. Rep. 7: 6611.
- Export Citation
Pearman, J.K., J. Ellis, X. Irigoien, Y.V.B. Sarma, B.H. Jones and S. Carvalho. 2017. Microbial planktonic communities in the Red Sea: high levels of spatial and temporal variability shaped by nutrient availability and turbulence.)| false Sci. Rep. 7: 6611. 10.1038/s41598-017-06928-z 28747798
Pesant, S., F. Not, M. Picheral, S. Kandels-Lewis, N. Le Bescot, G. Gorsky, D. Iudicone, E. Karsenti, S. Speich, R. Troublé, C. Dimier, S. Searson and Tara Oceans Consortium Coordinators. 2015. Open science resources for the discovery and analysis of Tara Oceans data. Sci. Data 2: 150023.
- Export Citation
Pesant, S., F. Not, M. Picheral, S. Kandels-Lewis, N. Le Bescot, G. Gorsky, D. Iudicone, E. Karsenti, S. Speich, R. Troublé, C. Dimier, S. Searson and Tara Oceans Consortium Coordinators. 2015. Open science resources for the discovery and analysis of Tara Oceans data.)| false Sci. Data 2: 150023. 26029378 10.1038/sdata.2015.23
Phillips, J. 2001. Marine macroalgal biodiversity hotspots: why is there high species richness and endemism in southern Australian marine benthic flora? Biodivers. Conserv. 10: 1555–1577.
Pivkin, M. 2000. Filamentous fungi associated with holothurians from the Sea of Japan, off the Primorye Coast of Russia. Biol. Bull. 198: 101–109.
Powell, M. 1993. Looking at mycology with a janus face: a glimpse at Chytridiomycetes active in the environment. Mycologia 85: 1–20.
Proksch, P., R. Ebel, R. Edrada, F. Riebe, H. Liu, A. Diesel, M. Bayer, X. Li, W. Han Lin, V. Grebenyuk, W.E.G. Müller, S. Draeger, A. Zuccaro and B. Schulz. 2008. Sponge-associated fungi and their bioactive compounds: the Suberites case. Bot. Mar. 51: 209–218.
Raghukumar, S. 2017. Fungi in coastal and oceanic marine ecosystems. Springer, Cham, Switzerland. pp. 378.
Rämä, T., M.L. Davey, J. Nordén, R. Halvorsen, R. Blaalid, G.H. Mathiassen, I.G. Alsos and H. Kauserud. 2016. Fungi sailing the Arctic Ocean: speciose communities in North Atlantic driftwood as revealed by high-throughput amplicon sequencing. Microb. Ecol. 72: 295–304.
- Export Citation
Rämä, T., M.L. Davey, J. Nordén, R. Halvorsen, R. Blaalid, G.H. Mathiassen, I.G. Alsos and H. Kauserud. 2016. Fungi sailing the Arctic Ocean: speciose communities in North Atlantic driftwood as revealed by high-throughput amplicon sequencing.)| false Microb. Ecol. 72: 295–304. 10.1007/s00248-016-0778-9 27147245
Rämä, T., B.T. Hassett and K. Bubnova. 2017. Arctic marine fungi: from filaments and flagella to operational taxonomic units and beyond. Bot. Mar. 60: 433–452.
Richards, T.A., M.D.M. Jones, G. Leonard and D. Bass. 2012. Marine fungi: their ecology and molecular diversity. Annu. Rev. Mar. Sci. 4: 495–522.
Rohde, K. 1992. Latitudinal gradients in species diversity: the search for the primary cause. Oikos 65: 541–527.
Rojas-Jimenez, K., A. Rieck, C. Wurzbacher, K. Jürgens, M. Labrenz and H.P. Grossart. 2019. A salinity threshold separating fungal communities in the Baltic Sea. Front. Microbiol. 10: 680.
Roth, F.J. Jr., D.G. Ahearn, J.W. Fell, S.P. Meyers and S.A. Meyer. 1962. Ecology and taxonomy of yeasts from various marine substrates. Limnol. Oceanog. 7: 178–185.
Rusch, D.B., A.L. Halpern, G. Sutton, K.B. Heidelberg, S. Williamson, S. Yooseph, D. Wu, J.A. Eisen, J.M. Hoffman, K. Remington, K. Beeson, B. Tran, H. Smith, H. Baden-Tillson, C. Stewart, J. Thorpe, J. Freeman, C. Andrews-Pfannkoch, J.E. Venter, K. Li, S. Kravitz, J.F. Heidelberg, T. Utterback, Y.H. Rogers, L.I. Falcón, V. Souza, G. Bonilla-Rosso, L.E. Eguiarte, D.M. Karl, S. Sathyendranath, T. Platt, E. Bermingham, V. Gallardo, G. Tamayo-Castillo, M.R. Ferrari, R.L. Strausberg, K. Nealson, R. Friedman, M. Frazier and J.C. Venter. 2007. The Sorcerer II global ocean sampling expedition: northwest Atlantic through eastern tropical pacific. PLoS Biol. 5: e77.
- Export Citation
Rusch, D.B., A.L. Halpern, G. Sutton, K.B. Heidelberg, S. Williamson, S. Yooseph, D. Wu, J.A. Eisen, J.M. Hoffman, K. Remington, K. Beeson, B. Tran, H. Smith, H. Baden-Tillson, C. Stewart, J. Thorpe, J. Freeman, C. Andrews-Pfannkoch, J.E. Venter, K. Li, S. Kravitz, J.F. Heidelberg, T. Utterback, Y.H. Rogers, L.I. Falcón, V. Souza, G. Bonilla-Rosso, L.E. Eguiarte, D.M. Karl, S. Sathyendranath, T. Platt, E. Bermingham, V. Gallardo, G. Tamayo-Castillo, M.R. Ferrari, R.L. Strausberg, K. Nealson, R. Friedman, M. Frazier and J.C. Venter. 2007. The Sorcerer II global ocean sampling expedition: northwest Atlantic through eastern tropical pacific.)| false PLoS Biol. 5: e77. 10.1371/journal.pbio.0050077 17355176
Saritha, N., G. Valerie and N. Shweta. 2012. Isolation and salt tolerance of halophilic fungi from mangroves and solar salterns, Goa, India. Indian J. Microbiol. 52: 22–27.
Schoch, C., K.A. Seifert, S. Huhndorf, V. Robert, J.L. Spouge, C.A. Levesque, W. Chen, Fungal Barcoding Consortium. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. PNAS 109: 6241–6246.
- Export Citation
Schoch, C., K.A. Seifert, S. Huhndorf, V. Robert, J.L. Spouge, C.A. Levesque, W. Chen, Fungal Barcoding Consortium. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi.)| false PNAS 109: 6241–6246. 10.1073/pnas.1117018109 22454494
Schulz, B., S. Draeger, T.E. dela Cruz, J. Rheinheimer, K. Siems, S. Loesgen, J. Bitzer, O. Schloerke, A. Zeeck, I. Kock, H. Hussain, J. Dai and K. Krohn. 2008. Screening strategies for obtaining novel, biologically active, fungal secondary metabolites from marine habitats. Bot. Mar. 51: 218–234.
Seto, K. and Y. Degawa. 2015. Cyclopsomyces plurioperculatus: a new genus and species of Lobulomycetales (Chytrdiomycota, Chytridiomycetes) from Japan. Mycologia 107: 633–640.
Singh, S.M., M. Tsuji, P. Gawas-Sakhalker, M.J.J.E. Loonen and T. Hoshino. 2016. Bird feather fungi from Svalbard Arctic. Polar Biol. 39: 523–532.
Sivaramanan, S. 2014. Culturing cellulolytic fungi in sea water. Int. J. Sci. Res. Publ. 4: 1–6.
Sofianos, S., W.E. Johns and S.P. Murray. 2002. Heat and freshwater budgets in the Red Sea from direct observations at Bab el Mandeb. Deep Sea Res. Part II: Top. Stud. Oceanogr. 49: 1323–1340.
Soininem, J., J.J. Korhonen and M. Luoto. 2013. Stochastic species distributions are driven by organism size. Ecology 94: 660–670.
Spatafora, J., B. Volkmann-Kohlmeyer and J. Kohlmeyer. 1998. Independent terrestria lorigins of the Halosphaeriales (marine Ascomycota). Am. J. Bot. 85: 1569–1580.
Stern, R.F., K.T. Picard, K.M. Hamilton, A. Walne, G.A. Tarran, D. Mills, A. McQuatters-Gollop and M. Edwards. 2015. Novel lineage patterns from an automated water sampler to probe marine microbial biodiversity with ships of opportunity. Prog. Oceanogr. 137: 409–420.
- Export Citation
Stern, R.F., K.T. Picard, K.M. Hamilton, A. Walne, G.A. Tarran, D. Mills, A. McQuatters-Gollop and M. Edwards. 2015. Novel lineage patterns from an automated water sampler to probe marine microbial biodiversity with ships of opportunity.)| false Prog. Oceanogr. 137: 409–420. 10.1016/j.pocean.2015.04.015
Stevens, G. 1989. The latitudinal gradient in geographical range: how so many species coexist in the tropics. Am. Nat. 133: 240–256.
Sunagawa, S., L.P. Coelho, S. Chaffron, J.R. Kultima, K. Labadie, G. Salazar, B. Djahanschiri, G. Zeller, D.R. Mende, A. Alberti, F.M. Cornejo-Castillo, P.I. Costea, C. Cruaud, F. D’Ovidio, S. Engelen, I. Ferrera, J.M. Gasol, L. Guidi, F. Hildebrand, F. Kokoszka, C. Lepoivre, G. Lima-Mendez, J. Poulain, B.T. Poulos, M. Royo-Llonch, H. Sarmento, S. Vieira-Silva, C. Dimier, M. Picheral, S. Searson, S. Kandels-Lewis, E. Boss, M. Follows, L. Karp-Boss, U. Krzic, E.G. Reynaud, C. Sardet, M. Sieracki, D. Velayoudon, C. Bowler, C. De Vargas, G. Gorsky, N. Grimsley, P. Hingamp, D. Iudicone, O. Jaillon, F. Not, H. Ogata, S. Pesant, S. Speich, L. Stemmann, M. Sullivan, J. Weissenbach, P. Wincker, E. Karsenti, J. Raes, S.G. Acinas and P. Bork. 2015. Structure and function of the global ocean microbiome. Science 348: 1261359.
- Export Citation
Sunagawa, S., L.P. Coelho, S. Chaffron, J.R. Kultima, K. Labadie, G. Salazar, B. Djahanschiri, G. Zeller, D.R. Mende, A. Alberti, F.M. Cornejo-Castillo, P.I. Costea, C. Cruaud, F. D’Ovidio, S. Engelen, I. Ferrera, J.M. Gasol, L. Guidi, F. Hildebrand, F. Kokoszka, C. Lepoivre, G. Lima-Mendez, J. Poulain, B.T. Poulos, M. Royo-Llonch, H. Sarmento, S. Vieira-Silva, C. Dimier, M. Picheral, S. Searson, S. Kandels-Lewis, E. Boss, M. Follows, L. Karp-Boss, U. Krzic, E.G. Reynaud, C. Sardet, M. Sieracki, D. Velayoudon, C. Bowler, C. De Vargas, G. Gorsky, N. Grimsley, P. Hingamp, D. Iudicone, O. Jaillon, F. Not, H. Ogata, S. Pesant, S. Speich, L. Stemmann, M. Sullivan, J. Weissenbach, P. Wincker, E. Karsenti, J. Raes, S.G. Acinas and P. Bork. 2015. Structure and function of the global ocean microbiome.)| false Science 348: 1261359. 10.1126/science.1261359 25999513
Taylor, J. and M. Cunliffe. 2016. Multi-year assessment of coastal planktonic fungi reveals environmental drivers of diversity and abundance. ISME J. 10: 2118–2128.
Tedersoo, L., T.W. May and M.E. Smith. 2010. Ectomycorrhizal lifestyle in fungi: global diversity, distribution, and evolution of phylogenetic lineages. Mycorrhiza 20: 217–263.
Tedersoo, L., M. Bahram, S. Põlme, U. Kõljalg, N.S. Yorou, R. Wijesundera, R.L. Villarreal, A.M. Vasco-Palacios, P.Q. Thu, A. Suija, M.E. Smith, C. Sharp, E. Saluveer, A. Saitta, M. Rosas, T. Riit, D. Ratkowsky, K. Pritsch, K. Põldmaa, M. Piepenbring, C. Phosri, M. Peterson, K. Parts, K. Pärtel, E. Otsing, E. Nouhra, A.L. Njouonkou, R.H. Nilsson, L.N. Morgado, J. Mayor, T.W. May, L. Majuakim, D.J. Lodge, S.S. Lee, K.H. Larsson, P. Kohout, K. Hosaka, I. Hiiesalu, T.W. Henkel, H. Harend, L.D. Guo, A. Greslebin, G. Grelet, J. Geml, G. Gates, W. Dunstan, C. Dunk, R. Drenkhan, J. Dearnaley, A. De Kesel, T. Dang, X. Chen, F. Buegger, F.Q. Brearley, G. Bonito, S. Anslan, S. Abell and K. Abarenkov. 2014. Global diversity and geography of soil fungi. Science 346: 1256688.
- Export Citation
Tedersoo, L., M. Bahram, S. Põlme, U. Kõljalg, N.S. Yorou, R. Wijesundera, R.L. Villarreal, A.M. Vasco-Palacios, P.Q. Thu, A. Suija, M.E. Smith, C. Sharp, E. Saluveer, A. Saitta, M. Rosas, T. Riit, D. Ratkowsky, K. Pritsch, K. Põldmaa, M. Piepenbring, C. Phosri, M. Peterson, K. Parts, K. Pärtel, E. Otsing, E. Nouhra, A.L. Njouonkou, R.H. Nilsson, L.N. Morgado, J. Mayor, T.W. May, L. Majuakim, D.J. Lodge, S.S. Lee, K.H. Larsson, P. Kohout, K. Hosaka, I. Hiiesalu, T.W. Henkel, H. Harend, L.D. Guo, A. Greslebin, G. Grelet, J. Geml, G. Gates, W. Dunstan, C. Dunk, R. Drenkhan, J. Dearnaley, A. De Kesel, T. Dang, X. Chen, F. Buegger, F.Q. Brearley, G. Bonito, S. Anslan, S. Abell and K. Abarenkov. 2014. Global diversity and geography of soil fungi.)| false Science 346: 1256688. 25430773 10.1126/science.1256688
Tedersoo, L., S. Anslan, M. Bahram, S. Põlme, T. Riit, I. Liiv, U. Kõljalg, V. Kisand, H. Nilsson, F. Hildebrand, P. Bork and K. Abarenkov. 2015. Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi. MycoKeys 10: 1–43.
- Export Citation
Tedersoo, L., S. Anslan, M. Bahram, S. Põlme, T. Riit, I. Liiv, U. Kõljalg, V. Kisand, H. Nilsson, F. Hildebrand, P. Bork and K. Abarenkov. 2015. Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi.)| false MycoKeys 10: 1–43. 10.3897/mycokeys.10.4852
Tedersoo, L., S. Sánchez-Ramírez, U. Kõljalg, M. Bahram, M. Döring, D. Schigel, T. May, M. Ryberg and K. Abarenkov. 2018. High-level classification of the fungi and a tool for evolutionary ecological analyses. Fungal Divers. 90: 135–159.
Tisthammer, K., G.M. Cobian and A.S. Amend. 2016. Global biogeography of marine fungi is shaped by the environment. Fungal Ecol. 19: 39–46.
Tokura, R., Y. Shimooka, K. Moriguchi, T. Yagi, J. Nakanishi and K. Nakagawa. 1982. Studies on the proper guidance of biological marine practice. VI. Observation of the marine fungi in Hakoishi coastal region of Japan Sea. II. Annu. Rep. Res. Sci. Ed. Kyoto Univ. Ed. 12: 29–57.
Townsend, C., M.R. Scarsbrook and S. Dolédec. 2003. The intermediate disturbance hypothesis, refugia, and biodiversity in streams. Limnol. Oceanogr. 42: 938–949.
Van den Wyngaert, S., K. Rojas-Jimenez, K. Seto, M. Kagami and H.P. Grossart. 2018. Diversity and hidden host specificity of chytrids infecting colonial volvocacean algae. J. Eukaryot. Microbiol. 65: 870–881.
Van Uden, N. and C.E. Zobell. 1962. Candida marina nov. spec., Torulopsis torresii nov. spec. and T. maris nov. spec., three yeasts from the Torres Strait. Antonie Van Leeuwenhoek 28: 275–283.
Villarino, E., J.R. Watson, B. Jönsson, J.M. Gasol, G. Salazar, S.G. Acinas, M. Estrada, R. Massana, R. Logares, C.R. Giner, M.C. Pernice, M.P. Olivar, L. Citores, J. Corell, N. Rodríguez-Ezpeleta, J.L. Acuña, A. Molina-Ramírez, J.I. González-Gordillo, A. Cózar, E. Martí, J.A. Cuesta, S. Agustí, E. Fraile-Nuez, C.M. Duarte, X. Irigoien and G. Chust. 2018. Large-scale ocean connectivity and planktonic body size. Nat. Commun. 9: 142.
- Export Citation
Villarino, E., J.R. Watson, B. Jönsson, J.M. Gasol, G. Salazar, S.G. Acinas, M. Estrada, R. Massana, R. Logares, C.R. Giner, M.C. Pernice, M.P. Olivar, L. Citores, J. Corell, N. Rodríguez-Ezpeleta, J.L. Acuña, A. Molina-Ramírez, J.I. González-Gordillo, A. Cózar, E. Martí, J.A. Cuesta, S. Agustí, E. Fraile-Nuez, C.M. Duarte, X. Irigoien and G. Chust. 2018. Large-scale ocean connectivity and planktonic body size.)| false Nat. Commun. 9: 142. 29321528 10.1038/s41467-017-02535-8
Wang, X., P. Singh, Z. Gao, X. Zhang, Z.I. Johnson and G. Wang. 2014. Distribution and diversity of planktonic fungi in the west Pacific Warm Pool. PLoS One 9: e101523.
Wiens, J. and M.J. Donoghue. 2004. Historical biogeography, ecology and species richness. Trends Ecol. Evolut. 19: 639–644.
Worden, A.Z., M.J. Follows, S.J. Giovannoni, S. Wilken, A.E. Zimmerman and P.J. Keeling. 2015. Rethinking the marine carbon cycle: factoring in the multifarious lifestyles of microbes. Science 347: 1257594.
Yao, Q., J. Wang, X. Zhang, X. Nong, X. Xu and S. Qi. 2014. Cytotoxic polyketides from the deep-sea-derived fungus Engyodontium album DFFSCS021. Mar. Drugs 12: 5902–5915.
Yergeau, E., C. Maynard, S. Sanschagrin, J. Champagne, D. Juck, K. Lee and C.W. Greer. 2015. Microbial community composition, functions, and activities in the Gulf of Mexico 1 year after the Deepwater Horizon Accident. Appl. Environ. Microbiol. 81: 5855–5866.
- Export Citation
Yergeau, E., C. Maynard, S. Sanschagrin, J. Champagne, D. Juck, K. Lee and C.W. Greer. 2015. Microbial community composition, functions, and activities in the Gulf of Mexico 1 year after the Deepwater Horizon Accident.)| false Appl. Environ. Microbiol. 81: 5855–5866. 26092461 10.1128/AEM.01470-15
Zheng, J., H. Zhu, K. Hong, Y. Wang, P. Liu, X. Wang, X. Peng and W. Zhu. 2009. Novel cyclic hexapeptides from marine-derived fungus, Aspergillus sclerotiorum PT06-1. Org. Lett. 11: 5262–5265.
Zheng, J., Y. Wang, J. Wang, P. Liu, J. Li and W. Zhu. 2013. Antimicrobial ergosteroids and pyrrole derivatives from halotolerant Aspergillus flocculosus PT05-1 cultured in a hypersaline medium. Extremophiles 17: 963–971.
Zuccaro, A., R.C. Summerbell, W. Gams, H.-J. Schroers and J.I. Mitchell. 2004. A new Acremonium species associated with Fucus spp, and its affinity with a phylogenetically distinct marine Emericellopsis clade. Stud. Mycol. 50: 283–297.
Zuluaga-Montero, A., L. Ramírez-Camejo, J. Rauscher and P. Bayman. 2010. Marine isolates of Aspergillus flavus: denizens of the deep or lost at sea? Fungal Ecol. 3: 386–391.
The online version of this article offers supplementary material (