Establishment of detailed reference values for luteinizing hormone, follicle stimulating hormone, estradiol, and progesterone during different phases of the menstrual cycle on the Abbott ARCHITECT® analyzer

Reto Stricker 1. , 1. , Raphael Eberhart 2. , 2. , Marie-Christine Chevailler 3. , 3. , Frank A. Quinn 4. , 4. , Paul Bischof 5. , 5. ,  and René Stricker 6. , 6.
  • 1. DIANALAB SA, Geneva, Switzerland
  • 2. DIANALAB SA, Geneva, Switzerland
  • 3. DIANALAB SA, Geneva, Switzerland
  • 4. Abbott Diagnostics, Abbott Park, IL, United States of America
  • 5. Laboratoire d'Hormonologie Maternité, Department of Obstetrics and Gynaecology, University of Geneva, Geneva, Switzerland
  • 6. DIANALAB SA, Geneva, Switzerland

Abstract

During a normal menstrual cycle, serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, and progesterone can vary widely between cycles for the same woman, as well as between different woman. Reliable reference values based on the local population are important for correct interpretation of laboratory results. The purpose of our study was to determine detailed reference values for these hormones throughout the menstrual cycle using the Abbott ARCHITECT system. From 20 volunteers (age 20–36years) with normal cycles and no use of oral contraceptives, samples were taken every day during their cycle. Volunteers received three vaginal ultrasound examinations (days 10 and 13, and 1 or 2days after ovulation) to measure follicular and corpus luteum development. Hormone levels were measured using the corresponding ARCHITECT assay and were synchronized to the LH peak. Median, and 5th and 95th percentile values were determined for each day of the cycle, as well as for early follicular (days −15 to −6), late follicular (days −5 to −1), LH peak (day 0), early luteal (+1 to +4), mid-luteal (days +5 to +9), and late luteal (days +10 to +14) phases of the cycle. Based on our data, we were able to establish detailed reference values for LH, FSH, estradiol, and progesterone, which should aid in the interpretation of results for these reproductive hormones in a variety of circumstances.

Clin Chem Lab Med 2006;44:883–7.

  • 1.

    Gronowski AN, Landau-Levine ME. Reproductive endocrine function. In: Burtis CA, Ashwood ER, editors. Tietz fundamentals of clinical chemistry. Philadelphia: WB Saunders, 2001:877–97.

  • 2.

    Marshall JC. Hormonal regulation of the menstrual cycle and mechanisms of ovulation. In: DeGroot LJ, Jameson JL, editors. Endocrinology. Philadelphia, PA: WB Saunders, 2001:2073–85.

  • 3.

    Clinical and Laboratory Standards Institute. How to define and determine reference intervals in the clinical laboratory; approved guideline, 2nd ed. Document c28-A2. Villanova, PA: CLSI, 2000.

  • 4.

    Dighe AS, Moy JM, Hayes FJ, Sluss PM. High-resolution reference ranges for estradiol, luteinizing hormone, and follicle stimulating hormone in men and women using the AxSYM assay system. Clin Biochem 2005; 38:175–9.

  • 5.

    Quinn FA. ARCHITECT i2000 and i2000SR analyzers. In: Wild D, editor. The immunoassay handbook, 3rd ed. Amsterdam: Elsevier, 2005:406–11.

  • 6.

    Yang DT, Owen WE, Ramsay CS, Xie H, Roberts WL. Performance characteristics of eight estradiol immunoassays. Am J Clin Pathol 2004; 122:332–7.

  • 7.

    Hendriks HA, Kortlandt W, Verweij WM. Analytical performance comparison of five new generation immunoassay analyzers. Ned Tijdschr Klin Chem 2000;25:170–7.

  • 8.

    Verheecke P, Quinn FA. Laboratory evaluation of representative disease state assays on the Abbott ARCHITECT® i2000® analyzer. J Assoc Lab Automat 2000; 5:30–3.

  • 9.

    Rufo G, Brookhart P, Barnes W, Frels M, Sheu M, White M, et al. Performance characteristics of the Abbott ARCHITECT progesterone assay [abstract]. Clin Chem 2005; 51(6 Suppl):A235.

  • 10.

    Stanton PG, Burgon PG, Hearn MT, Robertson DM. Structural and functional characterization of hFSH and hLH isoforms. Mol Cell Endocrinol 1996; 125:133–41.

  • 11.

    Anobile CJ, Talbot JA, McCann SJ, Padmanabhan V, Robertson WR. Glycoform composition of serum gonadotropins through the normal menstrual cycle and in the post-menopause state. Mol Hum Reprod 1998; 47:631–9.

  • 12.

    Taieb J, Olivennes F, Birr AS, Benattarc C, Righini C, Frydman R, et al. Comparison of day 3 FSH values as determined by six different immunoassays. Hum Reprod 2002; 17:926–8.

Purchase article
Get instant unlimited access to the article.
$42.00
Log in
Already have access? Please log in.


or
Log in with your institution

Journal + Issues

Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

Search