Apolipoprotein E polymorphism – a risk factor for metabolic syndrome

Anca Sima 1 , Alexandru Iordan 2 ,  and Camelia Stancu 3
  • 1 Department of Lipoproteins and Atherosclerosis, Institute of Cellular Biology and Pathology “Nicolae Simionescu”, Bucharest, Romania
  • 2 Department of Lipoproteins and Atherosclerosis, Institute of Cellular Biology and Pathology “Nicolae Simionescu”, Bucharest, Romania
  • 3 Department of Lipoproteins and Atherosclerosis, Institute of Cellular Biology and Pathology “Nicolae Simionescu”, Bucharest, Romania

Abstract

Background: Metabolic syndrome is closely related to several disturbances in lipid and lipoprotein metabolism. The aim of this study was to determine the association between apolipoprotein E (apoE) genotypes and the risk of metabolic syndrome and/or coronary heart disease complications.

Methods: The study included 279 subjects divided into three groups: 1) control subjects, 2) metabolic syndrome patients, and 3) obese patients with coronary heart disease. All subjects were characterized by body mass index, and plasma levels of glucose, triglycerides, cholesterol, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). ApoE genotypes were identified by PCR-restriction fragment length polymorphism using genomic DNA.

Results: Statistical analysis of plasma parameters showed that subjects in groups 2 and 3 had higher levels of triglycerides and lower levels of HDL-C compared to group 1. The frequencies of apoE genotypes determined in this Romanian population (65% for E3/3, 19.6% for E4/3, 9.5% for E3/2, 4.1% for E2/2, 0.6% for E4/4, 1.3% for E4/2) were in agreement with those reported for other Caucasian populations. The distribution of apoE alleles indicated a higher frequency of ɛ4 in groups 2 and 3. There was a higher frequency of the apoE4/3 genotype in groups 2 and 3, which was significantly correlated with higher levels of triglycerides and lower levels of HDL-C.

Conclusions: Correlations of apoE genotypes with these markers indicate that the ɛ4 allele is an independent risk factor for metabolic syndrome.

Clin Chem Lab Med 2007;45:1149–53.

Purchase article
Get instant unlimited access to the article.
$42.00
Log in
Already have access? Please log in.


or
Log in with your institution

Journal + Issues

Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

Search