Breath isoprene – aspects of normal physiology related to age, gender and cholesterol profile as determined in a proton transfer reaction mass spectrometry study

Ievgeniia Kushch 1 , 1 , Barbora Arendacká 2 , 2 , Svorad Štolc 3 , 3 , Pawel Mochalski 4 , 4 , Wojciech Filipiak 5 , 5 , Konrad Schwarz 6 , 6 , Lukas Schwentner 7 , 7 , Alex Schmid 8 , 8 , Alexander Dzien 9 , 9 , Monika Lechleitner 10 , 10 , Viktor Witkovský 11 , 11 , Wolfram Miekisch 12 , 12 , Jochen Schubert 13 , 13 , Karl Unterkofler 14 , 14  and Anton Amann 15 , 15
  • 1 Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria and Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria
  • 2 Institute of Measurement Science, Slovak Academy of Sciences, Bratislava, Slovak Republic
  • 3 Institute of Measurement Science, Slovak Academy of Sciences, Bratislava, Slovak Republic
  • 4 Institute of Nuclear Physics PAN, Krakow, Poland
  • 5 Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria and Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria
  • 6 Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria and Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria
  • 7 Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria and Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria
  • 8 Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria and Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria
  • 9 Innsbruck, Austria
  • 10 Landeskrankenhaus Hochzirl, Anna-Dengel-Haus, Hochzirl, Austria
  • 11 Institute of Measurement Science, Slovak Academy of Sciences, Bratislava, Slovak Republic
  • 12 Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria and University of Rostock, Department of Anaesthesiology and Intensive Care, Rostock, Germany
  • 13 Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria and University of Rostock, Department of Anaesthesiology and Intensive Care, Rostock, Germany
  • 14 Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria and University of Applied Sciences, Dornbirn, Austria
  • 15 Department of Anaesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria and Breath Research Unit, Austrian Academy of Sciences, Dornbirn, Austria

Abstract

Background: This study was performed to clarify variations in breath isoprene concentrations with age, gender, body mass index (BMI) and total serum cholesterol. Our cohort consisted of 205 adult volunteers of different smoking background without health complaints. Total cholesterol in blood serum was measured in 79 of these volunteers.

Methods: Mixed expiratory exhaled breath was sampled using Tedlar bags. Concentrations of isoprene were then determined using proton transfer reaction-mass spectrometry.

Results: Isoprene concentrations ranged from 5.8 to 274.9 ppb, with an overall geometric mean (GM) of 99.3 ppb. There was no statistically significant difference in mean isoprene in breath between males and females (GM 105.4 and 95.5 ppb, respectively). Ageing led to a decrease in concentration in men, with an estimated slope of the regression line for log-transformed isoprene concentrations of –0.0049, but did not influence isoprene levels in women. We did not observe any significant correlation between isoprene breath content and cholesterol level in blood, even after adjusting for the possible influence of age. Similarly, no correlation was found between isoprene levels and BMI.

Conclusions: Isoprene concentrations in exhaled breath showed gender-specific correlations with respect to age. Further investigations are necessary to clarify the relation between isoprene concentrations in exhaled breath and cholesterol levels and synthesis rates in blood.

Clin Chem Lab Med 2008;46:1011–8.

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Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

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