Performance evaluation of the digital cell imaging analyzer DI-60 integrated into the fully automated Sysmex XN hematology analyzer system

Yoko Tabe 1 , Takemasa Yamamoto 2 , Imiko Maenou 2 , Rie Nakai 3 , Mayumi Idei 1 , Takashi Horii 2 , Takashi Miida 1 ,  and Akimichi Ohsaka 2
  • 1 Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan
  • 2 Division of Clinical Laboratory, Juntendo University Hospital, Bunkyo-ku, Tokyo, Japan
  • 3 Scientific Affairs, Sysmex Corporation, Kobe, Hyogo, Japan
Yoko Tabe, Takemasa Yamamoto, Imiko Maenou, Rie Nakai, Mayumi Idei, Takashi Horii, Takashi Miida and Akimichi Ohsaka

Abstract

Background: The XN-Series (Sysmex, Kobe, Japan) have been equipped with the automated digital cell imaging analyzer DI-60, which provides complete automation of the sample processing with automated complete blood counts (CBC), slide making/staining, and digital scanning with cell pre-classification. The aim of this study was to evaluate the efficacy of the XN-Series as an integrated blood cell analysis system.

Methods: White blood cell (WBC) morphological analysis by the DI-60 was evaluated using 232 blood samples from patients. Routine analysis of a total of 2000 blood samples has been performed to evaluate the processing ability of the XN-Series connected to the DI-60.

Results: The overall analysis accuracy of pre-classification of WBC by the DI-60 was 88.4%. Good correlation was observed between final results of the DI-60 analysis and manual differentiation with high sensitivity and specificity for blasts and immature granulocytes. The sample processing time of the XN-Series, from automated CBC to cell pre-classification, was 38±1 min/single run and 165±12 min/500 CBC samples run (slide preparation rate 15.6%) with no sample hold-up at the DI-60.

Conclusions: The automated morphological analysis capability of the DI-60 has potential usefulness in the integrated automated hematology analysis system of XN-Series.

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Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

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