Measurement of free light chains with assays based on monoclonal antibodies

Henk te Velthuis 1 , Mark Drayson 2  and John P. Campbell 2
  • 1 Sanquin Reagents and Sanquin Research, Sanquin Blood Supply Foundation and Landsteiner Laboratory, Academic Medical Center, Amsterdam, The Netherlands
  • 2 Institute of Immunology and Immunotherapy
Henk te Velthuis, Mark Drayson and John P. Campbell

Abstract

Recently, serum free light chain (FLC) assays incorporating anti-kappa (κ) and anti-lambda (λ) FLC monoclonal antibodies have become available: N Latex FLC assay (Siemens) and Seralite® (Abingdon Health). The purpose of this review is to provide an overview of these two new monoclonal antibody-based methods. In doing so, the review will outline the performance characteristics of each method, including a summary of: assay principles, antibody specificity, analytical performance and assay performance in disease. Additionally, the review will describe the potential user benefits of adopting these new generation FLC assays, which are designed to overcome the established limitations of existing polyclonal antibody based FLC assays.

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Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

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