The effect of storage temperature fluctuations on the stability of biochemical analytes in blood serum

Helmuth Haslacherhttp://orcid.org/0000-0003-4605-2503 1 , Thomas Szekeres 1 , Marlene Gerner 1 , Elisabeth Ponweiser 1 , Manuela Repl 1 , Oswald F. Wagner 1  and Thomas Perkmann 2
  • 1 Department of Laboratory Medicine, Vienna, Austria
  • 2 Department of Laboratory Medicine, Waehringer Guertel 18-20, 1090 Vienna, Austria
Helmuth HaslacherORCID iD: http://orcid.org/0000-0003-4605-2503, Thomas Szekeres, Marlene Gerner, Elisabeth Ponweiser, Manuela Repl, Oswald F. Wagner and Thomas Perkmann
  • Corresponding author
  • Department of Laboratory Medicine, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria
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Abstract

Background:

Irreproducibility of scientific results constitutes an undesirably onerous economic burden and is in many cases caused by low-quality materials. Therefore, researchers are increasingly devoting their attention to the bioresources they use. In turn, those bioresources are required to validate their preanalytical processes in order to ensure best possible quality. The present study thus aimed to evaluate the impact of repeated temperature fluctuations, as they occur in most research biobanks due to repetitive opening and closing of freezer doors, on the stability of 26 biochemical analytes.

Methods:

Serum of 43 individuals was randomly assigned to a fluctuation (n=21) and a control group (n=22). Serum of the fluctuation group underwent controlled temperature fluctuations (30 fluctuations <−75°C – <−65°C – <−75°C under real-life freezer conditions within 21 days). Control sera were stored at constant conditions. After 10, 20, and 30 fluctuations, results derived from the fluctuation group were compared to baseline and to the control group by means of general linear models.

Results:

Sixteen biomarkers showed statistically significant changes over time, whereas only seven of those presented with diagnostically/clinically relevant changes at certain time points (aspartate aminotransferase, amylase, calcium, uric acid, creatinine, inorganic phosphate and total protein). However, there was no difference between the fluctuation and the control group.

Conclusions:

Some serum analytes are influenced by storage, even at temperatures as low as <−70°C. In contrast, we found no evidence that complex temperature fluctuations produced by storage of and access to biospecimens in biobank freezers generate any additional variability.

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Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

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