Neutralization of biotin interference: preliminary evaluation of the VeraTest Biotin™, VeraPrep Biotin™ and BioT-Filter®

Julien Favresse
  • Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Université catholique de Louvain, Brussels, Belgium
  • Department of Laboratory Medicine, Clinique St-Luc Bouge, Namur, Belgium
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, Jean-Louis Bayart
  • Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Université catholique de Louvain, Brussels, Belgium
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, Anke Stoefs
  • Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Université catholique de Louvain, Brussels, Belgium
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, Damien Gheldof
  • Department of Pharmacy, Namur Research Institute for Life Sciences, Namur Thrombosis and Hemostasis Center, University of Namur, Namur, Belgium
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, Jonathan DouxfilsORCID iD: https://orcid.org/0000-0002-7644-5298, Jean-Michel Dogné
  • Department of Pharmacy, Namur Research Institute for Life Sciences, Namur Thrombosis and Hemostasis Center, University of Namur, Namur, Belgium
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and Damien Gruson
  • Corresponding author
  • Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Université catholique de Louvain, Brussels, Belgium
  • Pôle de recherche en Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Cliniques Universitaires St-Luc and Université Catholique de Louvain, Tour Claude Bernard, Brussels, Belgium
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    Favresse J, Burlacu MC, Maiter D, Gruson D. Interferences with thyroid function immunoassays: clinical implications and detection algorithm. Endocr Rev 2018;39:830–50.

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    Bayart JL, Favresse J, Stoefs A, Closset M, Roy T, Fillee C, et al. Biotin interferences: have we neglected the impact on serological markers? Clin Chim Acta 2020;503:107–12.

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    Piketty ML, Prie D, Sedel F, Bernard D, Hercend C, Chanson P, et al. High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference. Clin Chem Lab Med 2017;55: 817–25.

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    https://www.veravas.com/products/veraprep-biotin. Accessed on 12 December 2019.

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    Bouvy C, Evrard J, Siriez R, Mullier F, Douxfils J. Removal of DOACs from plasma: performance comparison and pre-analytical considerations of three different devices. Poster session presented at European Congress on Thrombosis and Haemostasis, Marseille, France, 2018.

  • 9.

    IDK Biotin ELISA package insert reference, Immundiagnostik AG, Stubenwald-Alleeb 8a, 64625 Bensheim, Germany.

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    Fujiwara M, Ando I, Yagi S, Nishizawa M, Oguma S, Satoh K, et al. Plasma levels of biotin metabolites are elevated in hemodialysis patients with cramps. Tohoku J Exp Med 2016;239:263–7.

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    Yagi S, Nishizawa M, Ando I, Oguma S, Sato E, Imai Y, et al. A simple and rapid ultra-high-performance liquid chromatography-tandem mass spectrometry method to determine plasma biotin in hemodialysis patients. Biomed Chromatogr 2016;30:1285–90.

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    https://www.fda.gov/medical-devices/safety-communications/update-fda-warns-biotin-may-interfere-lab-tests-fda-safety-communication. Accessed on 12 December 2019.

  • 13.

    Favresse J, Gruson D, Gheldof D. Comment on “High doses of biotin can interfere with immunoassays that use biotin-strept(avidin) technologies: implications for individuals with biotin-responsive inherited metabolic disorders”. Mol Genet Metab Rep 2019;21:100506.

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Clinical Chemistry and Laboratory Medicine ( CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor of over three. CCLM is the official journal of nine national clinical societies and associated with EFLM.

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