The purpose of this study is to develop a method to discriminate spectral signatures in wound tissue. We have collected a training set of the intensity of the remitted light for different types of wound tissue from different patients using a TIVITA™ tissue camera. We used a neural network technique (self-organizing map) to group areas with the same spectral properties together. The results of this work indicates that neural network models are capable of finding clusters of closely related hyperspectral signatures in wound tissue, and thus can be used as a powerful tool to reach the anticipated classification. Moreover, we used a least square method to fit literature spectra (i.e. oxygenated haemoglobin (O2Hb), deoxygenated haemoglobin (HHb), water and fat) to the learned spectral classes. This procedure enables us to label each spectral class with the corresponding absorbance properties for the different absorbance of interest (i.e. O2Hb, HHb, water and fat). The calculated parameters of a testing set were consistent with the expected behaviour and show a good agreement with the results of a second algorithm which is used in the TIVITA™ tissue camera.
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