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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access December 1, 2006

Sequencing peptides by electrospray ion-trap mass spectrometry: A useful tool in synthesis of Axinastatin 3

  • Chenxi Jia EMAIL logo , Wei Qi , Zhimin He and Bin Qiao
From the journal Open Chemistry

Abstract

Axinastatin 3 as a potential anticancer agent was synthesized by chemical methods. In an electrospray ion-trap mass spectrometer, using one stage of tandem mass spectrometry (MS/MS), the linear peptide intermediate was sequenced via the complementarities of y and b ions. Then, using multistep MS/MS (to MS6), the cyclic peptide was sequenced through sequentially removing one amino acid residue in each stage of MS/MS. The difference of the fragmentation mechanisms and the sequencing approaches between them is discussed.

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Published Online: 2006-12-1
Published in Print: 2006-12-1

© 2006 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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