Screen-printed electrodes modified with HRP-zirconium alcoxide film for the development of a biosensor for acetaminophen detection

Veronica Sima 1 , Cecilia Cristea 1 , Ede Bodoki 1 , Gabriela Duţu 1  and Robert Sāndulescu 1
  • 1 Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania

Abstract

The development of a biosensor based on the immobilization of horseradish peroxidase (HRP) within a zirconium alkoxide-polyetilenimine film onto screen-printed electrodes (SPE) for acetaminophen detection and acetaminophen quantification in pharmaceutical products is described. The biosensor operation mode is based on monitoring the amperometric signal produced by the electrochemical reduction of the enzymatically generated electroactive oxidized species of acetaminophen in the presence of hydrogen peroxide. The enzyme immobilization is performed by retention in a polyethylenimine-zirconium alcoxide porous gel film, a technique that offers good entrapping and a protective environment for the biocomponent due to the hydration properties of the immobilization layer. SPEs have the advantage of being easily mass-produced at low costs with superior characteristics in comparison with classical electrode materials. In this configuration, zirconium alkoxide demonstrates its electrocatalytic activity. The biosensor allows the quantification of acetaminophen with a limit of detection of 6.21×10−8 M and a linear range between 4.35×10−7 M and 4.98×10−6 M. Finally, the biosensor is applied to the quantitative analysis of acetaminophen in Perdolan® tablets.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1] S.V. Dzyadevych, V.N. Arkhypova, A.P. Soldatkin, A.V. Elskaya, C. Martelet, N. Jaffrezic-Renault, ITBM-RBM 29, 171 (2008)

  • [2] D.R. Thevenot, K. Toth, R.A. Durst et al., Biosens. Bioelectron. 16, 121 (2001) http://dx.doi.org/10.1016/S0956-5663(01)00115-4

  • [3] J. Wang, Analyst 119, 763 (1994) http://dx.doi.org/10.1039/an9941900763

  • [4] X. Xu, S. Zhang, H. Chen, J. Kong, Talanta 80,1, 8 (2009) http://dx.doi.org/10.1016/j.talanta.2009.06.039

  • [5] I. Palchetti, M. Mascini, M. Minunni, A.R. Bilia, F.F. Vincieri, J. Pharm. Biomed. Anal. 32, 2, 251 (2003) http://dx.doi.org/10.1016/S0731-7085(03)00132-8

  • [6] M.L. Rodriguez-Mendez, M. Gay, C. Apetrei, J.A. De Saja, Electrochim. acta 54, 27, 7033 (2009) http://dx.doi.org/10.1016/j.electacta.2009.07.024

  • [7] Y. Zhang, P. He, N. Hu, Electrochem. Acta 49, 1981 (2004) http://dx.doi.org/10.1016/j.electacta.2003.12.028

  • [8] T.J. Castilho, M.P. Taboada Sotomayor, L.T. Kubota, J. Pharm. Biomed. Anal. 37, 785 (2005) http://dx.doi.org/10.1016/j.jpba.2004.11.043

  • [9] J. Wu, F. Yan, X. Zhang, Y. Yan, J. Tang, H. Ju, Clin. Chem. 54, 1481 (2008) http://dx.doi.org/10.1373/clinchem.2007.102350

  • [10] N.V. Panini, G.A. Messina, E. Salinas, H. Fernandez, J. Raba, Biosens. Bioelectron. 23, 1145 (2008) http://dx.doi.org/10.1016/j.bios.2007.11.003

  • [11] X.H. Fu, Bioprocess. Biosyst. Eng. 31, 69 (2008) http://dx.doi.org/10.1007/s00449-007-0147-7

  • [12] R.I. Stefan, R.G. Bokretsion, J.F. Staden, H.Y. Aboul-Enein, J. Pharm. Biomed. Anal. 33, 323 (2003) http://dx.doi.org/10.1016/S0731-7085(03)00284-X

  • [13] V. Hooda, A. Gahlaut, H. Kumar, C.S. Pundir, Sens. Actuators B 136, 235 (2009) http://dx.doi.org/10.1016/j.snb.2008.09.047

  • [14] J. Poljakova, K. Forsterova, M. Sulc, E. Frei, M. Stiborova, Biomed. Pap. Med. Fac. Univ. Palacky, Olomouc Czech Rep. 149, 449 (2005) (In Czech)

  • [15] J. Lin, C. He, Y. Zhao, S. Zhang, Sens. Actuators B 137, 768 (2009) http://dx.doi.org/10.1016/j.snb.2009.01.033

  • [16] F. Ghamouss, S. Ledru, N. Ruille, F. Lantier, M. Boujtita, Anal. Chim. Acta 570, 158 (2006) http://dx.doi.org/10.1016/j.aca.2006.04.022

  • [17] D. Yu, B. Blankert, J.M. Kauffmann, Biosens. Bioelectron. 22, 2707 (2007) http://dx.doi.org/10.1016/j.bios.2006.11.013

  • [18] A. Elyacoubi, S.I. Zayed, B. Blankert, J.M. Kauffmann, Electroanalysis 18, 4, 345 (2006) http://dx.doi.org/10.1002/elan.200503418

  • [19] Z. Tong, R. Yuan, Y. Chai, Y. Xie, S. Chen, J. Biotechnol. 128, 567 (2007) http://dx.doi.org/10.1016/j.jbiotec.2006.12.008

  • [20] S. Yang, Z. Chen, X. Jin, X. Lin, Electrochim. Acta 52, 200 (2006) http://dx.doi.org/10.1016/j.electacta.2006.04.059

  • [21] A.M. Azevedo, D.M. Prazeres, J.M. Cabral, L.P. Fonseca, Biosens. Bioelectron. 21, 235 (2005) http://dx.doi.org/10.1016/j.bios.2004.09.030

  • [22] S. Liu, Z. Dai, H. Chen, H. Ju, Biosens. Bioelectron. 9, 963 (2004) http://dx.doi.org/10.1016/j.bios.2003.08.025

  • [23] Y. Zhang, X. Chen, W. Yang, Sensors Actuators B 130, 682 (2008) http://dx.doi.org/10.1016/j.snb.2007.10.034

  • [24] Y. Yang, Z. Wang, M. Yang, J. Li, F. Zheng, G. Shen, R. Yu, Anal. Chim. Acta 584, 268 (2007) http://dx.doi.org/10.1016/j.aca.2006.11.055

  • [25] X. Yang, X. Chen, L. Yang, W. Yang, Bioelectrochemistry 74, 90 (2008) http://dx.doi.org/10.1016/j.bioelechem.2008.05.002

  • [26] V. Sima, C. Cristea, F. Lăpăduş, I.O. Marian, A. Marian, R. Săndulescu, J. Pharm. Biomed. Anal. 48, 1195 (2008) http://dx.doi.org/10.1016/j.jpba.2008.08.018

  • [27] D. Yu, O. Dominguez Renedo, B. Blankert, V. Sima, R. Săndulescu, J. Arcos, J.M. Kauffmann, Electroanalysis 18, 17, 1637 (2006) http://dx.doi.org/10.1002/elan.200603579

  • [28] B. Liu, Y. Cao, D. Chon, J. Kong, J. Deng, Anal. Chim. Acta 478, 59 (2003) http://dx.doi.org/10.1016/S0003-2670(02)01480-0

  • [29] G.A. Messina, I.E. De Vito, J. Raba, Anal. Chim. Acta 559, 152 (2006) http://dx.doi.org/10.1016/j.aca.2005.11.072

OPEN ACCESS

Journal + Issues

Open Chemistry is a peer-reviewed, open access journal that publishes original research, reviews and short communications in the fields of chemistry in an ongoing way. Our central goal is to provide a hub for researchers working across all subjects to present their discoveries, and to be a forum for the discussion of the important issues in the field.

Search