Properties of fructosyltransferase from Aureobasidium pullulans immobilized on an acrylic carrier

Z. Onderková 1 , J. Bryjak 2  and M. Polakovič 1
  • 1 Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovak Republic
  • 2 Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Norwida 4/6, 50-373, Wroclaw, Poland

Abstract

Optimization of immobilization conditions was carried out for covalent binding of Aureobasidium pullulans fructosyltransferase to a copolymer of butyl acrylate and ethylene glycol dimethacrylate using a glutaraldehyde method. It was found that the highest activity of the preparation could be obtained for the immobilization pH 6.0 and initial protein amount 8.5 g per dm3 of the carrier. Effects of the reaction pH, temperature, and initial sucrose concentration on the activity and stability of the preparation were analyzed. Further investigations involved storage stability and operational stability in a mechanically stirred-tank reactor.

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