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Licensed Unlicensed Requires Authentication Published by De Gruyter October 1, 2007

Properties of fructosyltransferase from Aureobasidium pullulans immobilized on an acrylic carrier

  • Z. Onderková EMAIL logo , J. Bryjak and M. Polakovič
From the journal Chemical Papers

Abstract

Optimization of immobilization conditions was carried out for covalent binding of Aureobasidium pullulans fructosyltransferase to a copolymer of butyl acrylate and ethylene glycol dimethacrylate using a glutaraldehyde method. It was found that the highest activity of the preparation could be obtained for the immobilization pH 6.0 and initial protein amount 8.5 g per dm3 of the carrier. Effects of the reaction pH, temperature, and initial sucrose concentration on the activity and stability of the preparation were analyzed. Further investigations involved storage stability and operational stability in a mechanically stirred-tank reactor.

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Published Online: 2007-10-1
Published in Print: 2007-10-1

© 2007 Institute of Chemistry, Slovak Academy of Sciences

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