Synthesis and comparative characteristics of biological activities of (La, Sr)MnO3 and Fe3O4 nanoparticles

Olga Shydlovska 1 , Nadiya Zholobak 1 , Svitlana Dybkova 2 , Sergej Osinsky 3 , Larissa Bubnovskaya 3 , Oleksandr Yelenich 4 , Sergii Solopan 5 ,  and Anatolii Belous 5
  • 1 D.K. Zabolotny Institute of Microbiology and Virology, NAS of UKraine, Kiev, Ukraine
  • 2 F.D. Ovcharenko Institute of Biocolloidal Chemistry, NAS of UKraine, Kiev, Ukraine
  • 3 R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of UKraine, Kiev, Ukraine
  • 4 V.I. Vernadsky Institute of General and Inorganic Chemistry, National Academy of Sciences (NAS) of UKraine, Palladina Ave. 32/34, 03680, Kiev, Ukraine
  • 5 V.I. Vernadsky Institute of General and Inorganic Chemistry, NAS of UKraine, Kiev, Ukraine
Olga Shydlovska
  • Ukraine
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  • Olga Shydlovska is a PhD student at the National Academy of Sciencess (NAS) of Ukraine. Her areas of research include: biological properties, in particular, antiviral and antioxidant properties nanoparticles of different metals or their oxides, such as gold nanoparticles, iron oxide and cerium dioxide nanoparticles.
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, Nadiya Zholobak
  • Ukraine
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  • Nadiya Zholobak is a senior researcher with the Candidates of Sciences (PhD Biology). Her areas of research include: nanobiotechnology and antiviral substances search, studying of their effects in vitro and in vivo and nanotherapy of viral infections. She has published more than 100 scientific papers and has 26 patents in the Ukraine.
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, Svitlana Dybkova
  • Ukraine
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  • Svitlana Dybkova is a senior researcher with the Candidates of Sciences (PhD Biology). Her areas of research include: nanobiotechnology, nanomedicine, nanotoxicology and mechanisms of metal nanoparticles influence on prokaryotic cells, eukaryotic in vitro (normal and cancer) cells and in vivo. She has published 120 scientific papers and has eight patents in the Ukraine.
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, Sergej Osinsky
  • Ukraine
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  • Sergej Osinsky is a DMedSc. His areas of research include: tumor pathophysiology, in particular metabolic microenvironment (tumor pH, oxygenation, perfusion, energy status; modifying agents of cancer therapy, hypoxic radiosensitizers; hypoxia-associated molecular profile of tumors, in particular human gastric and pancreatic cancers, and its effect on malignant progression, its correlation with minimal residual disease. His other scientific interests include methods of nanohyperthermia of malignant tumors using ferromagnetic materials as inductors and creation of pH- and thermosensitive magnetic nanocomposites with immobilized cytostatics and/or proteins for selective antitumor therapy.
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, Larissa Bubnovskaya
  • Ukraine
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  • Larissa Bubnovskaya is a senior researcher with the candidates of Sciences (PhD, Biology). Her areas of research include: biochemical basis of the tumor pathophysiology, in particular tumor pH, oxygenation, perfusion, energy status (study with microelectrode technique, NMR spectroscopy, biochemical methods), and working out the anticancer as well as modifying agents of cancer therapy (local microwave hyperthermia induced hyperglycemia and hypoxic radiosensitizers), hypoxia-associated molecular and phospholipid profiles of human gastric cancer. Her other areas of research include the investigation of the biological activity of ferromagnetic materials in particular MFs in experiments in vivo.
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, Oleksandr Yelenich
  • Corresponding author
  • Ukraine
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  • Oleksandr Yelenich is a researcher with the Candidates of Sciences (PhD, Chemistry) at the V.I. Vernadsky Institute of General and Inorganic Chemistry, NAS of Ukraine, Kiyv, Ukraine. His areas of research include: studying the peculiarities of the synthesis and properties of ferrimagnetic nanoparticles with spinel structure and the synthesis and studying of functionalized magnetic homo- and heterostructures based on ferrite nanoparticles for advanced technical and biomedical applications.
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, Sergii Solopan
  • Ukraine
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  • Sergii Solopan is a senior researcher with the Candidate of Sciences (PhD, Chemistry). His areas of research included: nanosized Pb- and Sn-containing compounds with perovskite structure, synthesis and studying of ferromagnetic nanoparticles with spinel and perovskite structure.
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and Anatolii Belous
  • Ukraine
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  • Anatolii Belous is a Professor, and Head of the Department of Solid State Chemistry. He graduated from the Kiev Polytechnic Institute, in the Radio Electronic Faculty in 1974. After that, he was a graduate student in the Karpov Physico-Chemistry Research Institute, Moscow and at the V.I. Vernadsky Institute of General and Inorganic Chemistry, NAS of Ukraine, Kiev, Ukraine. His areas of research include: the development of lithium-conducting solid electrolytes, microwave dielectrics, ferroelectrics type semiconductor and nanoscale ferromagnetic systems.
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Abstract

In the last decade, ferromagnetic nanoparticles that are able to be heated under an AMF (alternating magnetic field) have gained considerable interest in the field of nanotechnology. The current study explores the peculiarity of the synthesis and the properties of Fe3O4 and (La, Sr)MnO3 nanoparticles by cryochemical and sol-gel technology, as well the comparative analysis of biological activities of synthesized nanoparticles on different cell lines: the ST cell line (diploid epithelial swine testicular cell line) and the MCF-7S cell line (human breast cancer line). In the study, Fe3O4 and (La, Sr)MnO3 nanoparticles with superparamagnetic properties were synthesized, and magnetic fluids based on them that were efficiently heated when subjected to an AMF (specific loss power ~33–37 W/g) were prepared. It was observed that the temperature of magnetic fluids based on Fe3O4 nanoparticles increases linearly to the time of the AMF exposition, whereas for (La, Sr)MnO3-based fluid, it stabilizes within a given temperature range. It was shown that the nanoparticles of (La, Sr)MnO3, unlike Fe3O4, are characterized by low toxicity, antioxidant activity and the ability to influence on cell-virus interaction on normal cell lines (ST cell line). The possibility of the magnetic fluids obtained in this work to generate heat under the AMF exposition and the lack of side effects make them a potential means for magnetic hyperthermia.

  • 1.

    Menter DG, Patterson SL, Logsdon CD, Kopetz S, Sood AK, Hawk ET. Convergence of nanotechnology and cancer prevention: are we there yet? Cancer Prev Res 2014;7:973–92.

    • Crossref
    • Export Citation
  • 2.

    Abdeen S, Praseetha PK. Diagnostics and treatment of metastatic cancers with magnetic nanoparticles. J Nanomed Biotherapeutic Discov 2013;3:115.

  • 3.

    Panariti AP, Miserocchi G, Rivolta I. The effect of nanoparticle on cellular behavior: disrupting or enabling function? Nanotechnol Sci App 2012;5:87–100.

  • 4.

    Wu KC-W, Yamauchi Y. Controlling physical features of mesoporous silica nanoparticles (MSNs) for emerging applications. J Mater Chem 2012;22:1251–6.

    • Crossref
    • Export Citation
  • 5.

    Lian HY, Hu M, Liu CH, Yamauchi Y, Wu KC. Highly biocompatible, hollow coordination polymer nanoparticles as cisplatin carriers for efficient intracellular drug delivery. Chem Commun 2012;48:5151–3.

    • Crossref
    • Export Citation
  • 6.

    Jadhav KS, Dumbare PS, Pande VV. Mesoporous silica nanoparticles (MSN): a nanonetwork and hierarchical structure in drug delivery. J Nanomed Res 2015;2:00043.

  • 7.

    Yang HW, Hua MY, Liu HL, Huang CY. Potential of magnetic nanoparticles for targeted drug delivery. Nanotechnol Sci Appl 2012;5:73–86.

    • PubMed
    • Export Citation
  • 8.

    Arruebo M, Valladares M, Gonzalez-Fernandez A. Antibody-conjugated nanoparticles for biomedical applications. J Nanomater 2009;2009:439389.

  • 9.

    Jaradat ZW. Nanotechnology and its role in advancing medicine. JSM Nanotechnol Nanomed 2013;1:1012.

  • 10.

    Pantic I. Magnetic nanoparticles in cancer diagnosis and treatment: novel approaches. Rev Adv Mater Sci 2010;26: 67–73.

  • 11.

    Yu M, Zhou C, Liu J, Hankins JD, Zheng J. Luminescent gold nanoparticles with pH dependent membrane adsorbtion. J Am Chem Soc 2011;133:11014–17.

    • Crossref
    • Export Citation
  • 12.

    Pankhurst QA, Thanh NT, Jones SK, Dobson J. Progress in application of magnetic nanoparties in biomedicine. J Phys D Appl Phys 2009;42:224001–5.

    • Crossref
    • Export Citation
  • 13.

    Jordan A, Wust P, Fähling H, John W, Hinz A, Felix R. Inductive heating of ferromagnetic particles and magnetic fluids: physical evaluation of their potential for hyperthermia. Int J Hyperthermia 1997;9:51–68.

  • 14.

    Neilsen OS, Horsman M, Overgaard J. A future hyperthermia in cancer treatment? E J Cancer 2001;37:1587–89.

    • Crossref
    • Export Citation
  • 15.

    Chan DC, Kirpotin D, Bunn PA. Synthesis and evaluation of colloidal magnetic iron-oxides for the site-specic radiofrequency induced hyperthermia of cancer. J Magn Magn Mater 1993;122:374–78.

    • Crossref
    • Export Citation
  • 16.

    Pankhurst QA, Connolly J, Jones SK, Dobson J. Applications of magnetic nanoparticles in biomedicine. J Phys D Appl Phys 2003;36:R167–81.

    • Crossref
    • Export Citation
  • 17.

    Lévy M, Wilhelm C, Siaugue JM, Horner O, Bacri JC, Gazeau F. Magnetically induced hyperthermia: size-dependent heating power of γ-Fe2O3 nanoparticles. J Phys Condens Matter 2008;20:204133.

    • Crossref
    • Export Citation
  • 18.

    Bellizzi G, Bucci OM. On the optimal choice of the exposure conditions and the nanoparticle feature in magnetic nanoparticle hyperthermia. Int J Hyperthermia 2010;26:389–403.

    • Crossref
    • Export Citation
  • 19.

    Lu AH, Salabas EL, Schuth F. Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Ed 2007;46:1222–44.

    • Crossref
    • Export Citation
  • 20.

    De la Presa P, Luengo Y, Multigner M, Costo R, Morales MP, Rivero G, et al. Study of heating efficiency as a function of concentration, size, and applied Field in γ-Fe2O3 nanoparticles. J Phys Chem C 2012;116:25602–10.

    • Crossref
    • Export Citation
  • 21.

    Sousa ME, Marcela B, Raap FV, Rivas PC, Zélis PM, Girardin P, et al. Stability and relaxation mechanisms of citric acid coated magnetite nanoparticles for magnetic hyperthermia. J Phys Chem C 2013;117:5436–45.

    • Crossref
    • Export Citation
  • 22.

    Jordan A, Scholz R, Maier-Hauff K, Johannsen M, Wust P, Nadobny J, et al. Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. J Magn Magn Mater 2001;225:118–26.

    • Crossref
    • Export Citation
  • 23.

    Johannsen M, Thiesen B, Wus P, Jordan A. Magnetic nanoparticle hyperthermia for prostate cancer. Int J Hyperthermia 2010;26:790–95.

    • Crossref
    • PubMed
    • Export Citation
  • 24.

    Pollert E, Kaman O, Veverka P, Veverka M, Maryško M, Závěta K, et al. Core-shell La1-xSrxMnO3 nanoparticles ascolloidal mediators for magnetic fluid hyperthermia. Phil Trans R Soc A 2010;268:4389–405.

  • 25.

    Bubnovskaya L, Belous A, Solopan S, Kovelskaya L, Bovkun A, Podoltsev I, et al. Magnetic fluid hyperthermia of rodent tumors using manganese perovskite nanoparticles. J Nanopart 2014;2014:278761.

  • 26.

    Donaldson K, Tran L, Jimenez LA, Duffin R, Newby DE, Mills N, et al. Combustion-derived nanoparticles: a review of their toxicology following inhalation exposure. Part Fibre Toxicol 2005;2:10.

    • Crossref
    • PubMed
    • Export Citation
  • 27.

    Kabanov AV. Polymer genomics: an insight into pharmacology and toxicology of nanomedicines. Adv Drug Deliv Rev 2006;58:1597–627.

    • Crossref
    • PubMed
    • Export Citation
  • 28.

    Uboldi C, Bonacchi D, Lorenzi G, Hermanns MI, Pohl C, Baldi G, et al. Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441. Part Fibre Toxicol 2009;6:18.

    • Crossref
    • PubMed
    • Export Citation
  • 29.

    Pham HL, Nguyen CT, Nguyen AT, Pham VT, Tran CY, Nguyen TQ, et al. Invitro toxicity test and searching the possibility of cancer cell line extermination by magnetic heating with using Fe3O4 magnetic fluid. J Phys Confer Ser 2009;187:012008.

    • Crossref
    • Export Citation
  • 30.

    Kiroshka VV, Repin NV, Nadutov VM, Perekos AE, Voynash VZ, Tischenko Y, et al. Synthesis, biological activity and cytotoxity of nanoparticles based on Fe3O4. Nanosys Nanomater Nanotechnol 2010;8:787–98. (in Russian).

  • 31.

    Töpfer J, Angermann A. Nanocrystalline magnetite and Mn–Zn ferrite particles via the polyol process: Synthesis and magnetic properties. Mater Chem Phys 2011;129:337–42.

    • Crossref
    • Export Citation
  • 32.

    Brusentsova TN, Brusentsov NA, Kuznetsov VD, Nikiforov VN. Synthesis and investigation of magnetic properties of Gd-substituted Mn-Zn ferrite nanoparticles as a potential low-TC agent for magnetic fluid hyperthermia. J Magn Magn Mater 2005;293:298–302.

    • Crossref
    • Export Citation
  • 33.

    Freese C, Uboldi C, Gibson MI, Unger RE, Weksler BB, Romero IA, et al. Uptake and cytotoxicity of citrate-coated gold nanospheres: comparative studies on human endothelial and epithelial cells. Part Fibre Toxicol 2012;9:23.

    • Crossref
    • PubMed
    • Export Citation
  • 34.

    Liu L, Ni F, Zhang J, Jiang X, Lu X, Guo Z, et al. Silver nanocrystals sensitize magnetic-nanoparticle-mediated thermo-induced killing of cancer cells. Acta Biochim Biophys Sin 2011;43: 316–23.

    • Crossref
    • Export Citation
  • 35.

    Kale SN, Arora S, Bhayani KR, Paknikar KM, Jani M, Wagh UV, et al. Cerium doping and stoichiometry control for biomedicaluse of La0.7Sr0.3MnO3 nanoparticles: microwave absorption and cytotoxicity study. Nanomedicine 2006;2:217–21.

    • Crossref
    • PubMed
    • Export Citation
  • 36.

    Yelenich OV, Solopan SO, Kolodiazhnyi TV, Greneche JM, Belous AG. Synthesis of iron oxide nanoparticles by different methods and study of their properties. Solid State Phenom 2015;230:108–13.

    • Crossref
    • Export Citation
  • 37.

    Solopan SO, V’yunov OI, Belous AG, Polek TI, Tovstolytkin AI. Effect of nanoparticles agglomeration on electrical properties of La1−xAxMnO3 (A=Sr, Ba) nanopowder and ceramic solid solutions. Solid State Sci 2012;14:501–5.

    • Crossref
    • Export Citation
  • 38.

    Solopan SA, Belous A, Yelenich O, Bubnovskaya L, Kovelskaya A, Podoltsev A, et al. Nanohyperthermia of malignant tumors. I. lanthanum-strontium manganite magnetic fluid as potential inducer of tumor hyperthermia. Exp Oncol 2011;33:130–5.

    • PubMed
    • Export Citation
  • 39.

    Pashley RM, Marilyn EK. Applied colloid and surface chemistry. 7th ed. Chichester, West Sussex: J. Wiley; 2004. Internet resource.

    • Crossref
    • Export Citation
  • 40.

    Peddis D, Orrù F, Ardu A, Cannas C, Musinu A, Piccaluga G. Interparticle interactions and magnetic anisotropy in cobalt ferrite nanoparticles: influence of molecular coating. Chem Mater 2012;24:1062–71.

    • Crossref
    • Export Citation
  • 41.

    Veverka M, Zaveta K, Kaman O, Veverka P, Knizek K, Pollert E, et al. Magnetic heating by silica-coated Co-Zn ferrite particles. J Phys D Appl Phys 2014;47:065503.

    • Crossref
    • Export Citation
  • 42.

    Ferrari M, Fornasiero MC, Isetta AM. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods 1990;131:165–72.

    • Crossref
    • PubMed
    • Export Citation
  • 43.

    Bogorad-Kobelska OS, Zholobak NM, Olevinska ZM, Spivak MY. The antiviral activity of diphenyl derivatives in different model systems. Fiziol Zh 2012;1:36–42. (in Ukrainian).

  • 44.

    Olive PL, Banáth JP. The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 2006;1:23–9.

    • Crossref
    • PubMed
    • Export Citation
  • 45.

    King AM, Adams MJ, Carstens EB, Lefkowitz EJ, editors. Virus Taxonomy. Classification and nomenclature of viruses. Ningh report of International Committee on the Taxonomy of viruses. London: Elsevier Academic Press; 2011. 1327 p.

  • 46.

    Medvedev AE, Fuchs BB, Rankhmilevich AL. A study of the action of immunosuppressive factors from tumour cells on lymphocytes and macrophages in vitro and on the graft-versus-host reaction in mice. Biomed Sci 1990;1:261–6.

    • PubMed
    • Export Citation
  • 47.

    Stefanov OV. Doklinichni doslidzhennya likarskykh zasobiv (metodychni rekomendatsii) [Preclinical studies of drugs (Guidelines)]. Kyiv: Avicena Publ., 2001. 528 p. (in Ukrainian).

  • 48.

    Zholobak NM, Shcherbakov AB, Bogorad-Kobelska AS, Ivanova OS, Baranchikov AY, Spivak NY, et al. Panthenol-stabilized cerium dioxide nanoparticles for cosmeceutic formulations against ROS-induced and UV-induced damage. J Photoch Photobio B 2014;130:102–8.

    • Crossref
    • Export Citation
  • 49.

    Cornell RM, Schwertmann U. The iron oxides: structure, properties, reactions, occurrences and uses. 2nd ed. Weinheim, New York: Wiley-VCH; 2003. 703 p.

    • Crossref
    • Export Citation
  • 50.

    Singh N, Jenkins GJ, Asadi R, Doak SH. Potential toxicity of supermagnetic iron nanoparticles (SPION). Nano Rev 2010;1:1–15.

  • 51.

    Schwarz KB. Oxidative stress during viral infection: a review. Free Radic Biol Med 1996;21:641–9.

    • Crossref
    • PubMed
    • Export Citation
  • 52.

    Reshi ML, Su YC, Hong JR. RNA viruses: ROS-mediated cell death. Int J Biochem Cell Biol 2014;2014:467452.

  • 53.

    Liou GY, Storz P. Reactive oxygen species in cancer. Free Radical Res 2010;44:479–96.

    • Crossref
    • Export Citation
  • 54.

    Valdiglesias V, Kilic G, Costa C, Fernández-Bertólez N, Pásaro E, Teixeira JP, et al. Effects of iron oxide nanoparticles: cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity. Environ Mol Mutagen 2014;56:125–48.

    • PubMed
    • Export Citation
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