Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood

Qushmua E. Alzahrani, Gary G. Adams, Richard B. Gillis 1 , Tabot M.D. Besong 1 , M. Samil Kök, Emily Fong 1 , Richard A. Harding 1 , Jan E.G. van Dam 4 , Richard J.A. Gosselinkhttp://orcid.org/0000-0001-7733-6109 4 , Arthur J. Rowe 1 ,  and Stephen E. Harding 1
  • 1 National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, College Road, Sutton, Bonington LE12 5RD, UK
  • 2 Faculty of Medicine and Health Science, University of Nottingham, Queens Medical Centre, Nottingham NG7 2RD, UK
  • 3 Department of Food Engineering, Abant Izzet Baysal University, 14280 Bolu, Turkey
  • 4 Food and Biobased Research, Wageningen UR, Bornse Weilanden 9, NL-6708 WG Wageningen, The Netherlands
Qushmua E. Alzahrani, Gary G. Adams, Richard B. Gillis, Tabot M.D. Besong, M. Samil Kök, Emily Fong, Richard A. Harding, Jan E.G. van Dam, Richard J.A. GosselinkORCID iD: http://orcid.org/0000-0001-7733-6109, Arthur J. Rowe and Stephen E. Harding

Abstract

Molecular weight (MW) and related conformational data of three commercially available technical lignins (Alcell L, kraft L, and soda L) have been studied by means of analytical ultracentrifugation, taking advantage of some recent developments in both sedimentation velocity and sedimentation equilibrium determinations. The lignins were dissolved in dimethyl sulphoxide (with ca. 90% solubility), and solutions were studied with regards to their oligomeric state, heterogeneity profiles (distribution of sedimentation coefficients), and molecular weight distributions (MWD). Alcell L and soda L have similar properties showing one major low MW component and two minor high MW components, whereas kraft L appears to be larger and more uniform, i.e., it shows a more monodisperse MWD. Weight average molecular weight (Mw) data from sedimentation equilibrium obtained by the new SEDFIT-MSTAR procedure in conjunction with MULTISIG analysis were found to be ~18 kDa (Alcell L), 25 kDa (kraft L), and 15 kDa (soda L). Further analysis of the data by means of the routines MULTISIG and M_INVEQ confirmed the presence of additional components in Alcell L and soda L, and the larger size and high degree of monodispersity of kraft L. The intrinsic viscosity data of the three lignins were found to be very similar in the range of 22–24 ml g-1, and all data were consistent with an elongated plate shape molecular structure with an equivalent discoid aspect ratio ~30.

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Holzforschung is an international scholarly journal that publishes cutting-edge research on the biology, chemistry, physics and technology of wood and wood components. High quality papers about biotechnology and tree genetics are also welcome. Rated year after year as one of the top scientific journals in the category of Pulp and Paper (ISI Journal Citation Index), Holzforschung represents innovative, high quality basic and applied research.

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