Bioconversion of pine stumps to ethanol: pretreatment and simultaneous saccharification and fermentation

Cátia Vanessa Teixeira Mendes 1 , Priscilla Vergara 2 , Jose María Carbajo 2 , Juan Carlos Villar 2 , Jorge Manuel dos Santos Rocha 1  and Maria da Graça Videira de Sousa Carvalho 1
  • 1 CIEPQPF, Department of Chemical Engineering, University of Coimbra, R. Sílvio Lima, Pólo II, 3030-790 Coimbra, Portugal
  • 2 INIA, National Institute of Agricultural and Food Research and Technology – Pulp and Paper Laboratories, Madrid, Spain
Cátia Vanessa Teixeira Mendes
  • CIEPQPF, Department of Chemical Engineering, University of Coimbra, R. Sílvio Lima, Pólo II, 3030-790 Coimbra, Portugal
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, Priscilla Vergara
  • INIA, National Institute of Agricultural and Food Research and Technology – Pulp and Paper Laboratories, Madrid, Spain
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, Jose María Carbajo
  • INIA, National Institute of Agricultural and Food Research and Technology – Pulp and Paper Laboratories, Madrid, Spain
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, Juan Carlos Villar
  • INIA, National Institute of Agricultural and Food Research and Technology – Pulp and Paper Laboratories, Madrid, Spain
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, Jorge Manuel dos Santos Rocha
  • CIEPQPF, Department of Chemical Engineering, University of Coimbra, R. Sílvio Lima, Pólo II, 3030-790 Coimbra, Portugal
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and Maria da Graça Videira de Sousa Carvalho
  • Corresponding author
  • CIEPQPF, Department of Chemical Engineering, University of Coimbra, R. Sílvio Lima, Pólo II, 3030-790 Coimbra, Portugal
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Abstract

The technical viability was investigated for the conversion of Pinus pinaster stumps to value-added products via a chain of pretreatments and simultaneous saccharification and fermentation (SSF) steps to obtain bioethanol. Sequential steam explosion (SE), organosolv (OS) pretreatment and soda-anthraquinone pulping (Na/AQ) were performed resulting in an unbleached pulp (UBP), which was successfully converted via SSF to bioethanol with concentrations up to 79 g l−1, which corresponds to a conversion yield of 97% and productivity of 1.09 g l−1 h−1 at 15% total solids. Accordingly, delignification steps by oxygen bleaching are not necessary for bioethanol production. Different industrial softwood (SW) pulps were also tested as reference materials. Total lignin contents up to 4.5% in SW-based pulps had no adverse effects on SSF efficiency.

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Holzforschung is an international scholarly journal that publishes cutting-edge research on the biology, chemistry, physics and technology of wood and wood components. High quality papers about biotechnology and tree genetics are also welcome. Rated year after year as one of the top scientific journals in the category of Pulp and Paper (ISI Journal Citation Index), Holzforschung represents innovative, high quality basic and applied research.

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