Antidiabetic and antihypertensive effect of Virgin Argan Oil in model of neonatal streptozotocin-induced diabetic and l-nitroarginine methylester (l-NAME) hypertensive rats

Said Bellahcen 1 , Zineb Hakkou 1 , Abderrahim Ziyyat 1 , Abdelkhaleq Legssyer 1 , Hassane Mekhfi 1 , Mohammed Aziz 1 ,  and Mohamed Bnouham 1
  • 1 Laboratory of Physiology and Ethnopharmacology, URAC 40, Faculty of Sciences, Department of Biology, University Mohamed Ist, Boulevard Mohamed VI, BP:717, 60 000 Oujda, Morocco
Said Bellahcen, Zineb Hakkou, Abderrahim Ziyyat, Abdelkhaleq Legssyer, Hassane Mekhfi, Mohammed Aziz and Mohamed Bnouham

Abstract

Background: The goal of this study was to examine the effect of Virgin Argan Oil (VAO) obtained from the fruit of Argania spinosa in a model of type 2 diabetes and hypertensive rats. Neonatal diabetes was induced by a single i.p. injection of streptozotocin (90 mg/kg) 2 days after birth. To induce NO-deficient hypertension, the adult diabetic animals were treated with l-nitroarginine methylester (l-NAME) (30 mg/kg/day) given orally for 21 days.

Methods: Following treatment with VAO (21 days), the hyperglycemia decreased to 1.3 ± 0.07 g/l compared with 1.92 ± 0.09 g/l (p < 0.01) in the untreated diabetic-hypertensive rats. The simultaneous administration of VAO with l-NAME prevented the increase in blood pressure during the 3 weeks of treatment. Blood pressure remained constant at 131 ± 1 mm Hg after 21 days – vs 157 ± 0.64 mm Hg in untreated animals (p < 0.001).

Results: The treatment with VAO to diabetic-hypertensive rats caused a significant increase of hepatic glycogen levels (13.3 ± 1.8 vs 6.34 ± 0.75 mg/g tissue in untreated diabetic-hypertensive control group; p < 0.01).

Conclusions: In conclusion, the overall findings indicate that VAO possesses antidiabetic and antihypertensive activity in n-stz/l-NAME rats. This effect may be related to its high content of tocopherols, phenolic compounds, and unsaturated fatty acids.

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The Journal of Complementary and Integrative Medicine focuses on evidence concerning the efficacy and safety of complementary and alternative medical (CAM) whole systems, practices, interventions and natural health products, including herbal medicines.

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