Antiplasmodial potential of homeopathic drugs Chelidonium and nosode against Plasmodium berghei infection

Upma Bagai 1  and Neha S. Walter 1
  • 1 Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh 160014, India
Upma Bagai and Neha S. Walter

Abstract

Background: Malaria remains a major global health concern in developing regions of the world. Homeopathy, a holistic system of medicine, has a lot to offer in protecting against malaria.

Methods: In the present study, antimalarial efficacy of combination of two homeopathic drugs Chelidonium 30 and nosode 30 has been evaluated in vivo against Plasmodium berghei (P. berghei) infection using Peters 4-day test. Biochemical assays have been performed to assess the levels of hepatic and renal function biomarkers upon drug treatment using diagnostic kits.

Results: The combination of the drugs demonstrated considerable in vivo antimalarial activity with chemosuppression of 91.45% on day 7. The combination also significantly (p<0.0005) enhanced the mean survival time of mice which was calculated to be 22.5±6.31 days, whereas it was 8.55±0.83 days in infected control. The increase in levels of the liver function marker enzymes tested in serum of treated mice were significantly less (p<0.0005) than those observed in infected control on day 10. The serum urea and creatinine used for assessment of renal sufficiency were slightly elevated above normal, but were statistically significant (p<0.0005) as compared to infected control.

Conclusions: The study establishes the effectiveness of the combination against P. berghei in vivo along with the safety of the drugs to the liver and kidney functions of the host.

  • 1.

    Bourdy M, Willcox L, Ginsburg H, Rasoanaivo P, Graz B, Deharo E. Ethnopharmacology and malaria: new hypothetical leads or old efficient antimalarials? Int J Parasitol 2008;38:3341.

    • Google Scholar
    • Export Citation
  • 2.

    Lira-Salazar G, Marines-Montiel E, Torres-Monzon J, Hernandez-Hernandez F, Salas-Benito JS. Effects of homeopathic medications Eupatorium perfoliatum and Arsenicum album on parasitaemia of Plasmodium berghei infected mice. Homeopathy 2006;95:2238.

    • Google Scholar
    • Export Citation
  • 3.

    Taborska E, Bochorakova H, Dostal J, Paulova H. The greater celandine Chelidonium majus L. – a review of present knowledge. Ceska Slov Farm 1995;44:715.

    • Google Scholar
    • Export Citation
  • 4.

    Partington T, Ruchira DA, Hennel L, Pittendrigh A, Taylor J. Silent and deadly, prophylaxis and treatment of malaria. Homeopath Pract 2006;Spring:1419.

    • Google Scholar
    • Export Citation
  • 5.

    Rajan A, Bagai U. Effect of homeopathic medicine Chelidonium against blood stage infection of rodent malaria parasite. In: Bandyopadhyay PK, editor. Proceedings of the 22nd national congress of parasitology, Oct 30th to Nov 1st, 2010, Advances in parasitology: a novel approach towards a disease free world. India: University of Kalyani, West Bengal, 2011:2331.

    • Export Citation
  • 6.

    Bagai U, Rajan A, Kaur S. Antimalarial potential of Nosode 30 and 200 against Plasmodium berghei infection in Balb/c mice. J Vector Borne Dis 2012;49:727.

    • Google Scholar
    • Export Citation
  • 7.

    Ernst E. Is homeopathy a clinically valuable approach? Trends Pharmacol Sci 2005;26:5478.

  • 8.

    Garba IH, Gregory U. Serum alkaline phosphatase activity as a potential biomarker for the integrity of the hepatic drainage system in acute falciparum infection. Int J Infect Dis 2005;4:1316.

    • Google Scholar
    • Export Citation
  • 9.

    Nayyar B, Rishi P, Shukla G. Plasmodium berghei induced biochemical alterations in pregnant mice. Nepal Med Coll J 2007;9:469.

    • Google Scholar
    • Export Citation
  • 10.

    White NJ, Ho M. The pathophysiology of malaria. Adv Parasitol 1992;31:84167.

  • 11.

    Adekunle AS, Adekunle OC, Egbewale BE. Serum status of selected biochemical parameters in malaria: an animal model. Biomed Res 2007;18:10913.

    • Google Scholar
    • Export Citation
  • 12.

    Ogbadoyi EO, Tsado RD. Renal and hepatic dysfunction in malaria patients in Minna North Central Nigeria. Online J Health Allied Sci 2009;8:8.

    • Google Scholar
    • Export Citation
  • 13.

    Achliya GS, Wadodkar SG, Dorle AK. Evaluation of hepatoprotective effect of Amalkadi Ghrita against carbon tetrachloride-induced hepatic damage in rats. J Ethnopharmacol 2004;90:22932.

    • Google Scholar
    • Export Citation
  • 14.

    Ewaraiah MC, Satyanarayana I. Hepatoprotective activity of extracts from stem of Mussaenda erythrophylla Lam. against carbon tetrachloride- induced toxicity in rats. JPRHC 2010;2:2331.

    • Google Scholar
    • Export Citation
  • 15.

    Wroblewski F, La Due JS. Serum glutamic oxaloacetic transaminase activity as an index of liver cell injury from cancer. A prelim report. Cancer 1955;8:115563.

    • Google Scholar
    • Export Citation
  • 16.

    Molander DW, Sheppard E, Payne MA. Serum transaminase in liver disease. J Med Assoc 1957;163:14615.

  • 17.

    Zelman S, Wang CC, Appelhanz I. Transaminases in needle aspiration biopsies. Am J Med Sci 1959;237:3233.

  • 18.

    Smith GL, Shlipak MG, Havranek EP, Foody JM, Masoudi FA, Rathore SS, et al. Serum urea nitrogen, creatinine, and estimators of renal function. Arch Intern Med 2006;166:113442.

    • Google Scholar
    • Export Citation
  • 19.

    Kochar DK, Saxena V, Singh N, Kochar SK, Kumar SV, Das A. Plasmodium vivax malaria. Emerg Infect Dis 2005;11:1324.

    • Google Scholar
    • Export Citation
  • 20.

    Davis TM, Pongponratan E, Supanaranond W, Pukrittaykamee S, Helliwell T, Holloway P, et al. Skeleton muscle involvement in falciparum malaria: biochemical and ultrastructure study. Clin Infect Dis 1999;29:8315.

    • Google Scholar
    • Export Citation
  • 21.

    Narayanan S, Appleton HD. Creatinine: a review. Clin Chem 1980;26:111926.

  • 22.

    Mouton R, Holder K. Laboratory tests of renal function. Anaesth Intensive Care Med 2006;7:2403.

  • 23.

    Obianime AW, Aprioku JS. Comparative study of artesunate, ACT’s and their combinants on the biochemical parameters of male guinea pigs. Afr J Biotechnol 2009;8:505965.

    • Google Scholar
    • Export Citation
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The Journal of Complementary and Integrative Medicine focuses on evidence concerning the efficacy and safety of complementary and alternative medical (CAM) whole systems, practices, interventions and natural health products, including herbal medicines.

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